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PYCARD elisa kit :: Human PYD and CARD domain containing ELISA Kit

Scan QR to view Datasheet Catalog #    MBS918297
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PYD and CARD domain containing (PYCARD), ELISA Kit

★Popular Item★
 Also Known As   

Human Apoptosis-associated speck-like protein containing a CARD, PYCARD ELISA Kit

 Product Synonym Names    Human Apoptosis-associated speck-like protein containing a CARD (PYCARD) ELISA kit; ASC; CARD5; MGC10332; TMS; TMS-1; TMS1; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1; PYD and CARD domain containing
 Product Gene Name   

PYCARD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS918297 Testing Data    Testing Data PDF
 OMIM    606838
 3D Structure    ModBase 3D Structure for Q9ULZ3
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human PYCARD. No significant cross-reactivity or interference between human PYCARD and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates
 Assay Type    Quantitative Sandwich
 Detection Range    15.6 pg/ml -1000 pg/ml
 Sensitivity    Typically less than 3.9 pg/ml.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PYCARD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PYCARD purchase    MBS918297 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PYD and CARD domain containing (PYCARD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml -1000 pg/ml in biological research samples containing PYCARD, with an estimated sensitivity of Typically less than 3.9 pg/ml. The ELISA analytical biochemical technique of the MBS918297 kit is based on PYCARD antibody-PYCARD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PYCARD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PYCARD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PYCARD elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PYCARD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYCARD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCARD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCARD bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of PYCARD elisa kit    PYCARD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS918297. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PYCARD. It may not necessarily be applicable to this product.
 NCBI GI #    10835256
 NCBI GeneID    29108
 NCBI Accession #    NP_037390.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013258.4 [Other Products]
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 UniProt Primary Accession #    Q9ULZ3 [Other Products]
 UniProt Secondary Accession #    Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8 [Other Products]
 UniProt Related Accession #    Q9ULZ3 [Other Products]
 Molecular Weight    15,030 Da
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 NCBI Official Full Name    apoptosis-associated speck-like protein containing a CARD isoform a
 NCBI Official Synonym Full Names    PYD and CARD domain containing
 NCBI Official Symbol    PYCARD [Similar Products]
 NCBI Official Synonym Symbols   
ASC; TMS; TMS1; CARD5; TMS-1
[Similar Products]
 NCBI Protein Information    apoptosis-associated speck-like protein containing a CARD; target of methylation-induced silencing 1; caspase recruitment domain-containing protein 5
 UniProt Protein Name    Apoptosis-associated speck-like protein containing a CARD
 UniProt Synonym Protein Names   
Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
 Protein Family    Apoptosis-associated speck-like protein
 UniProt Gene Name    PYCARD [Similar Products]
 UniProt Synonym Gene Names    ASC; CARD5; TMS1; hASC [Similar Products]
 UniProt Entry Name    ASC_HUMAN
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 NCBI Summary for PYCARD    This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PYCARD    PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: mitochondrion; cell soma; endoplasmic reticulum; cytoplasm; nucleolus; extracellular region; IkappaB kinase complex; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding

Biological Process: positive regulation of apoptosis; apoptosis; myeloid dendritic cell activation during immune response; regulation of protein stability; positive regulation of caspase activity; positive regulation of JNK cascade; positive regulation of interleukin-1 beta secretion; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; signal transduction; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of adaptive immune response; caspase activation; interleukin-1 beta production; positive regulation of interleukin-6 production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; positive regulation of transcription factor activity; regulation of autophagy; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II
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 Research Articles on PYCARD    1. this study was to investigate the mRNA levels of AIM2 and ASC in a lymphocyte cell line (Jurkat) before and after MiR-143 introducing.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PYCARD elisa kitPathways associated with PYCARD elisa kit
 Reference Product  PubMed Publications
 CASP1 elisa kit  >120 publications with PYCARD and CASP1
 NLRP3 elisa kit  >79 publications with PYCARD and NLRP3
 NLRC4 elisa kit  >15 publications with PYCARD and NLRC4
 NLRP1 elisa kit  >8 publications with PYCARD and NLRP1
 MEFV elisa kit  >5 publications with PYCARD and MEFV
 POP1 elisa kit  >3 publications with PYCARD and POP1
 NLRP12 elisa kit  >1 publications with PYCARD and NLRP12
 Products by Pathway  Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
Diseases associated with PYCARD elisa kitOrgans/Tissues associated with PYCARD elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >81 publications with PYCARD and Inflammation
 Death Antibodies  >59 publications with PYCARD and Death
 Neoplasms Antibodies  >51 publications with PYCARD and Neoplasms
 Necrosis Antibodies  >40 publications with PYCARD and Necrosis
 Disease Models, Animal Antibodies  >15 publications with PYCARD and Disease Models, Animal
 Adenocarcinoma Antibodies  >11 publications with PYCARD and Adenocarcinoma
 Fever Antibodies  >10 publications with PYCARD and Fever
 Liver Diseases Antibodies  >8 publications with PYCARD and Liver Diseases
 Brain Diseases Antibodies  >7 publications with PYCARD and Brain Diseases
 Carcinoma, Small Cell Antibodies  >6 publications with PYCARD and Carcinoma, Small Cell
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >50 publications with PYCARD and Blood
 Bone Antibodies  >20 publications with PYCARD and Bone
 Lung Antibodies  >13 publications with PYCARD and Lung
 Liver Antibodies  >12 publications with PYCARD and Liver
 Brain Antibodies  >11 publications with PYCARD and Brain
 Kidney Antibodies  >8 publications with PYCARD and Kidney
 Skin Antibodies  >6 publications with PYCARD and Skin
 Intestine Antibodies  >6 publications with PYCARD and Intestine
 Vascular Antibodies  >5 publications with PYCARD and Vascular
 Lymph Antibodies  >4 publications with PYCARD and Lymph
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