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| Product Name
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RAR-related orphan receptor A (RORA), ELISA Kit ★Popular Item★
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| Also Known As
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Human Nuclear receptor ROR-alpha, RORA ELISA Kit
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| Product Synonym Names
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Human Nuclear receptor ROR-alpha (RORA) ELISA kit; DKFZp686M2414; MGC119326; MGC119329; NR1F1; ROR1; ROR2; ROR3; RZR-ALPHA; RZRA; RAR-related orphan receptor alpha; ROR-alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orpha; RAR-related orphan receptor A
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| MBS910208 Testing Data
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| OMIM
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600825
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| 3D Structure
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ModBase 3D Structure for P35398
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| Species Reactivity
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Human
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| Specificity
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This assay has high sensitivity and excellent specificity for detection of Human RORA. No significant cross-reactivity or interference between Human RORA and analogues was observed.
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| Samples
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Serum, plasma, tissue homogenates, Cell lysates
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| Assay Type
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Sandwich
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| Detection Range
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18.75 pg/ml-1200 pg/ml
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| Sensitivity
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4.68 pg/ml
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| Intra-assay Precision
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Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
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| Detection Wavelength
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450 nm
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| Sample Volume
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50-100ul
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| Protein Biological Process 1
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Transcription/Transcription regulation
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| Protein Biological Process 3
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Transcription
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| Preparation and Storage
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Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of RORA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for RORA purchase
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MBS910208 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RAR-related orphan receptor A (RORA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 18.75 pg/ml-1200 pg/ml in biological research samples containing RORA, with an estimated sensitivity of 4.68 pg/ml. The ELISA analytical biochemical technique of the MBS910208 kit is based on RORA antibody-RORA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RORA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RORA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RORA elisa kit
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Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RORA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RORA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RORA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RORA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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| Typical Testing Data/Standard Curve (for reference only) of RORA elisa kit
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| Sample Manual Insert of MBS910208. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for RORA. It may not necessarily be applicable to this product. |
| NCBI GI #
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4506577
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| NCBI GeneID
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6095
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| NCBI Accession #
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NP_002934.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_002943.3
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[Other Products]
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| UniProt Primary Accession #
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P35398
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[Other Products]
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| UniProt Secondary Accession #
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P35397; P35399; P45445; Q495X4; Q96H83
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[Other Products]
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| UniProt Related Accession #
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P35398
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[Other Products]
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| Molecular Weight
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58,975 Da
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| NCBI Official Full Name
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nuclear receptor ROR-alpha isoform c
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| NCBI Official Synonym Full Names
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RAR-related orphan receptor A
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| NCBI Official Symbol
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RORA
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[Similar Products]
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| NCBI Official Synonym Symbols
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ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
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[Similar Products]
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| NCBI Protein Information
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nuclear receptor ROR-alpha; nuclear receptor RZR-alpha; transcription factor RZR-alpha; retinoid-related orphan receptor alpha; nuclear receptor subfamily 1 group F member 1; thyroid hormone nuclear receptor alpha variant 4; retinoic acid receptor-related orphan receptor alpha
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| UniProt Protein Name
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Nuclear receptor ROR-alpha
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| UniProt Synonym Protein Names
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Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha
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| Protein Family
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Nuclear receptor
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| UniProt Gene Name
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RORA
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[Similar Products]
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| UniProt Synonym Gene Names
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NR1F1; RZRA
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[Similar Products]
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| UniProt Entry Name
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RORA_HUMAN
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| NCBI Summary for RORA
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The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]
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| UniProt Comments for RORA
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RORA iso1: Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. Monomer. Interacts (via the DNA-binding domain) with HIF1; the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. By hypoxia and melatonin. Widely expressed in a number of tissues. Activated by CaMK4. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 15q22.2
Cellular Component: nucleoplasm; nucleus
Molecular Function: ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; beta-catenin binding; steroid hormone receptor activity; oxysterol binding; transcription factor activity; transcription factor binding
Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; granule cell precursor proliferation; intracellular receptor-mediated signaling pathway; regulation of macrophage activation; positive regulation of transcription, DNA-dependent; positive regulation of circadian rhythm; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; muscle cell differentiation; regulation of transcription, DNA-dependent; xenobiotic metabolic process; negative regulation of inflammatory response; regulation of steroid metabolic process; cerebellar Purkinje cell differentiation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; angiogenesis; circadian regulation of gene expression; nitric oxide biosynthetic process; cGMP metabolic process; regulation of smoothened signaling pathway
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| Research Articles on RORA
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1. The retinoid-related orphan receptor RORalpha promotes keratinocyte differentiation via FOXN1.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with RORA elisa kit | Pathways associated with RORA elisa kit |
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| Diseases associated with RORA elisa kit | Organs/Tissues associated with RORA elisa kit |
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ELISA Kit 
