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CFP elisa kit :: Human Properdin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765961
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Properdin (CFP), ELISA Kit

 Also Known As   

Human Properdin ELISA Kit

 Product Synonym Names    CFP/Complement Factor P/Properdin/PFC/BFD/complement factor properdin/PFCcomplement
 Product Gene Name   

CFP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765961 COA    COA PDF
 OMIM    300383
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of CFP. No significant cross-reactivity or interference between CFP and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    1.563-100ng/ml
 Sensitivity    <0.938ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CFP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CFP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CFP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CFP purchase    MBS765961 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Properdin (CFP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.563-100ng/ml in biological research samples containing CFP, with an estimated sensitivity of <0.938ng/ml. The ELISA analytical biochemical technique of the MBS765961 kit is based on CFP antibody-CFP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CFP elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CFP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CFP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CFP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CFP can be calculated.
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 Typical Standard Curve/Testing Data of CFP elisa kit    CFP elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS765961. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CFP. It may not necessarily be applicable to this product.
 NCBI GI #    223671861
 NCBI GeneID    5199
 NCBI Accession #    NP_001138724.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145252.1 [Other Products]
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 UniProt Secondary Accession #    O15134; O15135; O15136; O75826 [Other Products]
 UniProt Related Accession #    P27918 [Other Products]
 Molecular Weight    51,276 Da
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 NCBI Official Full Name    properdin
 NCBI Official Synonym Full Names    complement factor properdin
 NCBI Official Symbol    CFP [Similar Products]
 NCBI Official Synonym Symbols   
BFD; PFC; PFD; PROPERDIN
[Similar Products]
 NCBI Protein Information    properdin
 UniProt Protein Name    Properdin
 UniProt Synonym Protein Names   
Complement factor P
 Protein Family    Properdin
 UniProt Gene Name    CFP [Similar Products]
 UniProt Synonym Gene Names    PFC [Similar Products]
 UniProt Entry Name    PROP_HUMAN
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 NCBI Summary for CFP    This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Feb 2009]
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 UniProt Comments for CFP    CFP: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Defects in CFP are the cause of properdin deficiency (PFD). PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space

Biological Process: cellular protein metabolic process; complement activation; complement activation, alternative pathway; defense response to bacterium; immune response; innate immune response; post-translational protein modification; protein amino acid O-linked glycosylation; regulation of complement activation

Disease: Properdin Deficiency, X-linked
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 Research Articles on CFP    1. can directly interact with neutrophil myeloperoxidase resulting in activation of alternative pathway of complement
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CFP elisa kitDiseases associated with CFP elisa kit
 Products by Pathway  Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-glycosylation Of TSR Domain-containing Proteins Pathway antibodies  O-glycosylation Of TSR Domain-containing Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >429 publications with CFP and Cardiovascular Diseases
 Lung Diseases Antibodies  >327 publications with CFP and Lung Diseases
 Poisoning Antibodies  >111 publications with CFP and Poisoning
 Breast Neoplasms Antibodies  >100 publications with CFP and Breast Neoplasms
 Necrosis Antibodies  >86 publications with CFP and Necrosis
 Inflammation Antibodies  >74 publications with CFP and Inflammation
 Weight Loss Antibodies  >56 publications with CFP and Weight Loss
 Disease Models, Animal Antibodies  >54 publications with CFP and Disease Models, Animal
 Prostatic Diseases Antibodies  >52 publications with CFP and Prostatic Diseases
 Fibrosis Antibodies  >45 publications with CFP and Fibrosis
Organs/Tissues associated with CFP elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >883 publications with CFP and Blood
 Brain Antibodies  >179 publications with CFP and Brain
 Lung Antibodies  >167 publications with CFP and Lung
 Bone Antibodies  >158 publications with CFP and Bone
 Muscle Antibodies  >137 publications with CFP and Muscle
 Vascular Antibodies  >101 publications with CFP and Vascular
 Liver Antibodies  >72 publications with CFP and Liver
 Spleen Antibodies  >32 publications with CFP and Spleen
 Bone Marrow Antibodies  >28 publications with CFP and Bone Marrow
 Connective Tissue Antibodies  >23 publications with CFP and Connective Tissue
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