Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PCNA rightarrow Human PCNA  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • EDNRA - Endothelin A Receptor Antibody
 • Lysyl tRNA Synthetase (KARS) ELISA Kit
 • Cystatin-C (CST3) Recombinant Protein
 • Cyclic Citrullinated Peptide Antibody (CCP) ELISA Kit
 • Adenovirus (ADV) antibody (IgG) ELISA Kit
 • Arginase (Arg) ELISA Kit
 • CD300LG Antibody
 • FSH Antibody
 • beta-Defensins, beta-DF ELISA Kit
 • PSMC5 Antibody
 • Integrin beta3 (ITGB3) Antibody
 • PTP-1B (Protein Tyrosine Phosphatase1B) Recombinant Protein
 • D-3-phosphoglycerate dehydrogenase (PHGDH) Antibody
 • Acetate CoA-transferase subunit alpha (atoD) Recombinant Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PCNA elisa kit :: Human Proliferating Cell Nuclear Antigen ELISA Kit

Scan QR to view Datasheet Catalog #    MBS034032 PCNA elisa kit
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $680 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Proliferating Cell Nuclear Antigen (PCNA), ELISA Kit

★Popular Item★
 Also Known As   

Human Proliferating Cell Nuclear Antigen ELISA Kit

 Product Gene Name   

PCNA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 20; NC_000020.10 (5095599..5107268, complement). Location: 20pter-p12
 3D Structure    ModBase 3D Structure for Q6FHF5
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    No significant cross-reactivity or interference between Human PCNA and analogues was observed.
Section Bar
 Samples    Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
 Assay Type    Sandwich
 Detection Range    0.25 ng/ml - 8 ng/ml.
 Sensitivity    0.1 ng/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCNA concentrations in Human serum, plasma and other body fluids. Using Purified Human PCNA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCNA and PCNA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCNA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PCNA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PCNA purchase    MBS034032 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.25 ng/ml - 8 ng/ml in biological research samples containing PCNA, with an estimated sensitivity of 0.1 ng/ml. The ELISA analytical biochemical technique of the MBS034032 kit is based on PCNA antibody-PCNA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCNA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCNA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for PCNA elisa kit

   Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCNA concentrations in Human serum, plasma and other body fluids. Using Purified Human PCNA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCNA and PCNA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCNA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS034032. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PCNA. It may not necessarily be applicable to this product.
 NCBI GI #    49456555
 NCBI GeneID    5111
 NCBI Accession #    CAG46598.1 [Other Products]
Section Bar
 UniProt Primary Accession #    Q6FHF5 [Other Products]
 UniProt Related Accession #    P12004 [Other Products]
 Molecular Weight    28,706 Da
Table BarTOPTable Bar
 NCBI Official Full Name    PCNA
 NCBI Official Synonym Full Names    proliferating cell nuclear antigen
 NCBI Official Symbol    PCNA [Similar Products]
 NCBI Protein Information    proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
 UniProt Protein Name    Proliferating cell nuclear antigen
 Protein Family    Proliferating cell nuclear antigen
 UniProt Gene Name    PCNA [Similar Products]
 UniProt Entry Name    Q6FHF5_HUMAN
Section Bar
 NCBI Summary for PCNA    The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for PCNA    PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; DNA replication factor C complex; nuclear replication fork; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; purine-specific mismatch base pair DNA N-glycosylase activity; MutLalpha complex binding; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding

Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; response to lipid; DNA repair; leading strand elongation; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle

Disease: Ataxia-telangiectasia-like Disorder 2
Table BarTOPTable Bar
 Research Articles on PCNA    1. nucleosome binding protein HMGN1 as a new PCNA-interacting protein that enhances the binding of PCNA to chromatin
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with PCNA elisa kitOrgans/Tissues associated with PCNA elisa kit
 Products by Pathway  Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2407 publications with PCNA and Blood
 Liver Antibodies  >1847 publications with PCNA and Liver
 Vascular Antibodies  >1841 publications with PCNA and Vascular
 Muscle Antibodies  >1576 publications with PCNA and Muscle
 Brain Antibodies  >1017 publications with PCNA and Brain
 Kidney Antibodies  >966 publications with PCNA and Kidney
 Lung Antibodies  >888 publications with PCNA and Lung
 Skin Antibodies  >807 publications with PCNA and Skin
 Bone Antibodies  >744 publications with PCNA and Bone
 Lymph Antibodies  >635 publications with PCNA and Lymph
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions