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PON1 elisa kit :: Human Paraoxonase 1 (PON1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2022720 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $255 +1 FREE 8GB USB
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $610 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Paraoxonase 1 (PON1), ELISA Kit

★Popular Item★
 Also Known As   

Paraoxonase 1 (PON1) ELISA Kit

 Product Synonym Names    ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
 Product Gene Name   

PON1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    168820
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PON1. No significant cross-reactivity or interference between PON1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    3.12-200ng/mL
 Sensitivity    1.27ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PON1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PON1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PON1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PON1 purchase    MBS2022720 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Paraoxonase 1 (PON1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12-200ng/mL in biological research samples containing PON1, with an estimated sensitivity of 1.27ng/mL. The ELISA analytical biochemical technique of the MBS2022720 kit is based on PON1 antibody-PON1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PON1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PON1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PON1 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PON1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PON1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of PON1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for PON1 elisa kit    Metabolic pathway; Endocrinology
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 Typical Testing Data/Standard Curve (for reference only) of PON1 elisa kit    PON1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2022720. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PON1. It may not necessarily be applicable to this product.
 NCBI GI #    345894407
 NCBI GeneID    5444
 NCBI Accession #    AEO20077.1 [Other Products]
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 UniProt Secondary Accession #    Q16052; Q6B0J6; Q9UCB1; B2RA40 [Other Products]
 UniProt Related Accession #    P27169 [Other Products]
 Molecular Weight    39,731 Da
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 NCBI Official Full Name    paraoxonase 1, partial
 NCBI Official Synonym Full Names    paraoxonase 1
 NCBI Official Symbol    PON1 [Similar Products]
 NCBI Official Synonym Symbols   
ESA; PON; MVCD5
[Similar Products]
 NCBI Protein Information    serum paraoxonase/arylesterase 1
 UniProt Protein Name    Serum paraoxonase/arylesterase 1
 UniProt Synonym Protein Names   
Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1
 Protein Family    Serum paraoxonase/arylesterase
 UniProt Gene Name    PON1 [Similar Products]
 UniProt Synonym Gene Names    PON; PON 1; A-esterase 1 [Similar Products]
 UniProt Entry Name    PON1_HUMAN
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 NCBI Summary for PON1    The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]
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 UniProt Comments for PON1    PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family.

Protein type: Motility/polarity/chemotaxis; EC 3.1.1.2; Lipid-binding; EC 3.1.8.1; Phosphatase (non-protein); Hydrolase; Secreted; Secreted, signal peptide; EC 3.1.1.81

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region

Molecular Function: protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity

Biological Process: response to external stimulus; response to nutrient levels; dephosphorylation; response to toxin; organophosphate catabolic process; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5
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 Research Articles on PON1    1. explore the physiological roles of PON1 in disease development, as well as PON1 gene and protein structure, promiscuous activities and the roles of SNPs and ethnicity in determining PON1 activity
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PON1 elisa kitPathways associated with PON1 elisa kit
 Reference Product  PubMed Publications
 APOE elisa kit  >82 publications with PON1 and APOE
 APOA1 elisa kit  >37 publications with PON1 and APOA1
 PPARG elisa kit  >18 publications with PON1 and PPARG
 CLU elisa kit  >11 publications with PON1 and CLU
 IL6 elisa kit  >5 publications with PON1 and IL6
 LPA elisa kit  >3 publications with PON1 and LPA
 PPARA elisa kit  >3 publications with PON1 and PPARA
 SREBF2 elisa kit  >2 publications with PON1 and SREBF2
 APOA2 elisa kit  >2 publications with PON1 and APOA2
 ALB elisa kit  >1 publications with PON1 and ALB
 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Phase I, Non P450 Pathway antibodies  Phase I, Non P450 Pathway Diagram
Diseases associated with PON1 elisa kitOrgans/Tissues associated with PON1 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >678 publications with PON1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >531 publications with PON1 and Atherosclerosis
 Coronary Disease Antibodies  >292 publications with PON1 and Coronary Disease
 Diabetes Mellitus Antibodies  >180 publications with PON1 and Diabetes Mellitus
 Inflammation Antibodies  >172 publications with PON1 and Inflammation
 Coronary Artery Disease Antibodies  >170 publications with PON1 and Coronary Artery Disease
 Kidney Diseases Antibodies  >86 publications with PON1 and Kidney Diseases
 Poisoning Antibodies  >85 publications with PON1 and Poisoning
 Organophosphate Poisoning Antibodies  >48 publications with PON1 and Organophosphate Poisoning
 Hypercholesterolemia Antibodies  >47 publications with PON1 and Hypercholesterolemia
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >193 publications with PON1 and Liver
 Brain Antibodies  >83 publications with PON1 and Brain
 Lung Antibodies  >49 publications with PON1 and Lung
 Bone Antibodies  >13 publications with PON1 and Bone
 Pancreas Antibodies  >8 publications with PON1 and Pancreas
 Testis Antibodies  >3 publications with PON1 and Testis
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