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POSTN elisa kit :: Human Periostin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722502
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Periostin (POSTN), ELISA Kit

★Popular Item★
 Also Known As   

Human Periostin ELISA Kit

 Product Gene Name   

POSTN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608777
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of POSTN. No significant cross-reactivity or interference between POSTN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between POSTN and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of POSTN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for POSTN purchase    MBS722502 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Periostin (POSTN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing POSTN, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS722502 kit is based on POSTN antibody-POSTN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POSTN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POSTN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for POSTN elisa kit

   Intended Uses: This POSTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human POSTN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: POSTN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-POSTN antibody and an POSTN-HRP conjugate. The assay sample and buffer are incubated together with POSTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POSTN concentration since POSTN from samples and POSTN-HRP conjugate compete for the anti-POSTN antibody binding site. Since the number of sites is limited, as more sites are occupied by POSTN from the sample, fewer sites are left to bind POSTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The POSTN concentration in each sample is interpolated from this standard curve.
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  Typical Testing Data/Standard Curve (for reference only) of POSTN elisa kit    POSTN elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS722502. Click to request current manual
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NCBI/Uniprot data below describe general gene information for POSTN. It may not necessarily be applicable to this product.
 NCBI GI #    209862907
 NCBI GeneID    10631
 NCBI Accession #    NP_006466.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006475.2 [Other Products]
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 UniProt Secondary Accession #    Q15064; Q29XZ0; Q3KPJ5; Q5VSY5; Q8IZF9; B1ALD8; C0IMJ1; C0IMJ2; C0IMJ4; D2KRH7; F5H628 [Other Products]
 UniProt Related Accession #    Q15063 [Other Products]
 Molecular Weight    80,357 Da
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 NCBI Official Full Name    periostin isoform 1
 NCBI Official Synonym Full Names    periostin, osteoblast specific factor
 NCBI Official Symbol    POSTN [Similar Products]
 NCBI Official Synonym Symbols   
PN; OSF2; OSF-2; PDLPOSTN; periostin
[Similar Products]
 NCBI Protein Information    periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like)
 UniProt Protein Name    Periostin
 UniProt Synonym Protein Names   
Osteoblast-specific factor 2; OSF-2
 Protein Family    Periostin
 UniProt Gene Name    POSTN [Similar Products]
 UniProt Synonym Gene Names    OSF2; PN; OSF-2 [Similar Products]
 UniProt Entry Name    POSTN_HUMAN
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 NCBI Summary for POSTN    This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
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 UniProt Comments for POSTN    POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; trans-Golgi network

Molecular Function: heparin binding; protein binding; cell adhesion molecule binding

Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development
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 Research Articles on POSTN    1. Periostin plays an important role for proper collagen assembly and homeostasis. During skin aging periostin expression decreases and contributes to the phenotype of aged skin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with POSTN elisa kitDiseases associated with POSTN elisa kit
 Reference Product  PubMed Publications
 TGFB1 elisa kit  >30 publications with POSTN and TGFB1
 COL1A1 elisa kit  >7 publications with POSTN and COL1A1
 COL3A1 elisa kit  >7 publications with POSTN and COL3A1
 DCN elisa kit  >6 publications with POSTN and DCN
 COL1A2 elisa kit  >5 publications with POSTN and COL1A2
 FN1 elisa kit  >5 publications with POSTN and FN1
 CLCA1 elisa kit  >4 publications with POSTN and CLCA1
 SERPINB2 elisa kit  >4 publications with POSTN and SERPINB2
 COL5A2 elisa kit  >3 publications with POSTN and COL5A2
 LUM elisa kit  >3 publications with POSTN and LUM
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >60 publications with POSTN and Fibrosis
 Inflammation Antibodies  >44 publications with POSTN and Inflammation
 Neoplasm Metastasis Antibodies  >37 publications with POSTN and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >37 publications with POSTN and Disease Models, Animal
 Adenocarcinoma Antibodies  >29 publications with POSTN and Adenocarcinoma
 Lung Neoplasms Antibodies  >25 publications with POSTN and Lung Neoplasms
 Breast Neoplasms Antibodies  >25 publications with POSTN and Breast Neoplasms
 Myocardial Infarction Antibodies  >19 publications with POSTN and Myocardial Infarction
 Heart Failure Antibodies  >18 publications with POSTN and Heart Failure
 Nervous System Diseases Antibodies  >14 publications with POSTN and Nervous System Diseases
Organs/Tissues associated with POSTN elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >112 publications with POSTN and Bone
 Muscle Antibodies  >72 publications with POSTN and Muscle
 Heart Antibodies  >67 publications with POSTN and Heart
 Connective Tissue Antibodies  >60 publications with POSTN and Connective Tissue
 Lung Antibodies  >53 publications with POSTN and Lung
 Vascular Antibodies  >48 publications with POSTN and Vascular
 Mouth Antibodies  >46 publications with POSTN and Mouth
 Embryonic Tissue Antibodies  >35 publications with POSTN and Embryonic Tissue
 Skin Antibodies  >30 publications with POSTN and Skin
 Liver Antibodies  >21 publications with POSTN and Liver
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