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PDIA6 elisa kit :: Human Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019993 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Protein Disulfide Isomerase A6 (PDIA6), ELISA Kit

 Also Known As   

Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit

 Product Synonym Names    PDI-A6; TXNDC7; P5; ERp5; Thioredoxin Domain Containing 7(Protein Disulfide Isomerase); Protein Disulfide Isomerase-Associated 6; Endoplasmic reticulum protein 5
 Product Gene Name   

PDIA6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    611099
 3D Structure    ModBase 3D Structure for Q15084
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A6 (PDIA6).
No significant cross-reactivity or interference between Protein Disulfide Isomerase A6 (PDIA6) and analogues was observed.
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 Samples    Tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Typically less than 0.049ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A6 (PDIA6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A6 (PDIA6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of PDIA6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for PDIA6 purchase    MBS2019993 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Disulfide Isomerase A6 (PDIA6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing PDIA6, with an estimated sensitivity of Typically less than 0.049ng/mL. The ELISA analytical biochemical technique of the MBS2019993 kit is based on PDIA6 antibody-PDIA6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDIA6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDIA6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for PDIA6 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A6 (PDIA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A6 (PDIA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A6 (PDIA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A6 (PDIA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of PDIA6 elisa kit    PDIA6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2019993
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NCBI/Uniprot data below describe general gene information for PDIA6. It may not necessarily be applicable to this product.
 NCBI GI #    5031973
 NCBI GeneID    10130
 NCBI Accession #    NP_005733.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005742.3 [Other Products]
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 UniProt Primary Accession #    Q15084 [Other Products]
 UniProt Secondary Accession #    Q53RC7; Q6ZSH5; Q99778; B3KY95; B5MCQ5; B7Z254; B7Z4M8; F8WA83 [Other Products]
 UniProt Related Accession #    Q15084 [Other Products]
 Molecular Weight    53,261 Da
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 NCBI Official Full Name    protein disulfide-isomerase A6 isoform d
 NCBI Official Synonym Full Names    protein disulfide isomerase family A, member 6
 NCBI Official Symbol    PDIA6 [Similar Products]
 NCBI Official Synonym Symbols   
P5; ERP5; TXNDC7
[Similar Products]
 NCBI Protein Information    protein disulfide-isomerase A6
 UniProt Protein Name    Protein disulfide-isomerase A6
 UniProt Synonym Protein Names   
Endoplasmic reticulum protein 5; ER protein 5; ERp5; Protein disulfide isomerase P5; Thioredoxin domain-containing protein 7
 Protein Family    Protein disulfide-isomerase
 UniProt Gene Name    PDIA6 [Similar Products]
 UniProt Synonym Gene Names    ERP5; P5; TXNDC7; ER protein 5; ERp5 [Similar Products]
 UniProt Entry Name    PDIA6_HUMAN
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 NCBI Summary for PDIA6    Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM, Mar 2008]
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 UniProt Comments for PDIA6    PDIA6: May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. Belongs to the protein disulfide isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; EC 5.3.4.1

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; melanosome; plasma membrane

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; protein folding; unfolded protein response; cell redox homeostasis; apoptotic cell clearance
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 Research Articles on PDIA6    1. CDDP-resistant non-small lung cancer cells undergo profound remodeling of their endoplasmic reticulum (ER) proteome (>80 proteins identified by proteomics) and exhibit a dramatic overexpression of two protein disulfide isomerases, PDIA4 and PDIA6.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with PDIA6 elisa kitPathways associated with PDIA6 elisa kit
 Reference Product  PubMed Publications
 PDIA4 elisa kit  >5 publications with PDIA6 and PDIA4
 HSPA5 elisa kit  >4 publications with PDIA6 and HSPA5
 HSP90B1 elisa kit  >2 publications with PDIA6 and HSP90B1
 Products by Pathway  Pathway Diagram
 IRE1alpha Activates Chaperones Pathway antibodies  IRE1alpha Activates Chaperones Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Unfolded Protein Response (UPR) Pathway antibodies  Unfolded Protein Response (UPR) Pathway Diagram
 XBP1(S) Activates Chaperone Genes Pathway antibodies  XBP1(S) Activates Chaperone Genes Pathway Diagram
Diseases associated with PDIA6 elisa kitOrgans/Tissues associated with PDIA6 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with PDIA6 and Nervous System Diseases
 Liver Diseases Antibodies  >2 publications with PDIA6 and Liver Diseases
 Kidney Diseases Antibodies  >2 publications with PDIA6 and Kidney Diseases
 Inflammation Antibodies  >2 publications with PDIA6 and Inflammation
 Brain Diseases Antibodies  >1 publications with PDIA6 and Brain Diseases
 Cardiovascular Diseases Antibodies  >1 publications with PDIA6 and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with PDIA6 and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >1 publications with PDIA6 and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with PDIA6 and Blood
 Lung Antibodies  >4 publications with PDIA6 and Lung
 Brain Antibodies  >4 publications with PDIA6 and Brain
 Liver Antibodies  >3 publications with PDIA6 and Liver
 Vascular Antibodies  >2 publications with PDIA6 and Vascular
 Kidney Antibodies  >2 publications with PDIA6 and Kidney
 Pancreas Antibodies  >2 publications with PDIA6 and Pancreas
 Muscle Antibodies  >2 publications with PDIA6 and Muscle
 Bone Marrow Antibodies  >2 publications with PDIA6 and Bone Marrow
 Heart Antibodies  >2 publications with PDIA6 and Heart
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