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RORA elisa kit :: Human RAR-related orphan receptor A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS910208
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (Limit 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RAR-related orphan receptor A (RORA), ELISA Kit

 Also Known As   

Human Nuclear receptor ROR-alpha, RORA ELISA Kit

 Product Synonym Names    Human Nuclear receptor ROR-alpha (RORA) ELISA kit; DKFZp686M2414; MGC119326; MGC119329; NR1F1; ROR1; ROR2; ROR3; RZR-ALPHA; RZRA; RAR-related orphan receptor alpha; ROR-alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orpha; RAR-related orphan receptor A
 Product Gene Name   

RORA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS910208 Testing Data    Testing Data PDF
 OMIM    600825
 3D Structure    ModBase 3D Structure for P35398
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human RORA. No significant cross-reactivity or interference between Human RORA and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    18.75 pg/ml-1200 pg/ml
 Sensitivity    4.68 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Transcription/Transcription regulation
 Protein Biological Process 3    Transcription
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of RORA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RORA purchase    MBS910208 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RAR-related orphan receptor A (RORA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.75 pg/ml-1200 pg/ml in biological research samples containing RORA, with an estimated sensitivity of 4.68 pg/ml. The ELISA analytical biochemical technique of the MBS910208 kit is based on RORA antibody-RORA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RORA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RORA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for RORA elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RORA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RORA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RORA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RORA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of RORA elisa kit    RORA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS910208
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NCBI/Uniprot data below describe general gene information for RORA. It may not necessarily be applicable to this product.
 NCBI GI #    4506577
 NCBI GeneID    6095
 NCBI Accession #    NP_002934.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002943.3 [Other Products]
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 UniProt Primary Accession #    P35398 [Other Products]
 UniProt Secondary Accession #    P35397; P35399; P45445; Q495X4; Q96H83 [Other Products]
 UniProt Related Accession #    P35398 [Other Products]
 Molecular Weight    58,975 Da
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 NCBI Official Full Name    nuclear receptor ROR-alpha isoform c
 NCBI Official Synonym Full Names    RAR-related orphan receptor A
 NCBI Official Symbol    RORA [Similar Products]
 NCBI Official Synonym Symbols   
ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
[Similar Products]
 NCBI Protein Information    nuclear receptor ROR-alpha; nuclear receptor RZR-alpha; transcription factor RZR-alpha; retinoid-related orphan receptor alpha; nuclear receptor subfamily 1 group F member 1; thyroid hormone nuclear receptor alpha variant 4; retinoic acid receptor-related orphan receptor alpha
 UniProt Protein Name    Nuclear receptor ROR-alpha
 UniProt Synonym Protein Names   
Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha
 Protein Family    Nuclear receptor
 UniProt Gene Name    RORA [Similar Products]
 UniProt Synonym Gene Names    NR1F1; RZRA [Similar Products]
 UniProt Entry Name    RORA_HUMAN
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 NCBI Summary for RORA    The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]
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 UniProt Comments for RORA    RORA iso1: Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. Monomer. Interacts (via the DNA-binding domain) with HIF1; the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. By hypoxia and melatonin. Widely expressed in a number of tissues. Activated by CaMK4. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; beta-catenin binding; steroid hormone receptor activity; oxysterol binding; transcription factor activity; transcription factor binding

Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; granule cell precursor proliferation; intracellular receptor-mediated signaling pathway; regulation of macrophage activation; positive regulation of transcription, DNA-dependent; positive regulation of circadian rhythm; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; muscle cell differentiation; regulation of transcription, DNA-dependent; xenobiotic metabolic process; negative regulation of inflammatory response; regulation of steroid metabolic process; cerebellar Purkinje cell differentiation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; angiogenesis; circadian regulation of gene expression; nitric oxide biosynthetic process; cGMP metabolic process; regulation of smoothened signaling pathway
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 Research Articles on RORA    1. The retinoid-related orphan receptor RORalpha promotes keratinocyte differentiation via FOXN1.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RORA elisa kitPathways associated with RORA elisa kit
 Reference Product  PubMed Publications
 CLOCK elisa kit  >38 publications with RORA and CLOCK
 NR1D1 elisa kit  >17 publications with RORA and NR1D1
 ARNTL elisa kit  >14 publications with RORA and ARNTL
 NPAS2 elisa kit  >10 publications with RORA and NPAS2
 NR1D2 elisa kit  >3 publications with RORA and NR1D2
 MEF2C elisa kit  >1 publications with RORA and MEF2C
 NCOA2 elisa kit  >1 publications with RORA and NCOA2
 EP300 elisa kit  >1 publications with RORA and EP300
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
Diseases associated with RORA elisa kitOrgans/Tissues associated with RORA elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >23 publications with RORA and Inflammation
 Nervous System Diseases Antibodies  >14 publications with RORA and Nervous System Diseases
 Liver Diseases Antibodies  >12 publications with RORA and Liver Diseases
 Disease Models, Animal Antibodies  >9 publications with RORA and Disease Models, Animal
 Brain Diseases Antibodies  >9 publications with RORA and Brain Diseases
 Breast Neoplasms Antibodies  >8 publications with RORA and Breast Neoplasms
 Atrophy Antibodies  >7 publications with RORA and Atrophy
 Carcinoma, Hepatocellular Antibodies  >6 publications with RORA and Carcinoma, Hepatocellular
 Necrosis Antibodies  >6 publications with RORA and Necrosis
 Liver Neoplasms Antibodies  >5 publications with RORA and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >40 publications with RORA and Brain
 Liver Antibodies  >19 publications with RORA and Liver
 Muscle Antibodies  >13 publications with RORA and Muscle
 Bone Antibodies  >11 publications with RORA and Bone
 Embryonic Tissue Antibodies  >7 publications with RORA and Embryonic Tissue
 Kidney Antibodies  >3 publications with RORA and Kidney
 Connective Tissue Antibodies  >3 publications with RORA and Connective Tissue
 Lymph Node Antibodies  >2 publications with RORA and Lymph Node
 Ovary Antibodies  >2 publications with RORA and Ovary
 Stomach Antibodies  >2 publications with RORA and Stomach
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