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SP-A elisa kit :: Human Pulmonary surfatcant-associated protein A, SP-A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733343
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Pulmonary surfatcant-associated protein A, SP-A, ELISA Kit

★Popular Item★
 Also Known As   

Human Pulmonary surfatcant-associated protein A, SP-A ELISA Kit

 Product Synonym Names    Human Pulmonary surfatcant-associated protein A (SP-A) ELISA Kit; Human Pulmonary surfatcant-associated protein A, SP-A ELISA Kit; Pulmonary surfatcant-associated protein A, SP-A; Pulmonary surfatcant-associated protein A, SP-A ELISA Kit (Human)
 Product Gene Name   

SP-A elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 14; NC_000080.5 (41945077..41949662). Location: 14 B; 14 14.0 cM
 3D Structure    ModBase 3D Structure for P35242
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of SPA. No significant cross-reactivity or interference between SPA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPA and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Sandwich
 Detection Range    5.0-100 ng/mL
 Sensitivity    1.0 ng/mL
 Intended Uses    This SPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of SP-A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for SP-A purchase    MBS733343 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pulmonary surfatcant-associated protein A, SP-A, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100 ng/mL in biological research samples containing SP-A, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS733343 kit is based on SP-A antibody-SP-A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SP-A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SP-A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for SP-A elisa kit

   Principle of the assay: SPA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SPA. Standards or samples are then added to the microtiter plate wells and SPA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SPA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SPA are added to each well to "sandwich" the SPA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SPA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPA concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for SP-A elisa kit    Human ELISA Kit
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 Typical Testing Data/Standard Curve (for reference only) of SP-A elisa kit    SP-A elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS733343. Click to request current manual
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NCBI/Uniprot data below describe general gene information for SP-A. It may not necessarily be applicable to this product.
 NCBI GI #    260453
 NCBI GeneID    20387
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 UniProt Primary Accession #    P35242 [Other Products]
 UniProt Related Accession #    P35242; Q6P8S2; Q9CQI1 [Other Products]
 Molecular Weight    26,157 Da
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 NCBI Official Full Name    SP-A
 NCBI Official Synonym Full Names    surfactant associated protein A1
 NCBI Official Symbol    Sftpa1 [Similar Products]
 NCBI Official Synonym Symbols   
SP-A; Sftp1; Sftp-1
[Similar Products]
 NCBI Protein Information    pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1
 UniProt Protein Name    Pulmonary surfactant-associated protein A
 UniProt Gene Name    Sftpa1 [Similar Products]
 UniProt Synonym Gene Names    Sftp-1; Sftp1; Sftpa [Similar Products]
 UniProt Entry Name    SFTPA_MOUSE
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 UniProt Comments for SP-A    SFTPA2: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Defects in SFTPA2 are a cause of pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Belongs to the SFTPA family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: multivesicular body; extracellular space; rough endoplasmic reticulum; cytoplasmic vesicle

Biological Process: positive regulation of phagocytosis
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 Research Articles on SP-A    1. Flagellum-deficient (DeltafliC) bacteria produces adequate amounts of exoproteases to degrade SP-A through quorum-sensing.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with SP-A elisa kitOrgans/Tissues associated with SP-A elisa kit
 Products by Pathway  Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >59 publications with SP-A and Lung
 Embryonic Tissue Antibodies  >5 publications with SP-A and Embryonic Tissue
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