Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PSA/KLK3 rightarrow Human PSA/KLK3  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Histone H2AZ (H2AFZ) Antibody
 • Dna Damage Inducible Transcript 3 (DDIT3) ELISA Kit
 • Cholecystokinin B Receptor (CCKBR) ELISA Kit
 • sCD4 ELISA Kit
 • Actinin Alpha 2 (ACTN2) ELISA Kit
 • T-Box Protein 3 (TBX3) ELISA Kit
 • Estrogen Receptor (ER) ELISA Kit
 • 17-Alpha Hydroxylase (S17AH) ELISA Kit
 • Ceruletide-Cerulein-Caerule in (CL) ELISA Kit
 • Macrophage Inflammatory Protein-1gamma (MIP-1gamma) ELISA Kit
 • Inhibin Beta B (INHbetaB) ELISA Kit
 • Cyclin A (CA) ELISA Kit
 • Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit
 • Interleukin 33 (IL-33) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PSA/KLK3 elisa kit :: Human PSA/KLK3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500373 PSA/KLK3 elisa kit
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PSA/KLK3, ELISA Kit

 Also Known As   

Human PSA/KLK3 (Prostate Specific Antigen) ELISA Kit

 Product Gene Name   

PSA/KLK3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    176820
 3D Structure    ModBase 3D Structure for P07288
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This kit recognizes natural and recombinant Human PSA/KLK3. No significant cross-reactivity or interference between Human PSA/KLK3 and analogues was observed.
Section Bar
 Samples    Serum, plasma and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.938-60ng/mL
 Sensitivity    The minimum detectable dose of Human PSA/KLK3 is 0.563ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
 Intended Uses    This ELISA kit applies to the in vitro quantitative determination of Human PSA/KLK3 concentrations in serum, plasma and other biological fluids.
Section Bar
 Preparation and Storage    Store at 4 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PSA/KLK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PSA/KLK3 purchase    MBS2500373 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PSA/KLK3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.938-60ng/mL in biological research samples containing PSA/KLK3, with an estimated sensitivity of The minimum detectable dose of Human PSA/KLK3 is 0.563ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero). The ELISA analytical biochemical technique of the MBS2500373 kit is based on PSA/KLK3 antibody-PSA/KLK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSA/KLK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSA/KLK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for PSA/KLK3 elisa kit

   Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PSA/KLK3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PSA/KLK3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PSA/KLK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PSA/KLK3. You can calculate the concentration of PSA/KLK3 in the samples by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2500373. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PSA/KLK3. It may not necessarily be applicable to this product.
 NCBI GI #    4502173
 NCBI GeneID    354
 NCBI Accession #    NP_001639.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001648.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P07288 [Other Products]
 UniProt Secondary Accession #    Q15096; Q16272; Q86TG8; Q8IXI4; C9JXH3; G3V0H4; G3XAE3 [Other Products]
 UniProt Related Accession #    P07288 [Other Products]
 Molecular Weight    25,116 Da
Table BarTOPTable Bar
 NCBI Official Full Name    prostate-specific antigen isoform 1 preproprotein
 NCBI Official Synonym Full Names    kallikrein-related peptidase 3
 NCBI Official Symbol    KLK3 [Similar Products]
 NCBI Official Synonym Symbols   
APS; PSA; hK3; KLK2A1
[Similar Products]
 NCBI Protein Information    prostate-specific antigen; P-30 antigen; gamma-seminoprotein; kallikrein-3; prostate specific antigen; semenogelase; seminin
 UniProt Protein Name    Prostate-specific antigen
 UniProt Synonym Protein Names   
Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase
 UniProt Gene Name    KLK3 [Similar Products]
 UniProt Synonym Gene Names    APS; PSA; Seminin [Similar Products]
 UniProt Entry Name    KLK3_HUMAN
Section Bar
 NCBI Summary for PSA/KLK3    Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for PSA/KLK3    PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.77; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis
Table BarTOPTable Bar
 Research Articles on PSA/KLK3    1. Suggest that the occurrence of PCa is related to the genotype of KLK3 SNPs rs2735839.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PSA/KLK3 elisa kitPathways associated with PSA/KLK3 elisa kit
 Reference Product  PubMed Publications
 TP53 elisa kit  >2 publications with PSA/KLK3 and TP53
 ERBB2 elisa kit  >2 publications with PSA/KLK3 and ERBB2
 IL6 elisa kit  >1 publications with PSA/KLK3 and IL6
 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Prostate Cancer Pathway antibodies  Prostate Cancer Pathway Diagram
 Regulation Of Androgen Receptor Activity Pathway antibodies  Regulation Of Androgen Receptor Activity Pathway Diagram
 Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway antibodies  Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway Diagram
Diseases associated with PSA/KLK3 elisa kitOrgans/Tissues associated with PSA/KLK3 elisa kit
 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >79 publications with PSA/KLK3 and Prostatic Neoplasms
 Adenocarcinoma Antibodies  >15 publications with PSA/KLK3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >14 publications with PSA/KLK3 and Breast Neoplasms
 Disease Progression Antibodies  >10 publications with PSA/KLK3 and Disease Progression
 Hyperplasia Antibodies  >9 publications with PSA/KLK3 and Hyperplasia
 Prostatic Hyperplasia Antibodies  >9 publications with PSA/KLK3 and Prostatic Hyperplasia
 Ovarian Neoplasms Antibodies  >7 publications with PSA/KLK3 and Ovarian Neoplasms
 Kidney Diseases Antibodies  >6 publications with PSA/KLK3 and Kidney Diseases
 Neoplasm Metastasis Antibodies  >6 publications with PSA/KLK3 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >6 publications with PSA/KLK3 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >143 publications with PSA/KLK3 and Prostate
 Brain Antibodies  >5 publications with PSA/KLK3 and Brain
 Bone Antibodies  >5 publications with PSA/KLK3 and Bone
 Salivary Gland Antibodies  >4 publications with PSA/KLK3 and Salivary Gland
 Muscle Antibodies  >2 publications with PSA/KLK3 and Muscle
 Bone Marrow Antibodies  >2 publications with PSA/KLK3 and Bone Marrow
 Nerve Antibodies  >2 publications with PSA/KLK3 and Nerve
 Adrenal Gland Antibodies  >1 publications with PSA/KLK3 and Adrenal Gland
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions