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PUMA elisa kit :: Human PUMA ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500852
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PUMA, ELISA Kit

★Popular Item★
 Also Known As   

Human PUMA (P53 Upregulated Modulator of Apoptosis) ELISA Kit

 Product Gene Name   

PUMA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q80ZG6
 Species Reactivity    Human
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 Specificity    This kit recognizes natural and some recombinant Human PUMA. No significant cross-reactivity or interference between Human PUMA and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PUMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PUMA purchase    MBS2500852 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PUMA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing PUMA, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2500852 kit is based on PUMA antibody-PUMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PUMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PUMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PUMA elisa kit

   Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human PUMA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PUMA. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human PUMA and Avidin- Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human PUMA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Human PUMA. The concentration of Human PUMA in samples can be calculated by comparing the OD of the samples with the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PUMA elisa kit    PUMA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500852. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PUMA. It may not necessarily be applicable to this product.
 NCBI GI #    27542559
 NCBI GeneID    317673
 NCBI Accession #    AAO16862.1 [Other Products]
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 UniProt Primary Accession #    Q80ZG6 [Other Products]
 UniProt Related Accession #    Q80ZG6 [Other Products]
 Molecular Weight    20,718 Da
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 NCBI Official Full Name    PUMA
 NCBI Official Synonym Full Names    Bcl-2 binding component 3
 NCBI Official Symbol    Bbc3 [Similar Products]
 NCBI Official Synonym Symbols   
Puma
[Similar Products]
 NCBI Protein Information    bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
 UniProt Protein Name    Bcl-2-binding component 3
 UniProt Synonym Protein Names   
p53 up-regulated modulator of apoptosis
 UniProt Gene Name    Bbc3 [Similar Products]
 UniProt Synonym Gene Names    Puma [Similar Products]
 UniProt Entry Name    BBC3_RAT
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 UniProt Comments for PUMA    PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: mitochondrion; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; glial cell differentiation; positive regulation of apoptosis; positive regulation of protein homooligomerization; release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; brain development; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus; negative regulation of growth
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 Research Articles on PUMA    1. Puma is activated by both p53 and PI3K/Akt/FoxO3a pathways and cooperates with Bim to induce neuron death in response to Abeta(1-42)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PUMA elisa kitDiseases associated with PUMA elisa kit
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
 Measles Pathway antibodies  Measles Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
 P53 Signaling Pathway antibodies  P53 Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >73 publications with PUMA and Carcinoma
 Adenocarcinoma Antibodies  >33 publications with PUMA and Adenocarcinoma
 Breast Neoplasms Antibodies  >32 publications with PUMA and Breast Neoplasms
 Lung Neoplasms Antibodies  >24 publications with PUMA and Lung Neoplasms
 Necrosis Antibodies  >20 publications with PUMA and Necrosis
 Liver Diseases Antibodies  >18 publications with PUMA and Liver Diseases
 Lung Diseases Antibodies  >17 publications with PUMA and Lung Diseases
 Nervous System Diseases Antibodies  >16 publications with PUMA and Nervous System Diseases
 Carcinoma, Squamous Cell Antibodies  >13 publications with PUMA and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >13 publications with PUMA and Brain Diseases
Organs/Tissues associated with PUMA elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >13 publications with PUMA and Kidney
 Mixed Antibodies  >1 publications with PUMA and Mixed
 Ovary Antibodies  >1 publications with PUMA and Ovary
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