Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PGA5 rightarrow Human PGA5  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • TGR5 Inhibitor
 • Goat Anti-Mouse IgG3 (gamma 3 chain specific) Secondary Antibody
 • Lysozyme (Lyz) Antibody
 • Versican (VCAN) ELISA Kit
 • Angiotensin 1-9 (ANG1-9) ELISA Kit
 • Proteasome Subunit Beta Type 6 (PSMbeta6) ELISA Kit
 • Transferrin (TF) Antibody
 • FOLR1 Antibody
 • 3-phosphoinositide dependent protein kinase-1 (PDPK1) Antibody
 • Surfactant Associated Protein B (SPB) Recombinant Protein
 • TRK-fused gene (TFG) Antibody
 • T, brachyury homolog (mouse) ELISA Kit
 • BCL2 Associated X Protein (BAX) ELISA Kit
 • Testosterone (T) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PGA5 elisa kit :: Human Pepsin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS263717
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $420 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pepsin (PGA5), ELISA Kit

 Also Known As   

Human Pepsin ELISA Kit

 Product Gene Name   

PGA5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    gene 169730
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    No cross-reaction with other factors.
Section Bar
 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PP concentration
 Assay Type    Sandwich
 Detection Range    2000 pg/ml-31.2 pg/ml
 Sensitivity    12 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PGA5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PGA5 purchase    MBS263717 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pepsin (PGA5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2000 pg/ml-31.2 pg/ml in biological research samples containing PGA5, with an estimated sensitivity of 12 pg/ml. The ELISA analytical biochemical technique of the MBS263717 kit is based on PGA5 antibody-PGA5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PGA5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PGA5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PP concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for PGA5 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PGA5 elisa kit    PGA5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS263717
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PGA5. It may not necessarily be applicable to this product.
 NCBI GI #    23943854
 NCBI GeneID    5222
 NCBI Accession #    NP_055039.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_014224.3 [Other Products]
Section Bar
 UniProt Secondary Accession #    P00790; Q7M4R0; Q8N1E3; A8K749; B7ZW62; B7ZW75 [Other Products]
 UniProt Related Accession #    P0DJD9 [Other Products]
 Molecular Weight    41,993 Da [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    pepsin A-5 preproprotein
 NCBI Official Synonym Full Names    pepsinogen 5, group I (pepsinogen A)
 NCBI Official Symbol    PGA5 [Similar Products]
 NCBI Protein Information    pepsin A-5; pepsinogen-5; pepsinogen A5
 UniProt Protein Name    Pepsin A-5
 UniProt Synonym Protein Names   
Pepsinogen-5
 Protein Family    Pepsin
 UniProt Gene Name    PGA5 [Similar Products]
 UniProt Entry Name    PEPA5_HUMAN
Section Bar
 NCBI Summary for PGA5    This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
Section Bar
 UniProt Comments for PGA5    PGA5: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Belongs to the peptidase A1 family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.23.1

Chromosomal Location of Human Ortholog: 11q13

Molecular Function: aspartic-type endopeptidase activity

Biological Process: digestion; proteolysis
Table BarTOPTable Bar
 Research Articles on PGA5    1. Pepsin and pepsinogen in middle ear effusion are probably caused by LPR and may be involved in the pathogenesis of OME.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with PGA5 elisa kitOrgans/Tissues associated with PGA5 elisa kit
 Products by Pathway  Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Stomach Antibodies  >3 publications with PGA5 and Stomach
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions