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RNASE3/ECP elisa kit :: Human RNASE3/ECP ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2500372
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RNASE3/ECP, ELISA Kit

★Popular Item★
 Also Known As   

Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) ELISA Kit

 Product Gene Name   

RNASE3/ECP elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2500372 COA    COA PDF
 OMIM    131398
 3D Structure    ModBase 3D Structure for P12724
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 Species Reactivity    Human
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 Specificity    This kit recognizes natural and recombinantHumanRNASE3/ECP. No significant cross-reactivity or interference between HumanRNASE3/ECP and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    39.063-2500pg/mL
 Sensitivity    23.438pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RNASE3/ECP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RNASE3/ECP purchase    MBS2500372 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RNASE3/ECP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 39.063-2500pg/mL in biological research samples containing RNASE3/ECP, with an estimated sensitivity of 23.438pg/mL. The ELISA analytical biochemical technique of the MBS2500372 kit is based on RNASE3/ECP antibody-RNASE3/ECP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RNASE3/ECP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RNASE3/ECP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RNASE3/ECP elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanRNASE3/ECPconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RNASE3/ECP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RNASE3/ECPand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RNASE3/ECP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of RNASE3/ECP.You can calculate the concentration of RNASE3/ECPin the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of RNASE3/ECP elisa kit    RNASE3/ECP elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2500372. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RNASE3/ECP. It may not necessarily be applicable to this product.
 NCBI GI #    64654615
 NCBI GeneID    6037
 NCBI Accession #    AAH96061.1 [Other Products]
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 UniProt Primary Accession #    P12724 [Other Products]
 UniProt Secondary Accession #    Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9 [Other Products]
 UniProt Related Accession #    P12724 [Other Products]
 Molecular Weight    18,385 Da
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 NCBI Official Full Name    Ribonuclease, RNase A family, 3 (eosinophil cationic protein)
 NCBI Official Synonym Full Names    ribonuclease, RNase A family, 3
 NCBI Official Symbol    RNASE3 [Similar Products]
 NCBI Official Synonym Symbols   
ECP; RNS3
[Similar Products]
 NCBI Protein Information    eosinophil cationic protein; RNase 3; cytotoxic ribonuclease; ribonuclease 3
 UniProt Protein Name    Eosinophil cationic protein
 UniProt Synonym Protein Names   
Ribonuclease 3; RNase 3
 UniProt Gene Name    RNASE3 [Similar Products]
 UniProt Synonym Gene Names    ECP; RNS3; ECP; RNase 3 [Similar Products]
 UniProt Entry Name    ECP_HUMAN
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 NCBI Summary for RNASE3/ECP    The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
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 UniProt Comments for RNASE3/ECP    RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.

Protein type: Ribonuclease; Secreted; Secreted, signal peptide; EC 3.1.27.-

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: nucleic acid binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response
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 Research Articles on RNASE3/ECP    1. eosinophil cationic protein levels can be used to diagnosis atopic dermatitis but cannot distinguish between intrinsic and extrinsic disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RNASE3/ECP elisa kitPathways associated with RNASE3/ECP elisa kit
 Reference Product  PubMed Publications
 RNASE2 elisa kit  >5 publications with RNASE3/ECP and RNASE2
 MPO elisa kit  >5 publications with RNASE3/ECP and MPO
 EPX elisa kit  >4 publications with RNASE3/ECP and EPX
 IL5 elisa kit  >1 publications with RNASE3/ECP and IL5
 Products by Pathway  Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
Diseases associated with RNASE3/ECP elisa kitOrgans/Tissues associated with RNASE3/ECP elisa kit
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >74 publications with RNASE3/ECP and Hypersensitivity
 Respiratory Tract Diseases Antibodies  >65 publications with RNASE3/ECP and Respiratory Tract Diseases
 Respiratory Hypersensitivity Antibodies  >58 publications with RNASE3/ECP and Respiratory Hypersensitivity
 Asthma Antibodies  >56 publications with RNASE3/ECP and Asthma
 Lung Diseases Antibodies  >49 publications with RNASE3/ECP and Lung Diseases
 Lung Diseases, Obstructive Antibodies  >47 publications with RNASE3/ECP and Lung Diseases, Obstructive
 Inflammation Antibodies  >43 publications with RNASE3/ECP and Inflammation
 Rhinitis Antibodies  >21 publications with RNASE3/ECP and Rhinitis
 Eosinophilia Antibodies  >18 publications with RNASE3/ECP and Eosinophilia
 Rhinitis, Allergic, Seasonal Antibodies  >13 publications with RNASE3/ECP and Rhinitis, Allergic, Seasonal
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >87 publications with RNASE3/ECP and Blood
 Eye Antibodies  >2 publications with RNASE3/ECP and Eye
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