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EGFLAM elisa kit :: Human Pikachurin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS760605
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pikachurin (EGFLAM), ELISA Kit

★Popular Item★
 Also Known As   

Human Pikachurin ELISA Kit

 Product Synonym Names    EGFLAM/Agrin-like protein/EGF-like; fibronectin type-III and laminin G-like domain-containing protein/AGRINL/AGRNL
 Product Gene Name   

EGFLAM elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS760605 COA    COA PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of EGFLAM. No significant cross-reactivity or interference between EGFLAM and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.312-20ng/ml
 Sensitivity    0.188ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGFLAM were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGFLAM were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of EGFLAM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for EGFLAM purchase    MBS760605 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pikachurin (EGFLAM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/ml in biological research samples containing EGFLAM, with an estimated sensitivity of 0.188ng/ml. The ELISA analytical biochemical technique of the MBS760605 kit is based on EGFLAM antibody-EGFLAM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EGFLAM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EGFLAM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for EGFLAM elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- EGFLAM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- EGFLAM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EGFLAM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EGFLAM can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of EGFLAM elisa kit    EGFLAM elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS760605. Click to request current manual
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NCBI/Uniprot data below describe general gene information for EGFLAM. It may not necessarily be applicable to this product.
 NCBI GI #    328927010
 NCBI GeneID    133584
 NCBI Accession #    NP_001192230.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001205301.1 [Other Products]
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 UniProt Secondary Accession #    Q5U643; Q6P3V1; Q8N124; Q8N197; Q8N7Y0; Q8N8N5; Q8NAL2; A8K6D7 [Other Products]
 UniProt Related Accession #    Q63HQ2 [Other Products]
 Molecular Weight    16,588 Da
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 NCBI Official Full Name    pikachurin isoform 5
 NCBI Official Synonym Full Names    EGF like, fibronectin type III and laminin G domains
 NCBI Official Symbol    EGFLAM [Similar Products]
 NCBI Official Synonym Symbols   
PIKA; AGRNL; AGRINL
[Similar Products]
 NCBI Protein Information    pikachurin
 UniProt Protein Name    Pikachurin
 UniProt Synonym Protein Names   
Agrin-like protein; EGF-like, fibronectin type-III and laminin G-like domain-containing protein
 Protein Family    Pikachurin
 UniProt Gene Name    EGFLAM [Similar Products]
 UniProt Synonym Gene Names    AGRINL; AGRNL [Similar Products]
 UniProt Entry Name    EGFLA_HUMAN
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 UniProt Comments for EGFLAM    EGFLAM: Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13.2-p13.1

Cellular Component: basement membrane; cell junction; synapse

Molecular Function: glycosaminoglycan binding

Biological Process: extracellular matrix organization and biogenesis; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan
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 Research Articles on EGFLAM    1. Strong candidate gene for macular dystrophy, MCDR3 (human 5p15.33-p13.1). Conclusion is based on a massive expression data set for mouse (103 strains) and joint analysis of RetNet database.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with EGFLAM elisa kitOrgans/Tissues associated with EGFLAM elisa kit
 Disease Name  Pubmed Publications
 Ovarian Diseases Antibodies  >1 publications with EGFLAM and Ovarian Diseases
 Nervous System Diseases Antibodies  >1 publications with EGFLAM and Nervous System Diseases
 Nervous System Malformations Antibodies  >1 publications with EGFLAM and Nervous System Malformations
 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >1 publications with EGFLAM and Ovary
 Brain Antibodies  >1 publications with EGFLAM and Brain
 Placenta Antibodies  >1 publications with EGFLAM and Placenta
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