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REST elisa kit :: Human RE1-silencing transcription factor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS922211
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RE1-silencing transcription factor (REST), ELISA Kit

★Popular Item★
 Also Known As   

Human RE1-silencing transcription factor, REST ELISA Kit

 Product Synonym Names    Human RE1-silencing transcription factor (REST) ELISA kit; NRSF; XBR; neuron restrictive silencer factor; repressor binding to the X2 box; RE1-silencing transcription factor
 Product Gene Name   

REST elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS922211 Testing Data    Testing Data PDF
 OMIM    600571
 3D Structure    ModBase 3D Structure for Q13127
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of human REST. No significant cross-reactivity or interference between human REST and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates
 Assay Type    Quantitative Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    <7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of REST elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for REST purchase    MBS922211 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RE1-silencing transcription factor (REST) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing REST, with an estimated sensitivity of <7.81 pg/ml. The ELISA analytical biochemical technique of the MBS922211 kit is based on REST antibody-REST antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REST antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REST. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for REST elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for REST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REST bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of REST elisa kit    REST elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS922211. Click to request current manual
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NCBI/Uniprot data below describe general gene information for REST. It may not necessarily be applicable to this product.
 NCBI GI #    301897977
 NCBI GeneID    5978
 NCBI Accession #    NP_001180437.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001193508.1 [Other Products]
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 UniProt Primary Accession #    Q13127 [Other Products]
 UniProt Secondary Accession #    Q12956; Q12957; Q13134; Q59ER1; Q8IWI3; A2RUE0; B9EGJ0 [Other Products]
 UniProt Related Accession #    Q13127 [Other Products]
 Molecular Weight    121,872 Da
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 NCBI Official Full Name    RE1-silencing transcription factor
 NCBI Official Synonym Full Names    RE1-silencing transcription factor
 NCBI Official Symbol    REST [Similar Products]
 NCBI Official Synonym Symbols   
XBR; NRSF
[Similar Products]
 NCBI Protein Information    RE1-silencing transcription factor; repressor binding to the X2 box; neural-restrictive silencer factor; neuron restrictive silencer factor; RE1-silencing transcription factor variant E1a/E2/E4; RE1-silencing transcription factor variant E1a/E2/E3/E5; RE1-silencing transcription factor variant E1b/E2/E3/E5; RE1-silencing transcription factor variant E1c/E2/E3/E5; RE1-silencing transcription factor variant E1a/E2/E3/E4c; RE1-silencing transcription factor variant E1c/E2g/E3/E4; RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i; RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i; RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4; RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j
 UniProt Protein Name    RE1-silencing transcription factor
 UniProt Synonym Protein Names   
Neural-restrictive silencer factor; X2 box repressor
 Protein Family    RE1-silencing transcription factor
 UniProt Gene Name    REST [Similar Products]
 UniProt Synonym Gene Names    NRSF; XBR [Similar Products]
 UniProt Entry Name    REST_HUMAN
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 NCBI Summary for REST    This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]
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 UniProt Comments for REST    REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; transcriptional repressor complex; cytosol; nucleus

Molecular Function: protein binding; outward rectifier potassium channel activity; metal ion binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; negative regulation of neuron differentiation; negative regulation of calcium ion-dependent exocytosis; regulation of transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of aldosterone biosynthetic process; positive regulation of caspase activity; hemopoietic progenitor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of neurogenesis; negative regulation of insulin secretion
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 Research Articles on REST    1. the crucial regulatory function of NRSF in the heart and its importance for maintaining normal cardiac integrity. [review]
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with REST elisa kitPathways associated with REST elisa kit
 Reference Product  PubMed Publications
 RCOR1 elisa kit  >31 publications with REST and RCOR1
 HTT elisa kit  >10 publications with REST and HTT
 STMN2 elisa kit  >5 publications with REST and STMN2
 SIN3B elisa kit  >4 publications with REST and SIN3B
 EHMT2 elisa kit  >1 publications with REST and EHMT2
 Products by Pathway  Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
Diseases associated with REST elisa kitOrgans/Tissues associated with REST elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >11961 publications with REST and Nervous System Diseases
 Neoplasms Antibodies  >6797 publications with REST and Neoplasms
 Hypertension Antibodies  >5424 publications with REST and Hypertension
 Brain Diseases Antibodies  >4159 publications with REST and Brain Diseases
 Myocardial Infarction Antibodies  >4029 publications with REST and Myocardial Infarction
 Fatigue Antibodies  >2848 publications with REST and Fatigue
 Carcinoma Antibodies  >2329 publications with REST and Carcinoma
 Congenital Abnormalities Antibodies  >2142 publications with REST and Congenital Abnormalities
 Drug Toxicity Antibodies  >1701 publications with REST and Drug Toxicity
 Liver Diseases Antibodies  >1315 publications with REST and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >33477 publications with REST and Blood
 Heart Antibodies  >23940 publications with REST and Heart
 Muscle Antibodies  >15325 publications with REST and Muscle
 Vascular Antibodies  >11369 publications with REST and Vascular
 Brain Antibodies  >8858 publications with REST and Brain
 Bone Antibodies  >4856 publications with REST and Bone
 Lung Antibodies  >4804 publications with REST and Lung
 Nerve Antibodies  >4446 publications with REST and Nerve
 Skin Antibodies  >3410 publications with REST and Skin
 Liver Antibodies  >2727 publications with REST and Liver
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