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PODXL elisa kit :: Human Podocalyxin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765311
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Podocalyxin (PODXL), ELISA Kit

★Popular Item★
 Also Known As   

Human Podocalyxin ELISA Kit

 Product Synonym Names    PCX (Podocalyxin)/PODXL/PCLP/PCLP1/Podocalyxin-like protein 1/PC/PCLP-1/GCTM-2 antigen/Gp200
 Product Gene Name   

PODXL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765311 QC Report    QC Report PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of PODXL. No significant cross-reactivity or interference between PODXL and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    0.156-10ng/ml
 Sensitivity    0.094ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PODXL were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PODXL were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PODXL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PODXL purchase    MBS765311 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Podocalyxin (PODXL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/ml in biological research samples containing PODXL, with an estimated sensitivity of 0.094ng/ml. The ELISA analytical biochemical technique of the MBS765311 kit is based on PODXL antibody-PODXL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PODXL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PODXL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PODXL elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PODXL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PODXL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PODXL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PODXL can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of PODXL elisa kit    PODXL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS765311. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PODXL. It may not necessarily be applicable to this product.
 NCBI GI #    20301990
 NCBI GeneID    192181
 NCBI Accession #    NP_620203.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_138848.1 [Other Products]
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 UniProt Secondary Accession #    Q6IRK7; Q9R068 [Other Products]
 UniProt Related Accession #    Q9WTQ2 [Other Products]
 Molecular Weight    51,658 Da
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 NCBI Official Full Name    podocalyxin
 NCBI Official Synonym Full Names    podocalyxin-like
 NCBI Official Symbol    Podxl [Similar Products]
 NCBI Official Synonym Symbols   
PC; PCLP-1; podocalyxin
[Similar Products]
 NCBI Protein Information    podocalyxin
 UniProt Protein Name    Podocalyxin
 UniProt Synonym Protein Names   
Podocalyxin-like protein 1; PC; PCLP-1
 Protein Family    Podocalyxin
 UniProt Gene Name    Podxl [Similar Products]
 UniProt Synonym Gene Names    Pclp1; PC; PCLP-1 [Similar Products]
 UniProt Entry Name    PODXL_RAT
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 NCBI Summary for PODXL    inhibits cell-cell adhesion; may play a role in maintaining filtration in the glomerular epithelium [RGD, Feb 2006]
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 UniProt Comments for PODXL    PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: apical plasma membrane; cell projection; cytoplasm; extracellular space; filopodium; integral to membrane; intracellular membrane-bound organelle; lamellipodium; lipid raft; microvillus membrane; plasma membrane; ruffle

Molecular Function: protein binding

Biological Process: cell adhesion; cell migration; glomerular filtration; leukocyte migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of cell-cell adhesion; regulation of microvillus biogenesis
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 Research Articles on PODXL    1. Both the overexpression of CTGF and the loss of podocalyxin reflect renal damage in spontaneously hypertensive rats. CTGF and PCX may be involved in the mechanisms of podocyte injury and apoptosis induced by hypertension
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PODXL elisa kitDiseases associated with PODXL elisa kit
 Reference Product  PubMed Publications
 Wt1 elisa kit  >4 publications with PODXL and Wt1
 Nphs2 elisa kit  >3 publications with PODXL and Nphs2
 Spag9 elisa kit  >1 publications with PODXL and Spag9
 Ptpro elisa kit  >1 publications with PODXL and Ptpro
 Ezr elisa kit  >1 publications with PODXL and Ezr
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >12 publications with PODXL and Carcinoma
 Kidney Diseases Antibodies  >10 publications with PODXL and Kidney Diseases
 Liver Neoplasms Antibodies  >6 publications with PODXL and Liver Neoplasms
 Lung Neoplasms Antibodies  >5 publications with PODXL and Lung Neoplasms
 Liver Diseases Antibodies  >4 publications with PODXL and Liver Diseases
 Breast Neoplasms Antibodies  >4 publications with PODXL and Breast Neoplasms
 Nervous System Diseases Antibodies  >3 publications with PODXL and Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with PODXL and Brain Diseases
 Disease Models, Animal Antibodies  >3 publications with PODXL and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >3 publications with PODXL and Cardiovascular Diseases
Organs/Tissues associated with PODXL elisa kit
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >20 publications with PODXL and Kidney
 Embryonic Tissue Antibodies  >12 publications with PODXL and Embryonic Tissue
 Liver Antibodies  >7 publications with PODXL and Liver
 Muscle Antibodies  >6 publications with PODXL and Muscle
 Lung Antibodies  >6 publications with PODXL and Lung
 Brain Antibodies  >4 publications with PODXL and Brain
 Pancreas Antibodies  >3 publications with PODXL and Pancreas
 Prostate Antibodies  >2 publications with PODXL and Prostate
 Ovary Antibodies  >1 publications with PODXL and Ovary
 Eye Antibodies  >1 publications with PODXL and Eye
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