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RBM20 elisa kit :: Human RNA Binding Motif Protein 20 (RBM20) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023243 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve
Unit / Price
48-Strip-Wells  /  $610 +1 FREE 8GB USB
96-Strip-Wells  /  $830 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,265 +4 FREE 8GB USB
10x96-Strip-Wells  /  $5,870 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RNA Binding Motif Protein 20 (RBM20), ELISA Kit

 Also Known As   

RNA Binding Motif Protein 20 (RBM20) ELISA Kit

 Product Gene Name   

RBM20 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS2023243 COA    COA PDF
 OMIM    613171
 3D Structure    ModBase 3D Structure for Q5T481
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RBM20. No significant cross-reactivity or interference between RBM20 and analogues was observed.
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 Samples    Human tissue homogenates, cell lysates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.312-20ng/mL. The standard curve concentrations used for the ELISA's were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL.
 Sensitivity    0.114ng/mL.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RBM20 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RBM20 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Other Notes    Small volumes of RBM20 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RBM20 purchase    MBS2023243 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RNA Binding Motif Protein 20 (RBM20) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL. The standard curve concentrations used for the ELISA's were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL in biological research samples containing RBM20, with an estimated sensitivity of 0.114ng/mL. The ELISA analytical biochemical technique of the MBS2023243 kit is based on RBM20 antibody-RBM20 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RBM20 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RBM20. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Human tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for RBM20 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RBM20 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to RBM20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RBM20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RBM20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RBM20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve of RBM20 elisa kit    RBM20 elisa kit Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS2023243
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NCBI/Uniprot data below describe general gene information for RBM20. It may not necessarily be applicable to this product.
 NCBI GI #    197276594
 NCBI GeneID    282996
 NCBI Accession #    NP_001127835.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001134363.1 [Other Products]
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 UniProt Primary Accession #    Q5T481 [Other Products]
 UniProt Secondary Accession #    Q5JVI1; A6NIP5; B5A868 [Other Products]
 UniProt Related Accession #    Q5T481 [Other Products]
 Molecular Weight    134,357 Da
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 NCBI Official Full Name    RNA-binding protein 20
 NCBI Official Synonym Full Names    RNA binding motif protein 20
 NCBI Official Symbol    RBM20 [Similar Products]
 NCBI Protein Information    RNA-binding protein 20
 UniProt Protein Name    RNA-binding protein 20
 UniProt Synonym Protein Names   
RNA-binding motif protein 20
 Protein Family    RNA-binding protein
 UniProt Gene Name    RBM20 [Similar Products]
 UniProt Entry Name    RBM20_HUMAN
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 NCBI Summary for RBM20    This gene encodes a protein that binds RNA and regulates splicing. Mutations in this gene have been associated with familial dilated cardiomyopathy. [provided by RefSeq, Apr 2014]
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 UniProt Comments for RBM20    RBM20: RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin). Defects in RBM20 are the cause of cardiomyopathy dilated type 1DD (CMD1DD). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: nucleus

Molecular Function: zinc ion binding; RNA binding; nucleotide binding

Biological Process: RNA splicing; heart development; mRNA processing; positive regulation of RNA splicing

Disease: Cardiomyopathy, Dilated, 1dd
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 Research Articles on RBM20    1. In failing hearts, reduced expression of RBM20 affected alternative splicing of several direct targets, indicating that differences in RBM20 expression may affect cardiac function.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RBM20 elisa kitDiseases associated with RBM20 elisa kit
 Reference Product  PubMed Publications
 LDB3 elisa kit  >1 publications with RBM20 and LDB3
 CSRP3 elisa kit  >1 publications with RBM20 and CSRP3
 TNNC1 elisa kit  >1 publications with RBM20 and TNNC1
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >12 publications with RBM20 and Cardiovascular Diseases
 Musculoskeletal Diseases Antibodies  >1 publications with RBM20 and Musculoskeletal Diseases
 Nervous System Diseases Antibodies  >1 publications with RBM20 and Nervous System Diseases
 Fibrosis Antibodies  >1 publications with RBM20 and Fibrosis
 Hypertrophy Antibodies  >1 publications with RBM20 and Hypertrophy
Organs/Tissues associated with RBM20 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >10 publications with RBM20 and Muscle
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