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APIP elisa kit :: Human Probable methylthioribulose-1-phosphate dehydratase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS282228 APIP elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Probable methylthioribulose-1-phosphate dehydratase (APIP), ELISA Kit

★Popular Item★
 Also Known As   

Human Probable methylthioribulose-1-phosphate dehydratase (APIP) ELISA Kit

 Product Gene Name   

APIP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    AF132963 mRNA
 3D Structure    ModBase 3D Structure for Q96GX9
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human APIP. No significant cross-reactivity or interference between Human APIP and analogues was observed.
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 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
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 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of APIP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for APIP purchase    MBS282228 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable methylthioribulose-1-phosphate dehydratase (APIP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APIP. The ELISA analytical biochemical technique of the MBS282228 kit is based on APIP antibody-APIP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APIP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APIP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for APIP elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate APIP in samples. An antibody specific for APIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAPIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS282228. Click to request current manual
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NCBI/Uniprot data below describe general gene information for APIP. It may not necessarily be applicable to this product.
 NCBI GI #    166235186
 NCBI GeneID    51074
 NCBI Accession #    NP_057041.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_015957.3 [Other Products]
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 UniProt Primary Accession #    Q96GX9 [Other Products]
 UniProt Secondary Accession #    Q6PJX6; Q8WVU2; Q96HK2; Q9Y318; A8K9D3 [Other Products]
 UniProt Related Accession #    Q96GX9 [Other Products]
 Molecular Weight    22,858 Da
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 NCBI Official Full Name    methylthioribulose-1-phosphate dehydratase
 NCBI Official Synonym Full Names    APAF1 interacting protein
 NCBI Official Symbol    APIP [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    methylthioribulose-1-phosphate dehydratase
 UniProt Protein Name    Methylthioribulose-1-phosphate dehydratase
 UniProt Synonym Protein Names   
APAF1-interacting protein
 Protein Family    Probable methylthioribulose-1-phosphate dehydratase
 UniProt Gene Name    APIP [Similar Products]
 UniProt Synonym Gene Names    hAPIP [Similar Products]
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 NCBI Summary for APIP    APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008]
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 UniProt Comments for APIP    MMRP19: Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Has an anti-apoptotic function and prevents muscle ischemic damage. Inhibits the cytochrome c- dependent and APAF1-mediated cell death. Belongs to the aldolase class II family. MtnB subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; EC

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm

Molecular Function: identical protein binding; methylthioribulose 1-phosphate dehydratase activity; protein binding; zinc ion binding

Biological Process: apoptosis; L-methionine salvage from methylthioadenosine; negative regulation of apoptosis; protein homotetramerization; pyroptosis; regulation of ERK1 and ERK2 cascade
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 Research Articles on APIP    1. Results suggest that APIP stimulates cell proliferation via ERBB3, acting in conjunction with ERBB2, in gastric cancer cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with APIP elisa kitPathways associated with APIP elisa kit
 Reference Product  PubMed Publications
 CASP9 elisa kit  >7 publications with APIP and CASP9
 APAF1 elisa kit  >5 publications with APIP and APAF1
 Products by Pathway  Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Metabolism Of Polyamines Pathway antibodies  Metabolism Of Polyamines Pathway Diagram
 Methionine Salvage Pathway antibodies  Methionine Salvage Pathway Diagram
 Methionine Salvage Pathway antibodies  Methionine Salvage Pathway Diagram
 Methionine Salvage Pathway antibodies  Methionine Salvage Pathway Diagram
 S-methyl-5-thio-alpha-D-ribose 1-phosphate Degradation Pathway antibodies  S-methyl-5-thio-alpha-D-ribose 1-phosphate Degradation Pathway Diagram
Diseases associated with APIP elisa kitOrgans/Tissues associated with APIP elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with APIP and Inflammation
 Liver Diseases Antibodies  >1 publications with APIP and Liver Diseases
 Fibrosis Antibodies  >1 publications with APIP and Fibrosis
 Liver Neoplasms Antibodies  >1 publications with APIP and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with APIP and Lung
 Bone Antibodies  >1 publications with APIP and Bone
 Heart Antibodies  >1 publications with APIP and Heart
 Thymus Antibodies  >1 publications with APIP and Thymus
 Prostate Antibodies  >1 publications with APIP and Prostate
 Liver Antibodies  >1 publications with APIP and Liver
 Muscle Antibodies  >1 publications with APIP and Muscle
 Kidney Antibodies  >1 publications with APIP and Kidney
 Bone Marrow Antibodies  >1 publications with APIP and Bone Marrow
 Brain Antibodies  >1 publications with APIP and Brain
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