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PALLD elisa kit :: Human Palladin (PALLD) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021828 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $355 +1 FREE 8GB USB
48-Strip-Wells  /  $540 +1 FREE 8GB USB
96-Strip-Wells  /  $730 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,985 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,410 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Palladin (PALLD), ELISA Kit

 Also Known As   

Palladin (PALLD) ELISA Kit

 Product Synonym Names    Cytoskeletal Associated Protein; Sarcoma antigen NY-SAR-77
 Product Gene Name   

PALLD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9ET54
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Palladin (PALLD).
No significant cross-reactivity or interference between Palladin (PALLD) and analogues was observed.
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 Samples    Tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Typically less than 0.051ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Palladin (PALLD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Palladin (PALLD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PALLD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PALLD purchase    MBS2021828 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Palladin (PALLD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing PALLD, with an estimated sensitivity of Typically less than 0.051ng/mL. The ELISA analytical biochemical technique of the MBS2021828 kit is based on PALLD antibody-PALLD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PALLD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PALLD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PALLD elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Palladin (PALLD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Palladin (PALLD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Palladin (PALLD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Palladin (PALLD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PALLD elisa kit    PALLD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2021828. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PALLD. It may not necessarily be applicable to this product.
 NCBI GI #    124487061
 NCBI GeneID    72333
 NCBI Accession #    NP_001074859.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001081390.1 [Other Products]
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 UniProt Primary Accession #    Q9ET54 [Other Products]
 UniProt Secondary Accession #    Q69ZT7; Q6DFX7; Q9CWW1; A0JNZ3 [Other Products]
 UniProt Related Accession #    Q9ET54 [Other Products]
 Molecular Weight    150,553 Da
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 NCBI Official Full Name    palladin isoform 4
 NCBI Official Synonym Full Names    palladin, cytoskeletal associated protein
 NCBI Official Symbol    Palld [Similar Products]
 NCBI Official Synonym Symbols   
6030492A02; 2410003B16Rik
[Similar Products]
 NCBI Protein Information    palladin
 UniProt Protein Name    Palladin
 Protein Family    Palladin
 UniProt Gene Name    Palld [Similar Products]
 UniProt Synonym Gene Names    Kiaa0992 [Similar Products]
 UniProt Entry Name    PALLD_MOUSE
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 UniProt Comments for PALLD    palladin: Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci. Genetic variations in PALLD are associated with susceptibility to pancreatic cancer type 1 (PNCA1). Expression is increased early in the development of pancreatic cancer: in normal-appearing whole tissue immediately adjacent to cancer, in the pre-cancer, and in both the familial and sporadic forms of the cancer. Genetic variations in PALLD may be associated with myocardial infarction. Belongs to the myotilin/palladin family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: focal adhesion; mitochondrion; actin filament; actin cytoskeleton; ruffle; cell projection; cytoskeleton; cell soma; axon; cytoplasm; plasma membrane; stress fiber; podosome; nucleus; cell junction; filopodium

Molecular Function: actin binding

Biological Process: ruffle organization and biogenesis; actin cytoskeleton organization and biogenesis; neurite development
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 Research Articles on PALLD    1. Suggesting that FSH signaling during Sertoli cell maturation regulates subcellular localization of palladin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PALLD elisa kitDiseases associated with PALLD elisa kit
 Products by Pathway  Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram
 Disease Name  Pubmed Publications
 Pancreatic Neoplasms Antibodies  >6 publications with PALLD and Pancreatic Neoplasms
 Heart Diseases Antibodies  >4 publications with PALLD and Heart Diseases
 Nervous System Diseases Antibodies  >4 publications with PALLD and Nervous System Diseases
 Breast Neoplasms Antibodies  >3 publications with PALLD and Breast Neoplasms
 Melanoma Antibodies  >3 publications with PALLD and Melanoma
 Myocardial Infarction Antibodies  >3 publications with PALLD and Myocardial Infarction
 Hypertension Antibodies  >2 publications with PALLD and Hypertension
 Precancerous Conditions Antibodies  >1 publications with PALLD and Precancerous Conditions
 Fatty Liver Antibodies  >1 publications with PALLD and Fatty Liver
 Liver Diseases Antibodies  >1 publications with PALLD and Liver Diseases
Organs/Tissues associated with PALLD elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with PALLD and Blood
 Liver Antibodies  >3 publications with PALLD and Liver
 Testis Antibodies  >2 publications with PALLD and Testis
 Heart Antibodies  >2 publications with PALLD and Heart
 Connective Tissue Antibodies  >1 publications with PALLD and Connective Tissue
 Lung Antibodies  >1 publications with PALLD and Lung
 Skin Antibodies  >1 publications with PALLD and Skin
 Pancreas Antibodies  >1 publications with PALLD and Pancreas
 Prostate Antibodies  >1 publications with PALLD and Prostate
 Embryonic Tissue Antibodies  >1 publications with PALLD and Embryonic Tissue
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