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| Product Name
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PI-PLC X domain-containing protein 2 (PLCXD2), ELISA Kit
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| Also Known As
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Human PI-PLC X domain-containing protein 2 (PLCXD2) ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Request for Current Manual Insert
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| OMIM
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AK056141 mRNA
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| 3D Structure
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ModBase 3D Structure for Q0VAA5
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| Species Reactivity
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Human
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of Human PLCXD2. No significant cross-reactivity or interference between Human PLCXD2 and analogues was observed.
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| Samples
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Serum, Plasma, Other biological fluids
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| Assay Type
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Sandwich
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| Sample Volume
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1-200 uL
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| Precision
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Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
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| Detection Wavelength
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450 nm
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| Preparation and Storage
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Store at 2-8 degree C.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of PLCXD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for PLCXD2 purchase
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MBS280838 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PI-PLC X domain-containing protein 2 (PLCXD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCXD2. The ELISA analytical biochemical technique of the MBS280838 kit is based on PLCXD2 antibody-PLCXD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCXD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCXD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLCXD2 in samples. An antibody specific for PLCXD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLCXD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLCXD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLCXD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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| NCBI/Uniprot data below describe general gene information for PLCXD2. It may not necessarily be applicable to this product. |
| NCBI GI #
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297515471
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| NCBI GeneID
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257068
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| NCBI Accession #
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NP_001172035.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001185106.1
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[Other Products]
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| UniProt Primary Accession #
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Q0VAA5
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[Other Products]
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| UniProt Secondary Accession #
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Q96N12
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[Other Products]
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| UniProt Related Accession #
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Q0VAA5
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[Other Products]
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| Molecular Weight
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34,767 Da
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| NCBI Official Full Name
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PI-PLC X domain-containing protein 2 isoform a
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| NCBI Official Synonym Full Names
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phosphatidylinositol specific phospholipase C X domain containing 2
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| NCBI Official Symbol
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PLCXD2
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[Similar Products]
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| NCBI Protein Information
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PI-PLC X domain-containing protein 2
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| UniProt Protein Name
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PI-PLC X domain-containing protein 2
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| Protein Family
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PI-PLC X domain-containing protein
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| UniProt Gene Name
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PLCXD2
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[Similar Products]
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| UniProt Comments for PLCXD2
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PLCXD2: 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 3q13.2
Cellular Component: nucleus
Molecular Function: phosphoric diester hydrolase activity; signal transducer activity
Biological Process: lipid catabolic process; signal transduction
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| Research Articles on PLCXD2
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1. Observational study of gene-disease association. (HuGE Navigator)
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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ELISA Kit 
