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PI16 elisa kit :: Human Peptidase Inhibitor 16 (PI16) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024511 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $285 +1 FREE 8GB USB
48-Strip-Wells  /  $535 +1 FREE 8GB USB
96-Strip-Wells  /  $725 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peptidase Inhibitor 16 (PI16), ELISA Kit

★Popular Item★
 Also Known As   

ELISA Kit for Peptidase Inhibitor 16 (PI16)

 Product Gene Name   

PI16 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q6UXB8
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Peptidase Inhibitor 16 (PI16).
No significant cross-reactivity or interference between Peptidase Inhibitor 16 (PI16) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.064ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Peptidase Inhibitor 16 (PI16) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Peptidase Inhibitor 16 (PI16) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PI16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PI16 purchase    MBS2024511 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peptidase Inhibitor 16 (PI16) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing PI16, with an estimated sensitivity of < 0.064ng/mL. The ELISA analytical biochemical technique of the MBS2024511 kit is based on PI16 antibody-PI16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PI16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PI16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PI16 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidase Inhibitor 16 (PI16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidase Inhibitor 16 (PI16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidase Inhibitor 16 (PI16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidase Inhibitor 16 (PI16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for PI16 elisa kit    Enzyme & Kinase
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 Typical Testing Data/Standard Curve (for reference only) of PI16 elisa kit    PI16 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024511. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PI16. It may not necessarily be applicable to this product.
 NCBI GI #    312434019
 NCBI GeneID    221476
 NCBI Accession #    NP_001186088.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001199159.1 [Other Products]
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 UniProt Primary Accession #    Q6UXB8 [Other Products]
 UniProt Secondary Accession #    Q6ZVG9; Q8IYL8; Q8NBK0; Q8TCB8 [Other Products]
 UniProt Related Accession #    Q6UXB8 [Other Products]
 Molecular Weight    29,674 Da
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 NCBI Official Full Name    peptidase inhibitor 16
 NCBI Official Synonym Full Names    peptidase inhibitor 16
 NCBI Official Symbol    PI16 [Similar Products]
 NCBI Official Synonym Symbols   
PSPBP; CRISP9; MSMBBP
[Similar Products]
 NCBI Protein Information    peptidase inhibitor 16; PSP94-binding protein; cysteine-rich secretory protein 9; microseminoprotein, beta-binding protein; protease inhibitor 16
 UniProt Protein Name    Peptidase inhibitor 16
 UniProt Synonym Protein Names   
Cysteine-rich secretory protein 9; CRISP-9; PSP94-binding protein
 Protein Family    Peptidase inhibitor
 UniProt Gene Name    PI16 [Similar Products]
 UniProt Synonym Gene Names    CRISP9; PSPBP; PI-16; CRISP-9 [Similar Products]
 UniProt Entry Name    PI16_HUMAN
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 UniProt Comments for PI16    PI16: Putative serine protease inhibitor. Belongs to the CRISP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: extracellular space; integral to membrane

Molecular Function: protease inhibitor activity

Biological Process: negative regulation of peptidase activity
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 Research Articles on PI16    1. PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with PI16 elisa kitOrgans/Tissues associated with PI16 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >2 publications with PI16 and Disease Models, Animal
 Prostatic Diseases Antibodies  >2 publications with PI16 and Prostatic Diseases
 Neoplasms, Experimental Antibodies  >1 publications with PI16 and Neoplasms, Experimental
 Hypertrophy Antibodies  >1 publications with PI16 and Hypertrophy
 Fibrosis Antibodies  >1 publications with PI16 and Fibrosis
 Inflammation Antibodies  >1 publications with PI16 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4 publications with PI16 and Blood
 Prostate Antibodies  >3 publications with PI16 and Prostate
 Muscle Antibodies  >2 publications with PI16 and Muscle
 Heart Antibodies  >2 publications with PI16 and Heart
 Nerve Antibodies  >1 publications with PI16 and Nerve
 Ovary Antibodies  >1 publications with PI16 and Ovary
 Intestine Antibodies  >1 publications with PI16 and Intestine
 Kidney Antibodies  >1 publications with PI16 and Kidney
 Testis Antibodies  >1 publications with PI16 and Testis
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