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PRAP1 elisa kit :: Human Proline-rich acidic protein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765465
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
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 Product Name   

Proline-rich acidic protein 1 (PRAP1), ELISA Kit

★Popular Item★
 Also Known As   

Human Proline-rich acidic protein 1 ELISA Kit

 Product Synonym Names    PRAP1 (Proline-rich acidic protein 1)/Uterine-specific proline-rich acidic protein/Uterine-specific proline-rich acidic protein
 Product Gene Name   

PRAP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765465 COA    COA PDF
 OMIM    609776
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 Species Reactivity    Human
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    78-5000pg/ml
 Sensitivity    46.9pg/ml
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PRAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PRAP1 purchase    MBS765465 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proline-rich acidic protein 1 (PRAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000pg/ml in biological research samples containing PRAP1, with an estimated sensitivity of 46.9pg/ml. The ELISA analytical biochemical technique of the MBS765465 kit is based on PRAP1 antibody-PRAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for PRAP1 elisa kit

   Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PRAP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PRAP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PRAP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PRAP1 can be calculated.
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 Typical Testing Data of PRAP1 elisa kit    PRAP1 elisa kit Typical Testing Data image
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 Typical Standard Curve of PRAP1 elisa kit    PRAP1 elisa kit Typical Standard Curve image
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Sample Manual Insert of MBS765465. Click to request current manual
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NCBI/Uniprot data below describe general gene information for PRAP1. It may not necessarily be applicable to this product.
 NCBI GI #    223633959
 NCBI GeneID    118471
 NCBI Accession #    NP_660203.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_145202.4 [Other Products]
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 UniProt Secondary Accession #    Q5VWY4; Q7Z4X5; Q8IWR3; Q8NCS2; B7ZL57; B7ZL58; E9KL31 [Other Products]
 UniProt Related Accession #    Q96NZ9 [Other Products]
 Molecular Weight    16,332 Da
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 NCBI Official Full Name    proline-rich acidic protein 1 isoform 1
 NCBI Official Synonym Full Names    proline-rich acidic protein 1
 NCBI Official Symbol    PRAP1 [Similar Products]
 NCBI Official Synonym Symbols   
UPA; PRO1195
[Similar Products]
 NCBI Protein Information    proline-rich acidic protein 1
 UniProt Protein Name    Proline-rich acidic protein 1
 UniProt Synonym Protein Names   
Epididymis tissue protein Li 178; Uterine-specific proline-rich acidic protein
 Protein Family    Proline-rich acidic protein
 UniProt Gene Name    PRAP1 [Similar Products]
 UniProt Synonym Gene Names    UPA [Similar Products]
 UniProt Entry Name    PRAP1_HUMAN
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 UniProt Comments for PRAP1    PRAP1: May play an important role in maintaining normal growth homeostasis in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: extracellular region

Molecular Function: protein binding
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 Research Articles on PRAP1    1. PRAP1 is a protein interacting partner of MAD1 and that PRAP1 is able to down-regulate MAD1 and suppress mitotic checkpoint signalling in hepatocellular carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PRAP1 elisa kitDiseases associated with PRAP1 elisa kit
 Reference Product  PubMed Publications
 ZNF511 elisa kit  >1 publications with PRAP1 and ZNF511
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >1 publications with PRAP1 and Liver Diseases
 Adenocarcinoma Antibodies  >1 publications with PRAP1 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >1 publications with PRAP1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with PRAP1 and Liver Neoplasms
Organs/Tissues associated with PRAP1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Uterus Antibodies  >3 publications with PRAP1 and Uterus
 Liver Antibodies  >1 publications with PRAP1 and Liver
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