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RFTN1 elisa kit :: Human Raftlin (RFTN1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7241519
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Raftlin (RFTN1), ELISA Kit

★Popular Item★
 Also Known As   

Human Raftlin (RFTN1) ELISA Kit

 Product Gene Name   

RFTN1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RFTN1. No significant cross-reactivity or interference between RFTN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RFTN1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RFTN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RFTN1 purchase    MBS7241519 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Raftlin (RFTN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RFTN1, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS7241519 kit is based on RFTN1 antibody-RFTN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RFTN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RFTN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RFTN1 elisa kit

   Intended Uses: This RFTN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RFTN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: RFTN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RFTN1 antibody and an RFTN1-HRP conjugate. The assay sample and buffer are incubated together with RFTN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RFTN1 concentration since RFTN1 from samples and RFTN1-HRP conjugate compete for the anti-RFTN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RFTN1 from the sample, fewer sites are left to bind RFTN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RFTN1 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of RFTN1 elisa kit    RFTN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7241519. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RFTN1. It may not necessarily be applicable to this product.
 NCBI GI #    41872577
 NCBI GeneID    23180
 NCBI Accession #    NP_055965.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_015150.1 [Other Products]
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 UniProt Secondary Accession #    Q0D2G0; Q496Y2; Q4QQI7; Q5JB48; Q7Z7P2 [Other Products]
 UniProt Related Accession #    Q14699 [Other Products]
 Molecular Weight    63,146 Da
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 NCBI Official Full Name    raftlin
 NCBI Official Synonym Full Names    raftlin, lipid raft linker 1
 NCBI Official Symbol    RFTN1 [Similar Products]
 NCBI Official Synonym Symbols   
MIG2; PIG9; PIB10; RAFTLIN
[Similar Products]
 NCBI Protein Information    raftlin; raft-linking protein; proliferation-inducing protein 9; proliferation-inducing protein 10; cell migration-inducing gene 2 protein
 UniProt Protein Name    Raftlin
 UniProt Synonym Protein Names   
Cell migration-inducing gene 2 protein; Raft-linking protein
 Protein Family    Raftlin
 UniProt Gene Name    RFTN1 [Similar Products]
 UniProt Synonym Gene Names    KIAA0084; MIG2 [Similar Products]
 UniProt Entry Name    RFTN1_HUMAN
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 UniProt Comments for RFTN1    RFTN1: May play a pivotal role in the formation and/or maintenance of lipid rafts. May regulate B-cell antigen receptor- mediated signaling. Belongs to the raftlin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 3p24.3

Cellular Component: protein complex; cytoplasm; plasma membrane; endosome; lipid raft

Molecular Function: double-stranded RNA binding

Biological Process: protein transport into lipid raft; B cell receptor signaling pathway; response to exogenous dsRNA; T cell antigen processing and presentation; interleukin-17 production; dsRNA transport; toll-like receptor 3 signaling pathway; lipid raft formation; T cell receptor signaling pathway; growth
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 Research Articles on RFTN1    1. Mitogen-inducible Gene-2 expression is reduced in breast cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with RFTN1 elisa kitOrgans/Tissues associated with RFTN1 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with RFTN1 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >3 publications with RFTN1 and Eye
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