Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource rightarrow ELISA Kit rightarrow F2RL3 rightarrow Human F2RL3  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
Our Best Sellers moreseparator
 • NLR family, pyrin domain containing 2 (NLRP2) ELISA Kit
 • M-CSF Antibody
 • Inositol monophosphatase 1 (IMPA1) ELISA Kit
 • Histone-binding protein RBBP4-A (rbbp4-a) Recombinant Protein
 • Aldehyde Dehydrogenase 18 Family, Member A1 (ALDH18A1) ELISA Kit
 • MIPU1 (ZNF667) Antibody
 • JAG1 Antibody
 • DESMIN Antibody
 • LPR1 Antibody
 • irisin ELISA Kit
 • Mouse Anti-Rat Kappa (kappa chain specific) Secondary Antibody
 • calpastatin (CAST) ELISA Kit
 • FACTOR VIIIc (F8) Antibody
 • C-kit Antibody
 downarrow more ...
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

F2RL3 elisa kit :: Human Proteinase-activated receptor 4 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS280268 F2RL3 elisa kit
Unit / Price
48-Strip-Wells  /  $425 +1 FREE 8GB USB
96-Strip-Wells  /  $720 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,160 +3 FREE 8GB USB
10x96-Strip-Wells  /  $6,055 +7 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Proteinase-activated receptor 4 (F2RL3), ELISA Kit

★Popular Item★
 Also Known As   

Human Proteinase-activated receptor 4 (F2RL3) ELISA Kit

 Product Gene Name   

F2RL3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Request for Current Manual Insert    Request Current Manual
 OMIM    AF080214 mRNA
 3D Structure    ModBase 3D Structure for Q96RI0
Section Bar
 Species Reactivity    Human
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Human F2RL3. No significant cross-reactivity or interference between Human F2RL3 and analogues was observed.
Section Bar
 Samples    Serum, Plasma, Other biological fluids
 Assay Type    Sandwich
 Sample Volume    1-200 uL
 Precision    Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
 Detection Wavelength    450 nm
Section Bar
 Preparation and Storage    Store at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of F2RL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for F2RL3 purchase    MBS280268 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proteinase-activated receptor 4 (F2RL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F2RL3. The ELISA analytical biochemical technique of the MBS280268 kit is based on F2RL3 antibody-F2RL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F2RL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F2RL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar

Related Product Information for F2RL3 elisa kit

   Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate F2RL3 in samples. An antibody specific for F2RL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyF2RL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F2RL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F2RL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for F2RL3. It may not necessarily be applicable to this product.
 NCBI GI #    89353783
 NCBI GeneID    9002
 NCBI Accession #    NP_003941.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003950.3 [Other Products]
Section Bar
 UniProt Primary Accession #    Q96RI0 [Other Products]
 UniProt Secondary Accession #    O76067; Q6DK42 [Other Products]
 UniProt Related Accession #    Q96RI0 [Other Products]
 Molecular Weight    41,133 Da
Table BarTOPTable Bar
 NCBI Official Full Name    proteinase-activated receptor 4 preproprotein
 NCBI Official Synonym Full Names    F2R like thrombin or trypsin receptor 3
 NCBI Official Symbol    F2RL3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    proteinase-activated receptor 4
 UniProt Protein Name    Proteinase-activated receptor 4
 UniProt Synonym Protein Names   
Coagulation factor II receptor-like 3; Thrombin receptor-like 3
 Protein Family    Proteinase-activated receptor
 UniProt Gene Name    F2RL3 [Similar Products]
 UniProt Synonym Gene Names    PAR4; PAR-4 [Similar Products]
Section Bar
 NCBI Summary for F2RL3    This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016]
Section Bar
 UniProt Comments for F2RL3    PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: extracellular region; integral component of plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; thrombin receptor activity

Biological Process: blood coagulation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); phospholipase C-activating G-protein coupled receptor signaling pathway; platelet activation; platelet dense granule organization and biogenesis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of Rho protein signal transduction; response to wounding; signal transduction
Table BarTOPTable Bar
 Research Articles on F2RL3    1. PAR4 is required for platelet procoagulant function during thrombus formation in human blood
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with F2RL3 elisa kitPathways associated with F2RL3 elisa kit
 Reference Product  PubMed Publications
 F2RL2 elisa kit  >1 publications with F2RL3 and F2RL2
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
Organs/Tissues associated with F2RL3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with F2RL3 and Lung
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions