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LpPLA2 elisa kit :: Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2702623
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $210 +1 FREE 8GB USB
48-Strip-Wells  /  $330 +1 FREE 8GB USB
96-Strip-Wells  /  $440 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,820 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,365 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phospholipase A2, Lipoprotein Associated (LpPLA2), ELISA Kit

★Popular Item★
 Also Known As   

Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

 Product Synonym Names    PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2
 Product Gene Name   

LpPLA2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    M22430 mRNA
 3D Structure    ModBase 3D Structure for P14555
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Lipoprotein Associated (LpPLA2).
No significant cross-reactivity or interference between Phospholipase A2, Lipoprotein Associated (LpPLA2) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    0.625-40ng/mL
 Sensitivity    < 0.286ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LpPLA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LpPLA2 purchase    MBS2702623 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625-40ng/mL in biological research samples containing LpPLA2, with an estimated sensitivity of < 0.286ng/mL. The ELISA analytical biochemical technique of the MBS2702623 kit is based on LpPLA2 antibody-LpPLA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LpPLA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LpPLA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LpPLA2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Lipoprotein Associated (LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Lipoprotein Associated (LpPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of LpPLA2 elisa kit    LpPLA2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2702623. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LpPLA2. It may not necessarily be applicable to this product.
 NCBI GI #    239915991
 NCBI GeneID    5320
 NCBI Accession #    NP_001155201.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001161729.1 [Other Products]
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 UniProt Primary Accession #    P14555 [Other Products]
 UniProt Secondary Accession #    Q6DN24; Q6IBD9; Q9UCD2; A8K5I7 [Other Products]
 UniProt Related Accession #    P14555 [Other Products]
 Molecular Weight    16,083 Da
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 NCBI Official Full Name    phospholipase A2, membrane associated
 NCBI Official Synonym Full Names    phospholipase A2 group IIA
 NCBI Official Symbol    PLA2G2A [Similar Products]
 NCBI Official Synonym Symbols   
MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
[Similar Products]
 NCBI Protein Information    phospholipase A2, membrane associated
 UniProt Protein Name    Phospholipase A2, membrane associated
 UniProt Synonym Protein Names   
GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A
 UniProt Gene Name    PLA2G2A [Similar Products]
 UniProt Synonym Gene Names    PLA2B; PLA2L; RASF-A; NPS-PLA2 [Similar Products]
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 NCBI Summary for LpPLA2    The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]
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 UniProt Comments for LpPLA2    Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:2925633). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins (PubMed:18635536, PubMed:25398877). Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 (PubMed:18635536, PubMed:25398877). Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:25398877). Induces cell proliferation in an integrin-dependent manner (PubMed:18635536).
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 Research Articles on LpPLA2    1. the activity of human cPLA2alpha towards a multitude of glycerophospholipids species present in micelles or bilayers, was investigated.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LpPLA2 elisa kitPathways associated with LpPLA2 elisa kit
 Reference Product  PubMed Publications
 PTGS2 elisa kit  >5 publications with LpPLA2 and PTGS2
 PTGS1 elisa kit  >2 publications with LpPLA2 and PTGS1
 PLA2G7 elisa kit  >1 publications with LpPLA2 and PLA2G7
 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Acyl Chain Remodelling Of PI Pathway antibodies  Acyl Chain Remodelling Of PI Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
Diseases associated with LpPLA2 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >33 publications with LpPLA2 and Inflammation
 Colorectal Neoplasms Antibodies  >25 publications with LpPLA2 and Colorectal Neoplasms
 Colonic Neoplasms Antibodies  >19 publications with LpPLA2 and Colonic Neoplasms
 Heart Diseases Antibodies  >8 publications with LpPLA2 and Heart Diseases
 Atherosclerosis Antibodies  >8 publications with LpPLA2 and Atherosclerosis
 Lung Neoplasms Antibodies  >6 publications with LpPLA2 and Lung Neoplasms
 Necrosis Antibodies  >5 publications with LpPLA2 and Necrosis
 Myocardial Ischemia Antibodies  >5 publications with LpPLA2 and Myocardial Ischemia
 Liver Diseases Antibodies  >4 publications with LpPLA2 and Liver Diseases
 Hyperplasia Antibodies  >4 publications with LpPLA2 and Hyperplasia
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