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PL elisa kit :: Human Pancreatic Lipase (PL) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS265974
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $420 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pancreatic Lipase (PL), ELISA Kit

 Also Known As   

Human Pancreatic Lipase (PL) ELISA Kit

 Product Gene Name   

PL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    246600
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PL concentration
 Assay Type    Sandwich
 Detection Range    20 ng/ml-0.312 ng/ml
 Sensitivity    0.06 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PL purchase    MBS265974 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pancreatic Lipase (PL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 20 ng/ml-0.312 ng/ml in biological research samples containing PL, with an estimated sensitivity of 0.06 ng/ml. The ELISA analytical biochemical technique of the MBS265974 kit is based on PL antibody-PL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PL concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PL elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of PL elisa kit    PL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS265974
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NCBI/Uniprot data below describe general gene information for PL. It may not necessarily be applicable to this product.
 NCBI GI #    1304379
 NCBI GeneID    5406
 NCBI Accession #    AAA99053.1 [Other Products]
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 UniProt Secondary Accession #    Q5VSQ2 [Other Products]
 UniProt Related Accession #    P16233 [Other Products]
 Molecular Weight    51,157 Da
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 NCBI Official Full Name    pancreatic lipase
 NCBI Official Synonym Full Names    pancreatic lipase
 NCBI Official Symbol    PNLIP [Similar Products]
 NCBI Official Synonym Symbols   
PL; PTL; PNLIPD
[Similar Products]
 NCBI Protein Information    pancreatic triacylglycerol lipase; triacylglycerol acylhydrolase
 UniProt Protein Name    Pancreatic triacylglycerol lipase
 UniProt Gene Name    PNLIP [Similar Products]
 UniProt Synonym Gene Names    PL; PTL; Pancreatic lipase [Similar Products]
 UniProt Entry Name    LIPP_HUMAN
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 NCBI Summary for PL    This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PL    PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Lipid Metabolism - glycerolipid; Hydrolase; Secreted, signal peptide; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: extracellular region

Molecular Function: triacylglycerol lipase activity; metal ion binding

Biological Process: phototransduction, visible light; cholesterol absorption; retinoid metabolic process; lipid catabolic process; lipid digestion

Disease: Pancreatic Lipase Deficiency
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 Research Articles on PL    1. Low PNLIP expression is associated with pancreatic cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PL elisa kitPathways associated with PL elisa kit
 Reference Product  PubMed Publications
 CLPS elisa kit  >2 publications with PL and CLPS
 CEL elisa kit  >1 publications with PL and CEL
 Products by Pathway  Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Acylglycerol Degradation Pathway antibodies  Acylglycerol Degradation Pathway Diagram
 Digestion Of Dietary Lipid Pathway antibodies  Digestion Of Dietary Lipid Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Glycerolipid Metabolism Pathway antibodies  Glycerolipid Metabolism Pathway Diagram
 Lipid Digestion, Mobilization, And Transport Pathway antibodies  Lipid Digestion, Mobilization, And Transport Pathway Diagram
Diseases associated with PL elisa kitOrgans/Tissues associated with PL elisa kit
 Disease Name  Pubmed Publications
 Pancreatitis Antibodies  >12 publications with PL and Pancreatitis
 Obesity Antibodies  >7 publications with PL and Obesity
 Weight Loss Antibodies  >4 publications with PL and Weight Loss
 Hyperlipidemias Antibodies  >2 publications with PL and Hyperlipidemias
 Insulin Resistance Antibodies  >2 publications with PL and Insulin Resistance
 Gastrointestinal Diseases Antibodies  >2 publications with PL and Gastrointestinal Diseases
 Diabetes Mellitus, Type 2 Antibodies  >2 publications with PL and Diabetes Mellitus, Type 2
 Hypertriglyceridemia Antibodies  >2 publications with PL and Hypertriglyceridemia
 Necrosis Antibodies  >2 publications with PL and Necrosis
 Kidney Diseases Antibodies  >1 publications with PL and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >23 publications with PL and Pancreas
 Liver Antibodies  >2 publications with PL and Liver
 Heart Antibodies  >1 publications with PL and Heart
 Adipose Tissue Antibodies  >1 publications with PL and Adipose Tissue
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