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NAT8B elisa kit :: Human Probable N-acetyltransferase 8B ELISA Kit

Scan QR to view Datasheet Catalog #    MBS283341 NAT8B elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Probable N-acetyltransferase 8B (NAT8B), ELISA Kit

 Also Known As   

Human Probable N-acetyltransferase 8B (NAT8B) ELISA Kit

 Product Gene Name   

NAT8B elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Request for Current Manual Insert    Request Current Manual
 OMIM    AF185571 mRNA
 3D Structure    ModBase 3D Structure for Q9UHF3
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human NAT8B. No significant cross-reactivity or interference between Human NAT8B and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NAT8B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NAT8B purchase    MBS283341 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable N-acetyltransferase 8B (NAT8B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NAT8B. The ELISA analytical biochemical technique of the MBS283341 kit is based on NAT8B antibody-NAT8B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NAT8B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NAT8B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NAT8B elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NAT8B in samples. An antibody specific for NAT8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAT8B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAT8B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAT8B bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for NAT8B. It may not necessarily be applicable to this product.
 NCBI GI #    117168283
 NCBI GeneID    51471
 NCBI Accession #    NP_057431.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_016347.2 [Other Products]
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 UniProt Primary Accession #    Q9UHF3 [Other Products]
 UniProt Secondary Accession #    Q0VAD9; Q6NT18 [Other Products]
 UniProt Related Accession #    Q9UHF3 [Other Products]
 Molecular Weight    25,366 Da
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 NCBI Official Full Name    putative N-acetyltransferase 8B
 NCBI Official Synonym Full Names    N-acetyltransferase 8B (putative, gene/pseudogene)
 NCBI Official Symbol    NAT8B [Similar Products]
 NCBI Official Synonym Symbols   
CML2; Hcml2; NAT8BP
[Similar Products]
 NCBI Protein Information    putative N-acetyltransferase 8B
 UniProt Protein Name    Putative N-acetyltransferase 8B
 UniProt Synonym Protein Names   
Acetyltransferase 1; ATase1; Camello-like protein 2
 Protein Family    Putative N-acetyltransferase
 UniProt Gene Name    NAT8B [Similar Products]
 UniProt Synonym Gene Names    ATase1 [Similar Products]
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 NCBI Summary for NAT8B    The protein encoded by this gene is highly similar to the N-acetyltransferase 8 (NAT8) gene product, which is a kidney and liver protein with homology to bacterial acetyltransferases involved in drug resistance. This gene is localized on chromosome 2 in the vicinity of the NAT8 gene and may represent a pseudogene of NAT8. This gene contains two polymorphic nonsense mutations that disrupt the active site of the protein. The full-length product of this gene contains a complete acetyltransferase domain and is identical in length to NAT8. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NAT8B    NAT8B: May play a role in regulation of gastrulation. Belongs to the camello family.

Protein type: Acetyltransferase; EC 2.3.1.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum-Golgi intermediate compartment; endoplasmic reticulum-Golgi intermediate compartment membrane; integral component of membrane

Molecular Function: lysine N-acetyltransferase activity; N-acetyltransferase activity; protein binding

Biological Process: beta-amyloid metabolic process; cellular protein metabolic process; gastrulation with mouth forming second; negative regulation of apoptosis; peptidyl-lysine N6-acetylation
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 Research Articles on NAT8B    1. ATase1 and ATase2 are endoplasmic reticulum-based acetyltransferases that form homo- and heterodimers and associate with members of the oligosaccharyltransferase complex
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with NAT8B elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >1 publications with NAT8B and Kidney Diseases
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