Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow PCSK2 rightarrow Human PCSK2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Helicobacter pylori antibody (IgG) ELISA Kit
 • Matrix Metalloproteinase 20 (MMP20) ELISA Kit
 • TMEM173 cDNA Clone
 • Actin, pan (Act5C) Antibody
 • V5 tag Antibody
 • Catalase (H2O2) Protein
 • ES-Ab ELISA Kit
 • Receptor Interacting Serine Threonine Kinase 2 (RIPK2) Antibody
 • ACTH Antibody
 • Holdfast attachment protein A (hfaA) Recombinant Protein
 • EYA3 Antibody
 • Peroxiredoxin-1 (PRDX1) Recombinant Protein
 • Heat-labile enterotoxin B subunit, LTB ELISA Kit
 • Chemokine C-C-Motif Ligand 16 (CCL16) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

PCSK2 elisa kit :: Human Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2000087 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $665 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2), ELISA Kit

 Also Known As   

Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) ELISA Kit

 Product Synonym Names    PC2; NEC2; SPC2; Prohormone Convertase 2; Neuroendocrine Convertase 2; KEX2-Like Endoprotease 2; Proprotein convertase 2
 Product Gene Name   

PCSK2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    162151
 3D Structure    ModBase 3D Structure for P16519
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2).
No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) and analogues was observed.
Section Bar
 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.112ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
Section Bar
 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in an a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of PCSK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PCSK2 purchase    MBS2000087 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing PCSK2, with an estimated sensitivity of < 0.112ng/mL. The ELISA analytical biochemical technique of the MBS2000087 kit is based on PCSK2 antibody-PCSK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCSK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCSK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for PCSK2 elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for PCSK2 elisa kit    Enzyme & Kinase
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of PCSK2 elisa kit    PCSK2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2000087. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PCSK2. It may not necessarily be applicable to this product.
 NCBI GI #    13543298
 NCBI GeneID    5126
 NCBI Accession #    AAH05815.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P16519 [Other Products]
 UniProt Secondary Accession #    Q14927; Q5JYQ1; Q8IWA8; Q9NQG3; Q9NUG1; Q9UJC6; B1ANH9; B4DFQ3 [Other Products]
 UniProt Related Accession #    P16519; AAB32656 [Other Products]
 Molecular Weight    68,580 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Proprotein convertase subtilisin/kexin type 2
 NCBI Official Synonym Full Names    proprotein convertase subtilisin/kexin type 2
 NCBI Official Symbol    PCSK2 [Similar Products]
 NCBI Official Synonym Symbols   
PC2; NEC2; SPC2; NEC 2; NEC-2
[Similar Products]
 NCBI Protein Information    neuroendocrine convertase 2
 UniProt Protein Name    Neuroendocrine convertase 2
 UniProt Synonym Protein Names   
KEX2-like endoprotease 2; Prohormone convertase 2; Proprotein convertase 2; PC2
 Protein Family    Neuroendocrine convertase
 UniProt Gene Name    PCSK2 [Similar Products]
 UniProt Synonym Gene Names    NEC2; NEC 2; PC2 [Similar Products]
 UniProt Entry Name    NEC2_HUMAN
Section Bar
 NCBI Summary for PCSK2    This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The protein undergoes an initial autocatalytic processing event and interacts with a neuroendocrine secretory protein in the ER, exits the ER and sorts to secretory granules, where it is cleaved and catalytically activated during intracellular transport. The encoded protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2014]
Section Bar
 UniProt Comments for PCSK2    PCSK2: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Belongs to the peptidase S8 family. Furin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.94; Protease

Chromosomal Location of Human Ortholog: 20p11.2

Cellular Component: extracellular space; transport vesicle; membrane; dendrite; perikaryon

Molecular Function: protein binding; serine-type endopeptidase activity; protein complex binding

Biological Process: insulin processing; nervous system development; protein autoprocessing; cellular protein metabolic process; proteolysis
Table BarTOPTable Bar
 Research Articles on PCSK2    1. in the processing of hypothalamic neuropeptides in huntington disease may partially arise from decreased PC1/3 and PC2 expressions.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with PCSK2 elisa kitPathways associated with PCSK2 elisa kit
 Reference Product  PubMed Publications
 INS elisa kit  >3 publications with PCSK2 and INS
 SCG5 elisa kit  >2 publications with PCSK2 and SCG5
 IAPP elisa kit  >1 publications with PCSK2 and IAPP
 THBD elisa kit  >1 publications with PCSK2 and THBD
 Products by Pathway  Pathway Diagram
 Insulin Processing Pathway antibodies  Insulin Processing Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
Diseases associated with PCSK2 elisa kitOrgans/Tissues associated with PCSK2 elisa kit
 Disease Name  Pubmed Publications
 Thyroid Diseases Antibodies  >5 publications with PCSK2 and Thyroid Diseases
 Diabetes Mellitus, Type 2 Antibodies  >5 publications with PCSK2 and Diabetes Mellitus, Type 2
 Carcinoma Antibodies  >4 publications with PCSK2 and Carcinoma
 Inflammation Antibodies  >3 publications with PCSK2 and Inflammation
 Hyperplasia Antibodies  >3 publications with PCSK2 and Hyperplasia
 Kidney Diseases Antibodies  >2 publications with PCSK2 and Kidney Diseases
 Necrosis Antibodies  >2 publications with PCSK2 and Necrosis
 Fatty Liver Antibodies  >2 publications with PCSK2 and Fatty Liver
 Arteriosclerosis Antibodies  >2 publications with PCSK2 and Arteriosclerosis
 Lung Diseases Antibodies  >1 publications with PCSK2 and Lung Diseases
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >14 publications with PCSK2 and Pancreas
 Brain Antibodies  >7 publications with PCSK2 and Brain
 Thyroid Antibodies  >6 publications with PCSK2 and Thyroid
 Lung Antibodies  >2 publications with PCSK2 and Lung
 Eye Antibodies  >2 publications with PCSK2 and Eye
 Ovary Antibodies  >2 publications with PCSK2 and Ovary
 Bone Antibodies  >2 publications with PCSK2 and Bone
 Muscle Antibodies  >2 publications with PCSK2 and Muscle
 Intestine Antibodies  >2 publications with PCSK2 and Intestine
 Skin Antibodies  >1 publications with PCSK2 and Skin
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions