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PIK3R6 elisa kit :: Human Phosphoinositide 3-kinase regulatory subunit 6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS280820 PIK3R6 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
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 Product Name   

Phosphoinositide 3-kinase regulatory subunit 6 (PIK3R6), ELISA Kit

 Also Known As   

Human Phosphoinositide 3-kinase regulatory subunit 6 (PIK3R6) ELISA Kit

 Product Gene Name   

PIK3R6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    AY753192 mRNA
 3D Structure    ModBase 3D Structure for Q5UE93
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PIK3R6. No significant cross-reactivity or interference between Human PIK3R6 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PIK3R6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PIK3R6 purchase    MBS280820 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphoinositide 3-kinase regulatory subunit 6 (PIK3R6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PIK3R6. The ELISA analytical biochemical technique of the MBS280820 kit is based on PIK3R6 antibody-PIK3R6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIK3R6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIK3R6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PIK3R6 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIK3R6 in samples. An antibody specific for PIK3R6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3R6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3R6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3R6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for PIK3R6. It may not necessarily be applicable to this product.
 NCBI GI #    58082081
 NCBI GeneID    146850
 NCBI Accession #    NP_001010855.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001010855.3 [Other Products]
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 UniProt Primary Accession #    Q5UE93 [Other Products]
 UniProt Secondary Accession #    Q658R3 [Other Products]
 UniProt Related Accession #    Q5UE93 [Other Products]
 Molecular Weight    84,258 Da
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 NCBI Official Full Name    phosphoinositide 3-kinase regulatory subunit 6 isoform 1
 NCBI Official Synonym Full Names    phosphoinositide-3-kinase regulatory subunit 6
 NCBI Official Symbol    PIK3R6 [Similar Products]
 NCBI Official Synonym Symbols   
C17orf38; HsT41028; p87PIKAP; p84 PIKAP; p87(PIKAP)
[Similar Products]
 NCBI Protein Information    phosphoinositide 3-kinase regulatory subunit 6
 UniProt Protein Name    Phosphoinositide 3-kinase regulatory subunit 6
 UniProt Synonym Protein Names   
Phosphoinositide 3-kinase gamma adapter protein of 87 kDa; p84 PI3K adapter protein; p84 PIKAP; p87 PI3K adapter protein; p87PIKAP
 Protein Family    Phosphoinositide 3-kinase regulatory
 UniProt Gene Name    PIK3R6 [Similar Products]
 UniProt Synonym Gene Names    C17orf38; p84 PIKAP; p87PIKAP [Similar Products]
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 NCBI Summary for PIK3R6    Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 7. The orthologous protein in the mouse binds to both the catalytic subunit and to G(beta/gamma), and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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 UniProt Comments for PIK3R6    PIK3R6: Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G protein dimers than PIK3CG:PIK3R5 and its membrane recruitment and beta-gamma G protein dimer-dependent activation requires HRAS1 bound to PIK3CG. Recruits of the PI3K gamma complex to a PDE3B:RAPGEF3 signaling complex involved in angiogenesis; signaling seems to involve RRAS.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytosol; membrane; phosphoinositide 3-kinase complex; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase regulator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding

Biological Process: angiogenesis; G-protein coupled receptor signaling pathway; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade; platelet activation; positive regulation of angiogenesis; positive regulation of MAP kinase activity; positive regulation of T cell differentiation; regulation of natural killer cell mediated cytotoxicity; regulation of phosphoinositide 3-kinase activity
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 Research Articles on PIK3R6    1. Our findings suggest that p84 binding to p110gamma may represent a novel negative feedback signal that terminates PI3Kgamma activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with PIK3R6 elisa kit
 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 G Beta:gamma Signalling Through PI3Kgamma Pathway antibodies  G Beta:gamma Signalling Through PI3Kgamma Pathway Diagram
 G-protein Beta:gamma Signalling Pathway antibodies  G-protein Beta:gamma Signalling Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPVI-mediated Activation Cascade Pathway antibodies  GPVI-mediated Activation Cascade Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 PI Metabolism Pathway antibodies  PI Metabolism Pathway Diagram
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