Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PITX3 rightarrow Human PITX3  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • NAD (P) transhydrogenase subunit beta (pntB) Recombinant Protein
 • Chorionic Gonadotropin, Equine (CYP19A1) Antibody
 • family with sequence similarity 19 (chemokine (C-C motif) -l... ELISA Kit
 • neuronal nuclear autoantibody, ANNA-1-Hu (Anxa1) ELISA Kit
 • Glucagon Like Peptide 2 (GLP2) ELISA Kit
 • Fibroblast growth factor 23 protein (FGF23) Recombinant Protein
 • Progonadoliberin-1 (Gnrh1) ELISA Kit
 • Interleukin 33 (IL-33) ELISA Kit
 • Resolvin E1 (RvE1 ) ELISA Kit
 • Kafirin PSK8 Recombinant Protein
 • Human Chorionic Gonadotropin (hCG) >98% Pure Native Protein
 • TSLP ELISA Kit
 • Artemin ELISA Kit
 • Cotinine-4-HRP Conjugate Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PITX3 elisa kit :: Human Pituitary homeobox 3 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS282545 PITX3 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pituitary homeobox 3 (PITX3), ELISA Kit

 Also Known As   

Human Pituitary homeobox 3 (PITX3) ELISA Kit

 Product Gene Name   

PITX3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Request for Current Manual Insert    Request Current Manual
 OMIM    AF041339 mRNA
 3D Structure    ModBase 3D Structure for O75364
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PITX3. No significant cross-reactivity or interference between Human PITX3 and analogues was observed.
Section Bar
 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
Section Bar
 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PITX3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PITX3 purchase    MBS282545 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pituitary homeobox 3 (PITX3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PITX3. The ELISA analytical biochemical technique of the MBS282545 kit is based on PITX3 antibody-PITX3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PITX3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PITX3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for PITX3 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PITX3 in samples. An antibody specific for PITX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPITX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PITX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PITX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for PITX3. It may not necessarily be applicable to this product.
 NCBI GI #    4826912
 NCBI GeneID    5309
 NCBI Accession #    NP_005020.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005029.3 [Other Products]
Section Bar
 UniProt Primary Accession #    O75364 [Other Products]
 UniProt Secondary Accession #    Q5VZL2 [Other Products]
 UniProt Related Accession #    O75364 [Other Products]
 Molecular Weight    31,832 Da
Table BarTOPTable Bar
 NCBI Official Full Name    pituitary homeobox 3
 NCBI Official Synonym Full Names    paired like homeodomain 3
 NCBI Official Symbol    PITX3 [Similar Products]
 NCBI Official Synonym Symbols   
ASMD; ASOD; PTX3; ASGD1; CTPP4; CTRCT11
[Similar Products]
 NCBI Protein Information    pituitary homeobox 3
 UniProt Protein Name    Pituitary homeobox 3
 UniProt Synonym Protein Names   
Homeobox protein PITX3; Paired-like homeodomain transcription factor 3
 Protein Family    Pituitary homeobox
 UniProt Gene Name    PITX3 [Similar Products]
 UniProt Synonym Gene Names    PTX3 [Similar Products]
Section Bar
 NCBI Summary for PITX3    This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for PITX3    PITX3: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1- mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle. Defects in PITX3 are a cause of cataract autosomal dominant (ADC). Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood. Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD); also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis. Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4). A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: cell soma; nucleus

Biological Process: aging; dopaminergic neuron differentiation; lens development in camera-type eye; lens fiber cell differentiation; lens morphogenesis in camera-type eye; locomotory behavior; midbrain development; negative regulation of gliogenesis; neuron development; organ morphogenesis; positive regulation of cell proliferation in midbrain; positive regulation of neuron apoptotic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; response to cocaine; response to methamphetamine hydrochloride; response to morphine; transcription from RNA polymerase II promoter

Disease: Anterior Segment Mesenchymal Dysgenesis; Cataract 11, Multiple Types
Table BarTOPTable Bar
 Research Articles on PITX3    1. Study showed that PITX3 methylation was significantly methylated in head and neck squamous cell carcinoma (HNSCC) tumor compared to normal adjacent tissue and correlated with lymph node status. These results provide evidence that PITX3 DNA methylation is an independent prognostic biomarker for overall survival in patients with HNSCC and might aid in the process of risk stratification for individualized treatment.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PITX3 elisa kitDiseases associated with PITX3 elisa kit
 Reference Product  PubMed Publications
 FOXE3 elisa kit  >11 publications with PITX3 and FOXE3
 BDNF elisa kit  >7 publications with PITX3 and BDNF
 CRYBB1 elisa kit  >3 publications with PITX3 and CRYBB1
 BFSP2 elisa kit  >2 publications with PITX3 and BFSP2
 CRYBB3 elisa kit  >2 publications with PITX3 and CRYBB3
 MTA1 elisa kit  >1 publications with PITX3 and MTA1
 CHMP4B elisa kit  >1 publications with PITX3 and CHMP4B
 PARK7 elisa kit  >1 publications with PITX3 and PARK7
 Disease Name  Pubmed Publications
 Movement Disorders Antibodies  >64 publications with PITX3 and Movement Disorders
 Disease Models, Animal Antibodies  >31 publications with PITX3 and Disease Models, Animal
 Eye Abnormalities Antibodies  >28 publications with PITX3 and Eye Abnormalities
 Cataract Antibodies  >27 publications with PITX3 and Cataract
 Neurologic Manifestations Antibodies  >13 publications with PITX3 and Neurologic Manifestations
 Embryo Loss Antibodies  >11 publications with PITX3 and Embryo Loss
 Heart Defects, Congenital Antibodies  >10 publications with PITX3 and Heart Defects, Congenital
 Microphthalmos Antibodies  >6 publications with PITX3 and Microphthalmos
 Anterior segment mesenchymal dysgenesis Antibodies  >6 publications with PITX3 and Anterior segment mesenchymal dysgenesis
 Neurobehavioral Manifestations Antibodies  >5 publications with PITX3 and Neurobehavioral Manifestations
Organs/Tissues associated with PITX3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >65 publications with PITX3 and Eye
 Muscle Antibodies  >15 publications with PITX3 and Muscle
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions