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PIM1 elisa kit :: Human Pim-1 Oncogene (PIM1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019381 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pim-1 Oncogene (PIM1), ELISA Kit

★Popular Item★
 Also Known As   

Pim-1 Oncogene (PIM1) ELISA Kit

 Product Synonym Names    Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1
 Product Gene Name   

PIM1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    164960
 3D Structure    ModBase 3D Structure for P11309
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PIM1. No significant cross-reactivity or interference between PIM1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between PIM1 and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.78-50ng/mL
 Sensitivity    0.26ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PIM1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PIM1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PIM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PIM1 purchase    MBS2019381 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pim-1 Oncogene (PIM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.78-50ng/mL in biological research samples containing PIM1, with an estimated sensitivity of 0.26ng/mL. The ELISA analytical biochemical technique of the MBS2019381 kit is based on PIM1 antibody-PIM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PIM1 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIM1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PIM1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PIM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of PIM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for PIM1 elisa kit    Tumor immunity
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 Typical Testing Data/Standard Curve (for reference only) of PIM1 elisa kit    PIM1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019381. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PIM1. It may not necessarily be applicable to this product.
 NCBI GI #    18044378
 NCBI GeneID    5292
 NCBI Accession #    AAH20224.1 [Other Products]
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 UniProt Primary Accession #    P11309 [Other Products]
 UniProt Secondary Accession #    Q38RT9; Q5T7H7; Q96RG3 [Other Products]
 UniProt Related Accession #    P11309 [Other Products]
 Molecular Weight    35,686 Da
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 NCBI Official Full Name    Pim-1 oncogene
 NCBI Official Synonym Full Names    Pim-1 proto-oncogene, serine/threonine kinase
 NCBI Official Symbol    PIM1 [Similar Products]
 NCBI Official Synonym Symbols   
PIM
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase pim-1
 UniProt Protein Name    Serine/threonine-protein kinase pim-1
 Protein Family    Protein
 UniProt Gene Name    PIM1 [Similar Products]
 UniProt Entry Name    PIM1_HUMAN
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 NCBI Summary for PIM1    The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]
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 UniProt Comments for PIM1    Pim1: a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of ASK1 an other proapoptotic protein, by PIM1, significantly decreases ASK1 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of p21Cip1, a regulator of cell cycle progression at G1, results in the relocation of p21Cip1 to the cytoplasm and enhanced p21Cip1 protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, p27Kip1, at both transcriptional and post- translational levels. Phosphorylation of p27Kip1,induces 14-3-3- proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with p27Kip1 and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Ubiquitinated form interacts with HSP70 and promotes its proteosomal degradation. Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines. Induced by different cellular stresses, heat shock and cytotoxic agents. Expressed primarily in cells of the hematopoietic and germline lineages. 2 isoforms of the human protein are produced by alternative initiation. Both isoforms are expressed in prostate cancer cell lines.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; CAMK group; PIM family

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; manganese ion binding; transcription factor binding; ATP binding; ribosomal small subunit binding

Biological Process: cell proliferation; apoptosis; negative regulation of transcription factor activity; multicellular organismal development; protein amino acid autophosphorylation; regulation of mitotic cell cycle; cell cycle; protein amino acid phosphorylation; hyaluronan metabolic process; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; negative regulation of apoptosis
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 Research Articles on PIM1    1. These results point on PIM1 as a novel factor in regulation of the phenotype and differentiation of fibroblasts in prostate cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PIM1 elisa kitPathways associated with PIM1 elisa kit
 Reference Product  PubMed Publications
 FLT3 elisa kit  >16 publications with PIM1 and FLT3
 PRL elisa kit  >7 publications with PIM1 and PRL
 CDKN1A elisa kit  >7 publications with PIM1 and CDKN1A
 CXCR4 elisa kit  >6 publications with PIM1 and CXCR4
 CDC25A elisa kit  >4 publications with PIM1 and CDC25A
 HOXA9 elisa kit  >3 publications with PIM1 and HOXA9
 RUNX1 elisa kit  >1 publications with PIM1 and RUNX1
 SNX6 elisa kit  >1 publications with PIM1 and SNX6
 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 IL-5 Signaling Pathway antibodies  IL-5 Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway antibodies  Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Activation Pathway antibodies  Validated Targets Of C-MYC Transcriptional Activation Pathway Diagram
Diseases associated with PIM1 elisa kitOrgans/Tissues associated with PIM1 elisa kit
 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >36 publications with PIM1 and Cell Transformation, Neoplastic
 Disease Models, Animal Antibodies  >25 publications with PIM1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >23 publications with PIM1 and Neoplasms, Experimental
 Inflammation Antibodies  >18 publications with PIM1 and Inflammation
 Heart Diseases Antibodies  >16 publications with PIM1 and Heart Diseases
 Liver Diseases Antibodies  >11 publications with PIM1 and Liver Diseases
 Adenocarcinoma Antibodies  >10 publications with PIM1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >7 publications with PIM1 and Breast Neoplasms
 Hyperplasia Antibodies  >6 publications with PIM1 and Hyperplasia
 Necrosis Antibodies  >6 publications with PIM1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >113 publications with PIM1 and Blood
 Prostate Antibodies  >43 publications with PIM1 and Prostate
 Bone Antibodies  >33 publications with PIM1 and Bone
 Muscle Antibodies  >31 publications with PIM1 and Muscle
 Heart Antibodies  >27 publications with PIM1 and Heart
 Bone Marrow Antibodies  >26 publications with PIM1 and Bone Marrow
 Liver Antibodies  >21 publications with PIM1 and Liver
 Vascular Antibodies  >14 publications with PIM1 and Vascular
 Lung Antibodies  >14 publications with PIM1 and Lung
 Brain Antibodies  >12 publications with PIM1 and Brain
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