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PRA elisa kit :: Human Plasma Renin Activity ELISA Kit

Scan QR to view Datasheet Catalog #    MBS495044
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $455 +1 FREE 8GB USB
 
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 Product Name   

Plasma Renin Activity (PRA), ELISA Kit

★Popular Item★
 Also Known As   

Plasma Renin Activity (PRA) ELISA

 Product Gene Name   

PRA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Samples    Human plasma by an enzyme immunoassay.
 Assay Type    Competitive
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 Preparation and Storage    2 - 8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRA purchase    MBS495044 is a ready-to-use microwell, strip-or-full plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Plasma Renin Activity (PRA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRA. The ELISA analytical biochemical technique of the MBS495044 kit is based on PRA antibody-PRA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Human plasma by an enzyme immunoassay. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PRA elisa kit

   Intended Uses: For the determination of Plasma Renin Activity (PRA) in human plasma by an enzyme immunoassay. For research use only, not for use in diagnostic procedures.

Principle of the Assay: This kit measures PRA and the results are expressed in terms of mass of angiotensin-I (Ang-I) generated per volume of human plasma in unit time (ng/mL.h). The blood sample is collected in a tube that contains EDT A. The plasma is separated and either stored frozen or kept at room temperature for immediate use, samples should not be chilled on ice or stored at temperatures between 0 and 1Q degree C during collection or processing before adjustment of pH, this could lead to overestimation of renin activity. Before the start of immunoassay a protease inhibitor and the Generation buffer is added to the plasma sample, which will prevent Angiotensin-I (Ang-I) in plasma from degradation. The pH of the plasma sample should be around 6.0 after the addition of the supplied Generation buffer. The plasma sample is split in two and the fractions are incubated at 0-4 degree C (in ice bath) and 37 degree C respectively for 90 minutes or longer, to allow the generation of Ang-I by plasma renin at 3rC. Optionally, the pH can be adjusted to 6.5 or 7.4. Adjustment of pH is a critical step during the assay, acidification of plasma to pH 3.3 or lower for prolonged time with subsequent return to neutral pH causes irreversible activation of the renin (Derkx et aI., 1987), on the other side incubation at pH higher than 8.0 can destroy renin. During the immunoassay incubation, another set of protease inhibitors are involved, which function to stop the new generation as well as degradation of Ang-I to smaller peptides. The immunoassay of Ang-I is a competitive assay that uses two incubations, with a total assay incubation time of less than two hours. During the first incubation unlabelled Ang-I (present in the standards, controls and plasma samples) competes with biotinylated Ang-I to bind to the anti-Ang-I antibody. In the second incubation the labelled Streptavidin-HRP conjugate, binds to the immobilized Ang-I-Biotin. The washing and decanting procedures remove unbound materials. The colorimetric HRP substrate is added and after stopping the color development reaction, the light absorbance (00) is measured in a microwell plate reader. The absorbance values are inversely proportional to the concentration of Ang-I in the sample. A set of calibrators is used to plot a standard curve from which the concentrations of Ang-I in the samples and controls can be directly read.

Background: Measurement of PRA is important for the research of hypertensive subjects. In particular, determination of plasma renin activity can be useful in the study of primary hyperaldosteronism (5-13% of hypertensive cases) and assist in the therapy and management of other forms of hypertension. PRA, in contrast to the determination of renin concentration, is a more accurate indicator of primary hyperaldosteronism (PHA), because of several reasons: 1. PRA is the expression of the rate of Ang-I formation through the enzymatic action of renin on its substrate, angiotensinogen, therefore PRA depends not only on renin concentration but also on the concentration of angiotensinogen which is ignored in the renin concentration assay; 2. Plasma renin concentration assay does not ensure sensitivity in low renin states, while the sensitivity of the PRA assay can be enhanced by increasing the incubation time during the generation step (Sealey et aI., 2005), 3. When an inhibitor is bound to the renin active site PRA is inhibited, whereas the presence of the inhibitor does not affect the recognition of renin by currently available immunoassays, therefore total renin concentration does not always correlate with plasma renin activity (Campbell et aI., 2009). Renin liberates angiotensin-I from angiotensinogen. Angiotensin-I is transformed to angiotensin-II largely in pulmonary circulation by angiotensin converting enzyme (ACE). Angiotensin-II raises blood pressure by
 Product Categories/Family for PRA elisa kit    Cardiovascular; Research Assays; Assay Categories
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 Typical Testing Data/Standard Curve (for reference only) of PRA elisa kit    PRA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS495044. Click to request current manual
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 Protein Family    Proline-rich antigen
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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