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PDLIM5 elisa kit :: Human PDZ and LIM domain 5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS900863
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

PDZ and LIM domain 5 (PDLIM5), ELISA Kit

★Popular Item★
 Also Known As   

Human PDZ and LIM domain protein 5, PDLIM5 ELISA Kit

 Product Synonym Names    Human PDZ and LIM domain protein 5 (PDLIM5) ELISA kit; ENH; ENH1; L9; LIM; enigma homolog; enigma-like LIM domain protein; PDZ and LIM domain 5
 Product Gene Name   

PDLIM5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS900863 Testing Data    Testing Data PDF
 OMIM    605904
 3D Structure    ModBase 3D Structure for Q96HC4
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PDLIM5. No significant cross-reactivity or interference between human PDLIM5 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Sandwich
 Detection Range    18.75 pg/ml -1200 pg/ml.
 Sensitivity    The minimum detectable dose of human PDLIM5 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PDLIM5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDLIM5 purchase    MBS900863 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PDZ and LIM domain 5 (PDLIM5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.75 pg/ml -1200 pg/ml in biological research samples containing PDLIM5, with an estimated sensitivity of The minimum detectable dose of human PDLIM5 is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS900863 kit is based on PDLIM5 antibody-PDLIM5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDLIM5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDLIM5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PDLIM5 elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDLIM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDLIM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDLIM5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDLIM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PDLIM5 elisa kit    PDLIM5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS900863. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PDLIM5. It may not necessarily be applicable to this product.
 NCBI GI #    58533159
 NCBI GeneID    10611
 NCBI Accession #    NP_001011515.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001011515.2 [Other Products]
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 UniProt Primary Accession #    Q96HC4 [Other Products]
 UniProt Secondary Accession #    O60705; Q56VN4; Q5UW38; Q8WVK0; A8K6F9; D6RB78; E9PBF5 [Other Products]
 UniProt Related Accession #    Q96HC4 [Other Products]
 Molecular Weight    63,945 Da
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 NCBI Official Full Name    PDZ and LIM domain protein 5 isoform d
 NCBI Official Synonym Full Names    PDZ and LIM domain 5
 NCBI Official Symbol    PDLIM5 [Similar Products]
 NCBI Official Synonym Symbols   
L9; ENH; LIM; ENH1
[Similar Products]
 NCBI Protein Information    PDZ and LIM domain protein 5; enigma homolog; enigma-like LIM domain protein; enigma-like PDZ and LIM domains protein
 UniProt Protein Name    PDZ and LIM domain protein 5
 UniProt Synonym Protein Names   
Enigma homolog; Enigma-like PDZ and LIM domains protein
 Protein Family    PDZ and LIM domain protein
 UniProt Gene Name    PDLIM5 [Similar Products]
 UniProt Synonym Gene Names    ENH; L9 [Similar Products]
 UniProt Entry Name    PDLI5_HUMAN
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 NCBI Summary for PDLIM5    This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
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 UniProt Comments for PDLIM5    LIM: an adaptor protein of the Enigma family. Contains three LIM domains and one PDZ domain. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. Enigma family proteins possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: postsynaptic membrane; neuron projection; membrane; postsynaptic density; cytosol; Z disc; cell junction; actin cytoskeleton

Molecular Function: actinin binding; protein kinase C binding; zinc ion binding; protein N-terminus binding; actin binding

Biological Process: regulation of synaptogenesis
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 Research Articles on PDLIM5    1. It can be deduced that the nonsynonymous rs7690296 polymorphism of PDLIM5 could play an important role in the pathophysiology of both bipolar disorder and schizophrenia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDLIM5 elisa kitDiseases associated with PDLIM5 elisa kit
 Reference Product  PubMed Publications
 PRKCG elisa kit  >1 publications with PDLIM5 and PRKCG
 CACNA1B elisa kit  >1 publications with PDLIM5 and CACNA1B
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3 publications with PDLIM5 and Cardiovascular Diseases
 Heart Diseases Antibodies  >2 publications with PDLIM5 and Heart Diseases
 Lung Neoplasms Antibodies  >1 publications with PDLIM5 and Lung Neoplasms
 Breast Neoplasms Antibodies  >1 publications with PDLIM5 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with PDLIM5 and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >1 publications with PDLIM5 and Nervous System Diseases
 Hypertrophy Antibodies  >1 publications with PDLIM5 and Hypertrophy
 Hyperplasia Antibodies  >1 publications with PDLIM5 and Hyperplasia
 Fibrosis Antibodies  >1 publications with PDLIM5 and Fibrosis
 Carcinoma, Squamous Cell Antibodies  >1 publications with PDLIM5 and Carcinoma, Squamous Cell
Organs/Tissues associated with PDLIM5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with PDLIM5 and Brain
 Blood Antibodies  >4 publications with PDLIM5 and Blood
 Nerve Antibodies  >3 publications with PDLIM5 and Nerve
 Muscle Antibodies  >3 publications with PDLIM5 and Muscle
 Prostate Antibodies  >2 publications with PDLIM5 and Prostate
 Heart Antibodies  >2 publications with PDLIM5 and Heart
 Thyroid Antibodies  >1 publications with PDLIM5 and Thyroid
 Mouth Antibodies  >1 publications with PDLIM5 and Mouth
 Lung Antibodies  >1 publications with PDLIM5 and Lung
 Uncharacterized Tissue Antibodies  >1 publications with PDLIM5 and Uncharacterized Tissue
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