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PKC elisa kit :: Human PKC ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2514873 PKC elisa kit
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PKC, ELISA Kit

 Also Known As   

Human PKC (Protein Kinase C) ELISA Kit

 Product Gene Name   

PKC elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    128200
 3D Structure    ModBase 3D Structure for Q7Z6L0
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinantHumanPKC. No significant cross-reactivity or interference between HumanPKC and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich (Quantitative)
 Detection Range    0.313-20ng/mL
 Sensitivity    0.188ng/mL
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 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PKC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PKC purchase    MBS2514873 is a ready-to-use microwell, strip plate Sandwich (Quantitative) ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PKC, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing PKC, with an estimated sensitivity of 0.188ng/mL. The ELISA analytical biochemical technique of the MBS2514873 kit is based on PKC antibody-PKC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PKC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PKC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PKC elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanPKCconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PKC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PKCand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PKC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PKC.You can calculate the concentration of PKCin the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2514873
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NCBI/Uniprot data below describe general gene information for PKC. It may not necessarily be applicable to this product.
 NCBI GI #    156523246
 NCBI GeneID    112476
 NCBI Accession #    NP_660282.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_145239.2 [Other Products]
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 UniProt Primary Accession #    Q7Z6L0 [Other Products]
 UniProt Secondary Accession #    Q8N2N8; Q8NAQ7; Q8ND36; Q96FA8; A8K8M8 [Other Products]
 UniProt Related Accession #    Q7Z6L0 [Other Products]
 Molecular Weight    30,653 Da
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 NCBI Official Full Name    proline-rich transmembrane protein 2 isoform 1
 NCBI Official Synonym Full Names    proline-rich transmembrane protein 2
 NCBI Official Symbol    PRRT2 [Similar Products]
 NCBI Official Synonym Symbols   
PKC; EKD1; ICCA; BFIC2; BFIS2; DSPB3; DYT10; FICCA; IFITMD1
[Similar Products]
 NCBI Protein Information    proline-rich transmembrane protein 2; dispanin subfamily B member 3; infantile convulsions and paroxysmal choreoathetosis; interferon induced transmembrane protein domain containing 1
 UniProt Protein Name    Proline-rich transmembrane protein 2
 UniProt Synonym Protein Names   
Dispanin subfamily B member 3; DSPB3
 UniProt Gene Name    PRRT2 [Similar Products]
 UniProt Synonym Gene Names    DSPB3 [Similar Products]
 UniProt Entry Name    PRRT2_HUMAN
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 NCBI Summary for PKC    This gene encodes a transmembrane protein containing a proline-rich domain in its N-terminal half. Studies in mice suggest that it is predominantly expressed in brain and spinal cord in embryonic and postnatal stages. Mutations in this gene are associated with episodic kinesigenic dyskinesia-1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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 UniProt Comments for PKC    PRRT2: Defects in PRRT2 are the cause of episodic kinesigenic dyskinesia type 1 (EKD1). An autosomal dominant neurologic condition characterized by recurrent and brief attacks of abnormal involuntary movements, triggered by sudden voluntary movement. These attacks usually have onset during childhood or early adulthood and can involve dystonic postures, chorea, or athetosis. Disease-causing mutations that produce truncation of the C-terminus of the protein alter subcellular location, from plasma membrane to cytosplasm (PubMed:22101681). Defects in PRRT2 are the cause of convulsions, familial infantile, with paroxysmal choreoathetosis (ICCA). A syndrome characterized by clinical features of benign familial infantile seizures and episodic kinesigenic dyskinesia. Benign familial infantile seizures is a disorder characterized by afebrile seizures occurring during the first year of life, without neurologic sequelae. Paroxysmal choreoathetosis is a disorder of involuntary movements characterized by attacks that occur spontaneously or are induced by a variety of stimuli. Defects in PRRT2 are the cause of seizures, benign familial infantile type 2 (BFIS2). An autosomal dominant disorder in which afebrile seizures occur in clusters during the first year of life, without neurologic sequelae. Belongs to the CD225/Dispanin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: plasma membrane; integral to membrane; synapse; cell junction

Biological Process: neuromuscular process controlling posture; response to biotic stimulus

Disease: Episodic Kinesigenic Dyskinesia 1
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 Research Articles on PKC    1. Two novel mutations in PRRT2 were revealed in paroxysmal dyskinesia and choreoathetosis syndrome.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PKC elisa kitDiseases associated with PKC elisa kit
 Reference Product  PubMed Publications
 PNKD elisa kit  >6 publications with PKC and PNKD
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >53 publications with PKC and Brain Diseases
 Familial paroxysmal dystonia Antibodies  >14 publications with PKC and Familial paroxysmal dystonia
 Intellectual Disability Antibodies  >7 publications with PKC and Intellectual Disability
 Atrophy Antibodies  >2 publications with PKC and Atrophy
 Learning Disorders Antibodies  >2 publications with PKC and Learning Disorders
 Disease Models, Animal Antibodies  >1 publications with PKC and Disease Models, Animal
 Cognition Disorders Antibodies  >1 publications with PKC and Cognition Disorders
 Motor Skills Disorders Antibodies  >1 publications with PKC and Motor Skills Disorders
Organs/Tissues associated with PKC elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >22 publications with PKC and Brain
 Muscle Antibodies  >5 publications with PKC and Muscle
 Kidney Antibodies  >1 publications with PKC and Kidney
 Embryonic Tissue Antibodies  >1 publications with PKC and Embryonic Tissue
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