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GPR179 elisa kit :: Human Probable G-protein coupled receptor 179 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS280907 GPR179 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Probable G-protein coupled receptor 179 (GPR179), ELISA Kit

 Also Known As   

Human Probable G-protein coupled receptor 179 (GPR179) ELISA Kit

 Product Gene Name   

GPR179 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    AY584611 mRNA
 3D Structure    ModBase 3D Structure for Q6PRD1
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human GPR179. No significant cross-reactivity or interference between Human GPR179 and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GPR179 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GPR179 purchase    MBS280907 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable G-protein coupled receptor 179 (GPR179) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPR179. The ELISA analytical biochemical technique of the MBS280907 kit is based on GPR179 antibody-GPR179 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPR179 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPR179. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GPR179 elisa kit

   GPR179, Belongs to the G-protein coupled receptor 3 family. G protein-coupled receptors (GPCRs), comprise a large protein family of transmembrane receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses.
The class C G-protein-coupled receptors are a class of G-protein coupled receptors that include the metabotropic glutamate receptors and several additional receptors. Family C GPCRs have a large extracellular N-terminus which binds the orthosteric (endogenous) ligand. The shape of this domain is often likened to a clam. Several allosteric ligands to these receptors have been identified and these bind within the seven transmembrane region.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR179 in samples. An antibody specific for GPR179 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR179 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR179 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR179 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for GPR179. It may not necessarily be applicable to this product.
 NCBI GI #    629266054
 NCBI GeneID    440435
 NCBI Accession #    NP_001004334.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001004334.3 [Other Products]
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 UniProt Primary Accession #    Q6PRD1 [Other Products]
 UniProt Related Accession #    Q6PRD1 [Other Products]
 Molecular Weight    257,363 Da
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 NCBI Official Full Name    probable G-protein coupled receptor 179
 NCBI Official Synonym Full Names    G protein-coupled receptor 179
 NCBI Official Symbol    GPR179 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    probable G-protein coupled receptor 179
 UniProt Protein Name    Probable G-protein coupled receptor 179
 UniProt Synonym Protein Names   
Probable G-protein coupled receptor 158-like 1; GPR158-like
 Protein Family    Probable G-protein coupled receptor
 UniProt Gene Name    GPR179 [Similar Products]
 UniProt Synonym Gene Names    GPR158L; GPR158L1; GPR158-like [Similar Products]
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 NCBI Summary for GPR179    This gene encodes a member of the glutamate receptor subfamily of G protein-coupled receptors. The encoded protein has an EGF-like calcium binding domain and a seven transmembrane domain in the N-terminal region of the protein. Mutations in this gene are associated with congenital stationary night blindness type 1E. [provided by RefSeq, Apr 2012]
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 UniProt Comments for GPR179    GPR179: Orphan receptor, involved in vision. Required for signal transduction through retinal depolarizing bipolar cells. Defects in GPR179 are the cause of congenital stationary night blindness type 1E (CSNB1E). An autosomal recessive, non-progressive retinal disorder characterized by impaired night vision, absence of the electroretinogram (ERG) b- wave, and variable degrees of involvement of other visual functions. Affected individuals have an ERG waveform that lacks the b-wave because of failure to transmit the photoreceptor signal through the retinal depolarizing bipolar cells. Belongs to the G-protein coupled receptor 3 family.

Protein type: GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor signaling pathway; visual perception

Disease: Night Blindness, Congenital Stationary, Type 1e
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 Research Articles on GPR179    1. In this study, a novel compound heterozygous mutation, c.[1A>G]; [608G>T] (p.[0?]; p.[W203L]), was identified in the LRIT3 gene of a proband. No mutations were identified in the CABP4 or GPR179 gene.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GPR179 elisa kitDiseases associated with GPR179 elisa kit
 Reference Product  PubMed Publications
 TRPM1 elisa kit  >7 publications with GPR179 and TRPM1
 NYX elisa kit  >6 publications with GPR179 and NYX
 GRM6 elisa kit  >6 publications with GPR179 and GRM6
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >3 publications with GPR179 and Congenital Abnormalities
Organs/Tissues associated with GPR179 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >10 publications with GPR179 and Eye
 Brain Antibodies  >1 publications with GPR179 and Brain
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