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PLGF elisa kit :: Human Placenta Growth Factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS720145
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Placenta Growth Factor (PLGF), ELISA Kit

★Popular Item★
 Also Known As   

Human Placenta Growth Factor ELISA Kit

 Product Gene Name   

PLGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PLGF. No significant cross-reactivity or interference between PLGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PLGF and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
 Intended Uses    This PLGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PLGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PLGF purchase    MBS720145 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Placenta Growth Factor (PLGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing PLGF, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS720145 kit is based on PLGF antibody-PLGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PLGF elisa kit

   Princple of the assay: PLGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLGF. Standards or samples are then added to the microtiter plate wells and PLGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PLGF are added to each well to “sandwich” the PLGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLGF concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PLGF elisa kit    PLGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS720145. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PLGF. It may not necessarily be applicable to this product.
 NCBI GI #    6679289
 NCBI GeneID    18654
 NCBI Accession #    NP_032853.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008827.3 [Other Products]
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 UniProt Related Accession #    P49764 [Other Products]
 Molecular Weight    17,876 Da
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 NCBI Official Full Name    placenta growth factor isoform 1
 NCBI Official Synonym Full Names    placental growth factor
 NCBI Official Symbol    Pgf [Similar Products]
 NCBI Official Synonym Symbols   
PIGF; Plgf; AI854365
[Similar Products]
 NCBI Protein Information    placenta growth factor
 UniProt Protein Name    Placenta growth factor
 Protein Family    Placenta growth factor
 UniProt Gene Name    Pgf [Similar Products]
 UniProt Synonym Gene Names    Plgf; PlGF [Similar Products]
 UniProt Entry Name    PLGF_MOUSE
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 UniProt Comments for PLGF    PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; protein heterodimerization activity

Biological Process: positive regulation of angiogenesis; ureteric bud branching; multicellular organismal development; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; angiogenesis; sprouting angiogenesis; cell differentiation
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 Research Articles on PLGF    1. These results render PlGF an appealing target for ocular wound healing and point to potential therapeutic benefits of PlGF inhibition for the prevention of surgical failure.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PLGF elisa kitPathways associated with PLGF elisa kit
 Reference Product  PubMed Publications
 Vegfa elisa kit  >24 publications with PLGF and Vegfa
 Flt1 elisa kit  >18 publications with PLGF and Flt1
 Kdr elisa kit  >12 publications with PLGF and Kdr
 Vegfc elisa kit  >8 publications with PLGF and Vegfc
 Vegfb elisa kit  >5 publications with PLGF and Vegfb
 Nrp1 elisa kit  >3 publications with PLGF and Nrp1
 Figf elisa kit  >3 publications with PLGF and Figf
 Flt4 elisa kit  >3 publications with PLGF and Flt4
 Akt1 elisa kit  >2 publications with PLGF and Akt1
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 PI3K-Akt Signaling Pathway antibodies  PI3K-Akt Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Rap1 Signaling Pathway antibodies  Rap1 Signaling Pathway Diagram
 Ras Signaling Pathway antibodies  Ras Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
Diseases associated with PLGF elisa kitOrgans/Tissues associated with PLGF elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >380 publications with PLGF and Cardiovascular Diseases
 Inflammation Antibodies  >283 publications with PLGF and Inflammation
 Hypertension Antibodies  >194 publications with PLGF and Hypertension
 Disease Models, Animal Antibodies  >158 publications with PLGF and Disease Models, Animal
 Necrosis Antibodies  >153 publications with PLGF and Necrosis
 Lung Diseases Antibodies  >128 publications with PLGF and Lung Diseases
 Heart Diseases Antibodies  >127 publications with PLGF and Heart Diseases
 Atherosclerosis Antibodies  >92 publications with PLGF and Atherosclerosis
 Brain Diseases Antibodies  >72 publications with PLGF and Brain Diseases
 Kidney Diseases Antibodies  >69 publications with PLGF and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >238 publications with PLGF and Lung
 Embryonic Tissue Antibodies  >197 publications with PLGF and Embryonic Tissue
 Brain Antibodies  >195 publications with PLGF and Brain
 Liver Antibodies  >134 publications with PLGF and Liver
 Eye Antibodies  >83 publications with PLGF and Eye
 Stomach Antibodies  >70 publications with PLGF and Stomach
 Intestine Antibodies  >62 publications with PLGF and Intestine
 Pituitary Gland Antibodies  >32 publications with PLGF and Pituitary Gland
 Extraembryonic Tissue Antibodies  >31 publications with PLGF and Extraembryonic Tissue
 Spinal Cord Antibodies  >28 publications with PLGF and Spinal Cord
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