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POT1 elisa kit :: Human POT1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2515741
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

POT1, ELISA Kit

★Popular Item★
 Also Known As   

Human POT1 (Protection of Telomeres Protein 1) ELISA Kit

 Product Gene Name   

POT1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    606478
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinantHumanPOT1. No significant cross-reactivity or interference between HumanPOT1 and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Min: 0.188ng/mL; Max: 20ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of POT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for POT1 purchase    MBS2515741 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the POT1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing POT1, with an estimated sensitivity of Min: 0.188ng/mL; Max: 20ng/mL. The ELISA analytical biochemical technique of the MBS2515741 kit is based on POT1 antibody-POT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for POT1 elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanPOT1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to POT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for POT1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain POT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of POT1.You can calculate the concentration of POT1in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of POT1 elisa kit    POT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2515741. Click to request current manual
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NCBI/Uniprot data below describe general gene information for POT1. It may not necessarily be applicable to this product.
 NCBI GI #    98991776
 NCBI GeneID    25913
 NCBI Accession #    NP_056265.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_015450.2 [Other Products]
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 UniProt Secondary Accession #    O95018; Q5MJ36; Q9H662; Q9NW19; Q9UG95 [Other Products]
 UniProt Related Accession #    Q9NUX5 [Other Products]
 Molecular Weight    60,534 Da
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 NCBI Official Full Name    protection of telomeres protein 1 isoform 1
 NCBI Official Synonym Full Names    protection of telomeres 1
 NCBI Official Symbol    POT1 [Similar Products]
 NCBI Official Synonym Symbols   
CMM10; HPOT1
[Similar Products]
 NCBI Protein Information    protection of telomeres protein 1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog
 UniProt Protein Name    Protection of telomeres protein 1
 UniProt Synonym Protein Names   
POT1-like telomere end-binding protein
 Protein Family    Potassium transporter
 UniProt Gene Name    POT1 [Similar Products]
 UniProt Synonym Gene Names    hPot1 [Similar Products]
 UniProt Entry Name    POTE1_HUMAN
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 NCBI Summary for POT1    This gene is a member of the telombin family and encodes a nuclear protein involved in telomere maintenance. Specifically, this protein functions as a member of a multi-protein complex that binds to the TTAGGG repeats of telomeres, regulating telomere length and protecting chromosome ends from illegitimate recombination, catastrophic chromosome instability, and abnormal chromosome segregation. Increased transcriptional expression of this gene is associated with stomach carcinogenesis and its progression. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for POT1    POT1: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA- binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'- TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity by itself. In contrast, the ACD-POT1 heterodimer enhances telomere elongation by increasing telomerase processivity. Belongs to the telombin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 7q31.33

Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; cytoplasm; nucleus

Molecular Function: protein binding; single-stranded telomeric DNA binding; DEAD/H-box RNA helicase binding

Biological Process: positive regulation of helicase activity; negative regulation of telomerase activity; telomere assembly; telomere capping; telomere maintenance via telomerase; positive regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; telomere maintenance; DNA duplex unwinding; positive regulation of telomere maintenance via telomerase

Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 10
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 Research Articles on POT1    1. Expression of shelterin component POT1 is associated with decreased telomere length and immunity condition in humans with severe aplastic anemia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with POT1 elisa kitPathways associated with POT1 elisa kit
 Reference Product  PubMed Publications
 TPP1 elisa kit  >78 publications with POT1 and TPP1
 TERF2 elisa kit  >30 publications with POT1 and TERF2
 TINF2 elisa kit  >26 publications with POT1 and TINF2
 TERF1 elisa kit  >12 publications with POT1 and TERF1
 TERF2IP elisa kit  >12 publications with POT1 and TERF2IP
 ACD elisa kit  >8 publications with POT1 and ACD
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Damage/Telomere Stress Induced Senescence Pathway antibodies  DNA Damage/Telomere Stress Induced Senescence Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Synapsis Pathway antibodies  Meiotic Synapsis Pathway Diagram
 Packaging Of Telomere Ends Pathway antibodies  Packaging Of Telomere Ends Pathway Diagram
 Regulation Of Telomerase Pathway antibodies  Regulation Of Telomerase Pathway Diagram
 Shelterin Complex Pathway antibodies  Shelterin Complex Pathway Diagram
Diseases associated with POT1 elisa kitOrgans/Tissues associated with POT1 elisa kit
 Disease Name  Pubmed Publications
 Chromosome Aberrations Antibodies  >22 publications with POT1 and Chromosome Aberrations
 Carcinoma Antibodies  >17 publications with POT1 and Carcinoma
 Leukemia, Lymphoid Antibodies  >7 publications with POT1 and Leukemia, Lymphoid
 Adenocarcinoma Antibodies  >6 publications with POT1 and Adenocarcinoma
 Melanoma Antibodies  >6 publications with POT1 and Melanoma
 Intestinal Neoplasms Antibodies  >5 publications with POT1 and Intestinal Neoplasms
 Liver Neoplasms Antibodies  >5 publications with POT1 and Liver Neoplasms
 Liver Diseases Antibodies  >4 publications with POT1 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with POT1 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >4 publications with POT1 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with POT1 and Blood
 Bone Antibodies  >12 publications with POT1 and Bone
 Bone Marrow Antibodies  >8 publications with POT1 and Bone Marrow
 Liver Antibodies  >6 publications with POT1 and Liver
 Lung Antibodies  >5 publications with POT1 and Lung
 Lymph Node Antibodies  >5 publications with POT1 and Lymph Node
 Stomach Antibodies  >5 publications with POT1 and Stomach
 Brain Antibodies  >5 publications with POT1 and Brain
 Kidney Antibodies  >4 publications with POT1 and Kidney
 Thyroid Antibodies  >4 publications with POT1 and Thyroid
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