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PRKG1 elisa kit :: Human PRKG1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2504113
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PRKG1, ELISA Kit

★Popular Item★
 Also Known As   

Human PRKG1 (Protein Kinase, cGMP Dependent Type I) ELISA Kit

 Product Gene Name   

PRKG1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    176894
 3D Structure    ModBase 3D Structure for Q13976
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PRKG1. No significant cross-reactivity or interference between Human PRKG1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PRKG1 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156--10ng/mL
 Sensitivity    0.094ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRKG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRKG1 purchase    MBS2504113 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PRKG1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156--10ng/mL in biological research samples containing PRKG1, with an estimated sensitivity of 0.094ng/mL. The ELISA analytical biochemical technique of the MBS2504113 kit is based on PRKG1 antibody-PRKG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRKG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRKG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PRKG1 elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRKG1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRKG1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PRKG1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRKG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PRKG1. You can calculate the concentration of Human PRKG1 in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PRKG1 elisa kit    PRKG1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2504113. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PRKG1. It may not necessarily be applicable to this product.
 NCBI GI #    38571600
 NCBI GeneID    5592
 NCBI Accession #    AAH62688.1 [Other Products]
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 UniProt Primary Accession #    Q13976 [Other Products]
 UniProt Secondary Accession #    P14619; Q5JP05; Q5JSJ6; Q6P5T7; A5YM56; B3KSF3; E2PU10 [Other Products]
 UniProt Related Accession #    Q13976 [Other Products]
 Molecular Weight    44,185 Da
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 NCBI Official Full Name    PRKG1 protein
 NCBI Official Synonym Full Names    protein kinase, cGMP-dependent, type I
 NCBI Official Symbol    PRKG1 [Similar Products]
 NCBI Official Synonym Symbols   
1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
[Similar Products]
 NCBI Protein Information    cGMP-dependent protein kinase 1; protein kinase, cGMP-dependent, regulatory, type I, beta
 UniProt Protein Name    cGMP-dependent protein kinase 1
 UniProt Synonym Protein Names   
cGMP-dependent protein kinase I; cGKI
 Protein Family    cGMP-dependent protein kinase
 UniProt Gene Name    PRKG1 [Similar Products]
 UniProt Synonym Gene Names    PRKG1B; PRKGR1A; PRKGR1B; cGK 1; cGK1; cGKI [Similar Products]
 UniProt Entry Name    KGP1_HUMAN
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 NCBI Summary for PRKG1    Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]
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 UniProt Comments for PRKG1    PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.12; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKG family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: Golgi apparatus; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; cGMP-dependent protein kinase activity; calcium channel regulator activity; cGMP binding; ATP binding

Biological Process: regulation of GTPase activity; relaxation of vascular smooth muscle; cGMP-mediated signaling; forebrain development; dendrite development; neuron migration; signal transduction; actin cytoskeleton organization and biogenesis; blood coagulation; protein amino acid phosphorylation
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 Research Articles on PRKG1    1. These findings characterize PKG as a novel regulator of AR-mediated transcription by enhancing AR cofactor p44/WDR77's function.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PRKG1 elisa kitPathways associated with PRKG1 elisa kit
 Reference Product  PubMed Publications
 VASP elisa kit  >6 publications with PRKG1 and VASP
 PDE5A elisa kit  >5 publications with PRKG1 and PDE5A
 MRVI1 elisa kit  >4 publications with PRKG1 and MRVI1
 KCNMA1 elisa kit  >1 publications with PRKG1 and KCNMA1
 PPP1R12A elisa kit  >1 publications with PRKG1 and PPP1R12A
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Ca2+ Pathway antibodies  Ca2+ Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Long-term Depression Pathway antibodies  Long-term Depression Pathway Diagram
 Long-term Depression Pathway antibodies  Long-term Depression Pathway Diagram
Diseases associated with PRKG1 elisa kitOrgans/Tissues associated with PRKG1 elisa kit
 Disease Name  Pubmed Publications
 Hypertension Antibodies  >23 publications with PRKG1 and Hypertension
 Heart Diseases Antibodies  >11 publications with PRKG1 and Heart Diseases
 Hypertrophy Antibodies  >9 publications with PRKG1 and Hypertrophy
 Inflammation Antibodies  >6 publications with PRKG1 and Inflammation
 Cardiomegaly Antibodies  >6 publications with PRKG1 and Cardiomegaly
 Pain Antibodies  >6 publications with PRKG1 and Pain
 Hypertension, Pulmonary Antibodies  >6 publications with PRKG1 and Hypertension, Pulmonary
 Brain Diseases Antibodies  >5 publications with PRKG1 and Brain Diseases
 Hyperplasia Antibodies  >4 publications with PRKG1 and Hyperplasia
 Necrosis Antibodies  >4 publications with PRKG1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >68 publications with PRKG1 and Muscle
 Vascular Antibodies  >59 publications with PRKG1 and Vascular
 Heart Antibodies  >23 publications with PRKG1 and Heart
 Brain Antibodies  >23 publications with PRKG1 and Brain
 Lung Antibodies  >12 publications with PRKG1 and Lung
 Kidney Antibodies  >10 publications with PRKG1 and Kidney
 Testis Antibodies  >8 publications with PRKG1 and Testis
 Intestine Antibodies  >5 publications with PRKG1 and Intestine
 Ganglia Antibodies  >5 publications with PRKG1 and Ganglia
 Prostate Antibodies  >4 publications with PRKG1 and Prostate
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