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PPAR-gamma elisa kit :: Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS263089
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $420 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma), ELISA Kit

 Also Known As   

Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit

 Product Synonym Names    Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-g)  ELISA Kit
 Product Gene Name   

PPAR-gamma elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS263089 COA    COA PDF
 OMIM    609338
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 Species Reactivity    Human
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 Specificity    No cross-reaction with other factors.
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 Samples    Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PPAR-gamma concentration
 Assay Type    Sandwich
 Detection Range    50 ng/ml-0.78ng/ml
 Sensitivity    0.08ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PPAR-gamma elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PPAR-gamma purchase    MBS263089 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50 ng/ml-0.78ng/ml in biological research samples containing PPAR-gamma, with an estimated sensitivity of 0.08ng/ml. The ELISA analytical biochemical technique of the MBS263089 kit is based on PPAR-gamma antibody-PPAR-gamma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPAR-gamma antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPAR-gamma. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PPAR-gamma concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PPAR-gamma elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PPAR-gamma monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of PPAR-gamma elisa kit    PPAR-gamma elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS263089
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NCBI/Uniprot data below describe general gene information for PPAR-gamma. It may not necessarily be applicable to this product.
 NCBI GI #    17978516
 NCBI Accession #    AAA80314.2 [Other Products]
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 UniProt Secondary Accession #    O00684; O00710; O14515; Q0QJH8; Q15178; Q15179; Q15180; Q15832; Q86U60; A8K3G6; B5BUA1 [Other Products]
 Molecular Weight    21,580 Da
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 NCBI Official Full Name    peroxisome proliferator activated receptor gamma
 UniProt Protein Name    Peroxisome proliferator-activated receptor gamma
 UniProt Synonym Protein Names   
Nuclear receptor subfamily 1 group C member 3
 UniProt Gene Name    PPARG [Similar Products]
 UniProt Synonym Gene Names    NR1C3; PPAR-gamma [Similar Products]
 UniProt Entry Name    PPARG_HUMAN
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 UniProt Comments for PPAR-gamma    PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol

Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; arachidonic acid binding; retinoid X receptor binding; protein binding; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity

Biological Process: heart development; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; cell maturation; rhythmic process; lipid homeostasis; glucose homeostasis; response to lipid; response to vitamin A; response to caffeine; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to estrogen stimulus; response to mechanical stimulus; lipoprotein transport; brown fat cell differentiation; positive regulation of fat cell differentiation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; positive regulation of transcription factor activity; positive regulation of phagocytosis, engulfment; negative regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; positive regulation of fatty acid oxidation; white fat cell differentiation; innate immune response; gene expression; response to cold; negative regulation of cell growth; lipid metabolic process

Disease: Obesity; Carotid Intimal Medial Thickness 1; Lipodystrophy, Familial Partial, Type 3; Diabetes Mellitus, Noninsulin-dependent
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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