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DHX58 elisa kit :: Human Probable ATP dependent RNA helicase DHX58 (DHX58) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7213202
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Probable ATP dependent RNA helicase DHX58 (DHX58), ELISA Kit

★Popular Item★
 Also Known As   

Human Probable ATP dependent RNA helicase DHX58 (DHX58) ELISA Kit

 Product Gene Name   

DHX58 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    608588
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of DHX58. No significant cross-reactivity or interference between DHX58 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DHX58 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DHX58 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DHX58 purchase    MBS7213202 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable ATP dependent RNA helicase DHX58 (DHX58) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHX58, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7213202 kit is based on DHX58 antibody-DHX58 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHX58 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHX58. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for DHX58 elisa kit

   Intended Uses: This DHX58 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DHX58. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: DHX58 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DHX58 antibody and an DHX58-HRP conjugate. The assay sample and buffer are incubated together with DHX58-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHX58 concentration since DHX58 from samples and DHX58-HRP conjugate compete for the anti-DHX58 antibody binding site. Since the number of sites is limited, as more sites are occupied by DHX58 from the sample, fewer sites are left to bind DHX58-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHX58 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for DHX58 elisa kit    Epigenetics and Nuclear Signaling
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 Typical Standard Curve/Testing Data of DHX58 elisa kit    DHX58 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS7213202. Click to request current manual
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NCBI/Uniprot data below describe general gene information for DHX58. It may not necessarily be applicable to this product.
 NCBI GI #    149408122
 NCBI GeneID    79132
 NCBI Accession #    NP_077024.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_024119.2 [Other Products]
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 UniProt Secondary Accession #    Q9HAM6 [Other Products]
 UniProt Related Accession #    Q96C10 [Other Products]
 Molecular Weight    76,613 Da [Similar Products]
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 NCBI Official Full Name    probable ATP-dependent RNA helicase DHX58
 NCBI Official Synonym Full Names    DEXH (Asp-Glu-X-His) box polypeptide 58
 NCBI Official Symbol    DHX58 [Similar Products]
 NCBI Official Synonym Symbols   
LGP2; RLR-3; D11LGP2; D11lgp2e
[Similar Products]
 NCBI Protein Information    probable ATP-dependent RNA helicase DHX58; RLR; RNA helicase LGP2; RIG-I-like receptor 3; protein D11Lgp2 homolog; RIG-I-like receptor LGP2; ortholog of mouse D11lgp2; probable ATP-dependent helicase LGP2
 UniProt Protein Name    Probable ATP-dependent RNA helicase DHX58
 UniProt Synonym Protein Names   
Probable ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RLR-3; RIG-I-like receptor LGP2; RLR
 Protein Family    Probable ATP-dependent RNA helicase
 UniProt Gene Name    DHX58 [Similar Products]
 UniProt Synonym Gene Names    D11LGP2E; LGP2; RLR-3; RLR [Similar Products]
 UniProt Entry Name    DHX58_HUMAN
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 UniProt Comments for DHX58    DHX58: Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1- dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. Belongs to the helicase family. RLR subfamily.

Protein type: Inhibitor; EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm

Molecular Function: protein binding; single-stranded RNA binding; DNA binding; zinc ion binding; double-stranded RNA binding; helicase activity; ATP binding

Biological Process: viral reproduction; metabolic process; negative regulation of innate immune response; response to virus; positive regulation of interferon type I production; innate immune response; defense response to virus; negative regulation of interferon type I production; regulation of innate immune response
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 Research Articles on DHX58    1. Experiments with paramyxovirus V protein-insensitive proteins revealed that the primary outcome of LGP2 interference is suppression of its ability to synergize with MDA5.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with DHX58 elisa kitPathways associated with DHX58 elisa kit
 Reference Product  PubMed Publications
 IFIH1 elisa kit  >30 publications with DHX58 and IFIH1
 DDX58 elisa kit  >24 publications with DHX58 and DDX58
 ISG15 elisa kit  >4 publications with DHX58 and ISG15
 RSAD2 elisa kit  >2 publications with DHX58 and RSAD2
 IRF7 elisa kit  >2 publications with DHX58 and IRF7
 EIF2AK2 elisa kit  >1 publications with DHX58 and EIF2AK2
 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
Diseases associated with DHX58 elisa kitOrgans/Tissues associated with DHX58 elisa kit
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >2 publications with DHX58 and Disease Models, Animal
 Respiratory Tract Diseases Antibodies  >2 publications with DHX58 and Respiratory Tract Diseases
 Inflammation Antibodies  >2 publications with DHX58 and Inflammation
 Kidney Diseases Antibodies  >1 publications with DHX58 and Kidney Diseases
 Nervous System Malformations Antibodies  >1 publications with DHX58 and Nervous System Malformations
 Necrosis Antibodies  >1 publications with DHX58 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with DHX58 and Blood
 Brain Antibodies  >4 publications with DHX58 and Brain
 Spleen Antibodies  >3 publications with DHX58 and Spleen
 Kidney Antibodies  >2 publications with DHX58 and Kidney
 Intestine Antibodies  >2 publications with DHX58 and Intestine
 Pancreas Antibodies  >1 publications with DHX58 and Pancreas
 Liver Antibodies  >1 publications with DHX58 and Liver
 Lung Antibodies  >1 publications with DHX58 and Lung
 Mouth Antibodies  >1 publications with DHX58 and Mouth
 Vascular Antibodies  >1 publications with DHX58 and Vascular
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