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PDIA2 elisa kit :: Human PDIA2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500687
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDIA2, ELISA Kit

★Popular Item★
 Also Known As   

Human PDIA2 (Protein Disulfide Isomerase A2) ELISA Kit

 Product Gene Name   

PDIA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608012
 3D Structure    ModBase 3D Structure for Q13087
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PDIA2. No significant cross-reactivity or interference between Human PDIA2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PDIA2 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313--20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDIA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDIA2 purchase    MBS2500687 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDIA2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313--20ng/mL in biological research samples containing PDIA2, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2500687 kit is based on PDIA2 antibody-PDIA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDIA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDIA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PDIA2 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDIA2 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDIA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PDIA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDIA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDIA2. You can calculate the concentration of Human PDIA2 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PDIA2 elisa kit    PDIA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500687. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PDIA2. It may not necessarily be applicable to this product.
 NCBI GI #    94966757
 NCBI GeneID    64714
 NCBI Accession #    NP_006840.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006849.2 [Other Products]
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 UniProt Primary Accession #    Q13087 [Other Products]
 UniProt Secondary Accession #    Q2WGM4; Q4TT67; Q6B010; Q96KJ6; Q9BW95; A6ZJ64; B4DI27 [Other Products]
 UniProt Related Accession #    Q13087 [Other Products]
 Molecular Weight    57,850 Da
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 NCBI Official Full Name    protein disulfide-isomerase A2
 NCBI Official Synonym Full Names    protein disulfide isomerase family A, member 2
 NCBI Official Symbol    PDIA2 [Similar Products]
 NCBI Official Synonym Symbols   
PDI; PDA2; PDIP; PDIR
[Similar Products]
 NCBI Protein Information    protein disulfide-isomerase A2; Rho GDP dissociation inhibitor gamma; pancreas-specific protein disulfide isomerase; pancreatic protein disulfide isomerase; protein disulfide isomerase A2; protein disulfide isomerase, pancreatic; protein disulfide isomerase-associated 2
 UniProt Protein Name    Protein disulfide-isomerase A2
 UniProt Synonym Protein Names   
Pancreas-specific protein disulfide isomerase; PDIp
 Protein Family    Protein disulfide-isomerase
 UniProt Gene Name    PDIA2 [Similar Products]
 UniProt Synonym Gene Names    PDIP; PDIp [Similar Products]
 UniProt Entry Name    PDIA2_HUMAN
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 NCBI Summary for PDIA2    Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for PDIA2    PDIA2: Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. Belongs to the protein disulfide isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; EC 5.3.4.1; Isomerase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: disulfide oxidoreductase activity; protein binding; protein disulfide isomerase activity; peptidyl-proline 4-dioxygenase activity; steroid binding

Biological Process: protein folding; cell redox homeostasis; peptidyl-proline hydroxylation; protein retention in ER
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 Research Articles on PDIA2    1. The redox-regulated open/closed conformational switch of hPDI endows the protein with versatile target-binding capacities for its enzymatic and chaperone functions.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDIA2 elisa kitPathways associated with PDIA2 elisa kit
 Reference Product  PubMed Publications
 LRP1 elisa kit  >1 publications with PDIA2 and LRP1
 Products by Pathway  Pathway Diagram
 Statin Pathway antibodies  Statin Pathway Diagram
Diseases associated with PDIA2 elisa kitOrgans/Tissues associated with PDIA2 elisa kit
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >1 publications with PDIA2 and Congenital Abnormalities
 Death Antibodies  >1 publications with PDIA2 and Death
 Hypertension Antibodies  >1 publications with PDIA2 and Hypertension
 Heart Diseases Antibodies  >1 publications with PDIA2 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >3 publications with PDIA2 and Pancreas
 Brain Antibodies  >2 publications with PDIA2 and Brain
 Stomach Antibodies  >1 publications with PDIA2 and Stomach
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