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PLB1 elisa kit :: Human Phospholipase B1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS280515 PLB1 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Phospholipase B1 (PLB1), ELISA Kit

★Popular Item★
 Also Known As   

Human Phospholipase B1, membrane-associated (PLB1) ELISA Kit

 Product Gene Name   

PLB1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    AC074011 Genomic DNA
 3D Structure    ModBase 3D Structure for Q6P1J6
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PLB1. No significant cross-reactivity or interference between Human PLB1 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PLB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PLB1 purchase    MBS280515 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipase B1 (PLB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLB1. The ELISA analytical biochemical technique of the MBS280515 kit is based on PLB1 antibody-PLB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PLB1 elisa kit

   By Western blot analysis using an antibody against guinea pig phospholipase B (PLB), Maury et al. (2002) detected a 97-kD polypeptide in the soluble and membrane fractions of human epidermis that was enriched in the soluble fraction. Consistent with these results, they found that PLB activity was greatest in the soluble fraction.
Trypsin had no effect on the molecular mass or enzymatic activity, suggesting that the protein they detected was already in an active form.
Immunolocalization experiments revealed that PLB is expressed in the entire human epidermis with an accumulation in the dermoepidermis junction. In situ hybridization also indicated expression in the entire epidermis.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLB1 in samples. An antibody specific for PLB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for PLB1. It may not necessarily be applicable to this product.
 NCBI GI #    283436114
 NCBI GeneID    151056
 NCBI Accession #    NP_001164056.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001170585.1 [Other Products]
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 UniProt Primary Accession #    Q6P1J6 [Other Products]
 UniProt Secondary Accession #    Q53S03; Q8IUP7; Q96DP9; A8KAX2 [Other Products]
 UniProt Related Accession #    Q6P1J6 [Other Products]
 Molecular Weight    47,341 Da
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 NCBI Official Full Name    phospholipase B1, membrane-associated isoform 2
 NCBI Official Synonym Full Names    phospholipase B1
 NCBI Official Symbol    PLB1 [Similar Products]
 NCBI Official Synonym Symbols   
PLB; PLB/LIP
[Similar Products]
 NCBI Protein Information    phospholipase B1, membrane-associated
 UniProt Protein Name    Phospholipase B1, membrane-associated
 UniProt Synonym Protein Names   
Phospholipase B/lipase; PLB/LIP
 Protein Family    Phospholipase
 UniProt Gene Name    PLB1 [Similar Products]
 UniProt Synonym Gene Names    PLB; Phospholipase B; hPLB; PLB/LIP [Similar Products]
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 NCBI Summary for PLB1    This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene. [provided by RefSeq, Jul 2016]
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 UniProt Comments for PLB1    PLB1: Membrane-associated phospholipase. Exhibits a calcium- independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids. Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.4; EC 3.1.1.5; Membrane protein, integral; Phospholipase

Chromosomal Location of Human Ortholog: 2p23.2

Cellular Component: apical plasma membrane; integral component of membrane; plasma membrane

Molecular Function: lysophospholipase activity; phospholipase A2 activity; retinyl-palmitate esterase activity

Biological Process: lipid catabolic process; retinoid metabolic process; retinol metabolic process
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 Research Articles on PLB1    1. rs117512489 in gene phospholipase B1 gene is associated with Non-Small Cell Lung Cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PLB1 elisa kitPathways associated with PLB1 elisa kit
 Reference Product  PubMed Publications
 PLA2G4A elisa kit  >2 publications with PLB1 and PLA2G4A
 PLD1 elisa kit  >1 publications with PLB1 and PLD1
 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram
Diseases associated with PLB1 elisa kitOrgans/Tissues associated with PLB1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with PLB1 and Inflammation
 Breast Neoplasms Antibodies  >1 publications with PLB1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with PLB1 and Neoplasms, Experimental
 Carcinoma Antibodies  >1 publications with PLB1 and Carcinoma
 Necrosis Antibodies  >1 publications with PLB1 and Necrosis
 Arthritis, Rheumatoid Antibodies  >1 publications with PLB1 and Arthritis, Rheumatoid
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with PLB1 and Blood
 Brain Antibodies  >7 publications with PLB1 and Brain
 Lung Antibodies  >6 publications with PLB1 and Lung
 Kidney Antibodies  >1 publications with PLB1 and Kidney
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