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PMF1 elisa kit :: Human Polyamine-modulated factor 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS281290 PMF1 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Polyamine-modulated factor 1 (PMF1), ELISA Kit

★Popular Item★
 Also Known As   

Human Polyamine-modulated factor 1 (PMF1) ELISA Kit

 Product Gene Name   

PMF1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    AF141309,  3D Structure    ModBase 3D Structure for Q6P1K2
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human PMF1. No significant cross-reactivity or interference between Human PMF1 and analogues was observed.
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 Samples    serum, plasma and other biological fluids
 Detection Range    0.31 ng/mL - 20 ng/mL
 Sensitivity    0.1 ng/mL (mean of 6 independent assays).
 Intra-assay Precision    Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PMF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PMF1 purchase    MBS281290 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Polyamine-modulated factor 1 (PMF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.31 ng/mL - 20 ng/mL in biological research samples containing PMF1, with an estimated sensitivity of 0.1 ng/mL (mean of 6 independent assays). The ELISA analytical biochemical technique of the MBS281290 kit is based on PMF1 antibody-PMF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PMF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PMF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PMF1 elisa kit

   Intended Uses: For the quantitative detection of Human Polyamine-modulated factor 1 (PMF1) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PMF1 in Human serum, plasma. An antibody specific for PMF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS281290. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PMF1. It may not necessarily be applicable to this product.
 NCBI GI #    313851053
 NCBI GeneID    11243
 NCBI Accession #    NP_001186582.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001199653.1 [Other Products]
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 UniProt Primary Accession #    Q6P1K2 [Other Products]
 UniProt Secondary Accession #    Q5TCJ8; Q5TCJ9; Q5TCK0; Q5TCK1; Q5TCK3; Q69YZ9; Q6PHR4; Q6ZVE6; Q86VJ6; Q8N4T6; A8K0C5 [Other Products]
 UniProt Related Accession #    Q6P1K2 [Other Products]
 Molecular Weight    20,230 Da
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 NCBI Official Full Name    polyamine-modulated factor 1 isoform 3
 NCBI Official Synonym Full Names    polyamine modulated factor 1
 NCBI Official Symbol    PMF1 [Similar Products]
 NCBI Protein Information    polyamine-modulated factor 1
 UniProt Protein Name    Polyamine-modulated factor 1
 Protein Family    Polyamine-modulated factor
 UniProt Gene Name    PMF1 [Similar Products]
 UniProt Synonym Gene Names    PMF-1 [Similar Products]
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 UniProt Comments for PMF1    Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.
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 Research Articles on PMF1    1. These results suggest that rs1052053 in PMF1 is not associated with leukoaraiosis (LA) risk in the Chinese population.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PMF1 elisa kitPathways associated with PMF1 elisa kit
 Reference Product  PubMed Publications
 NFE2L2 elisa kit  >6 publications with PMF1 and NFE2L2
 MIS12 elisa kit  >1 publications with PMF1 and MIS12
 COPS7A elisa kit  >1 publications with PMF1 and COPS7A
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic M-M/G1 Phases Pathway antibodies  Mitotic M-M/G1 Phases Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram
Organs/Tissues associated with PMF1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >1 publications with PMF1 and Placenta
 Bone Antibodies  >1 publications with PMF1 and Bone
 Heart Antibodies  >1 publications with PMF1 and Heart
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