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ROMO1 elisa kit :: Human Reactive oxygen species modulator 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS764414
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $280 +1 FREE 8GB USB
96-Strip-Wells  /  $375 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Reactive oxygen species modulator 1 (ROMO1), ELISA Kit

★Popular Item★
 Also Known As   

Human Reactive oxygen species modulator 1 ELISA Kit

 Product Synonym Names    ROMO1/Reactive oxygen species modulator 1/ROS modulator 1/Protein MGR2 homolog/Mitochondrial targeting GxxxG motif protein/MTGM/Protein MGR2 homolog/Glyrichin
 Product Gene Name   

ROMO1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS764414 COA    COA PDF
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ROMO1 . No significant cross-reactivity or interference between ROMO1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    31.2-2000pg/ml
 Sensitivity    <18.75pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ROMO1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ROMO1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ROMO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ROMO1 purchase    MBS764414 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Reactive oxygen species modulator 1 (ROMO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.2-2000pg/ml in biological research samples containing ROMO1, with an estimated sensitivity of <18.75pg/ml. The ELISA analytical biochemical technique of the MBS764414 kit is based on ROMO1 antibody-ROMO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROMO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROMO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ROMO1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ROMO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ROMO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ROMO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ROMO1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of ROMO1 elisa kit    ROMO1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS764414. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ROMO1. It may not necessarily be applicable to this product.
 NCBI GI #    18152785
 NCBI GeneID    140823
 NCBI Accession #    NP_542786.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_080748.2 [Other Products]
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 UniProt Secondary Accession #    Q3MHD5; Q5QP16; Q9CQ98; Q9H1N2; A7M872; E1P5R9; E9KL28 [Other Products]
 UniProt Related Accession #    P60602 [Other Products]
 Molecular Weight    5,900 Da
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 NCBI Official Full Name    reactive oxygen species modulator 1
 NCBI Official Synonym Full Names    reactive oxygen species modulator 1
 NCBI Official Symbol    ROMO1 [Similar Products]
 NCBI Official Synonym Symbols   
MTGM; MTGMP; C20orf52; bA353C18.2
[Similar Products]
 NCBI Protein Information    reactive oxygen species modulator 1
 UniProt Protein Name    Reactive oxygen species modulator 1
 UniProt Synonym Protein Names   
Epididymis tissue protein Li 175; Glyrichin; Mitochondrial targeting GxxxG motif protein; MTGM; Protein MGR2 homolog
 Protein Family    Reactive oxygen species modulator
 UniProt Gene Name    ROMO1 [Similar Products]
 UniProt Synonym Gene Names    C20orf52; ROS modulator 1; MTGM [Similar Products]
 UniProt Entry Name    ROMO1_HUMAN
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 NCBI Summary for ROMO1    The protein encoded by this gene is a mitochondrial membrane protein that is responsible for increasing the level of reactive oxygen species (ROS) in cells. The protein also has antimicrobial activity against a variety of bacteria by inducing bacterial membrane breakage. [provided by RefSeq, Nov 2014]
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 UniProt Comments for ROMO1    ROMO1: Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Belongs to the MGR2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Mitochondrial; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion

Biological Process: defense response to bacterium; positive regulation of cell proliferation; replicative cell aging
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 Research Articles on ROMO1    1. These results demonstrate that tumor cell invasion in response to oxidative stress is associated with Romo1 expression and the NF-kappaB signaling pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ROMO1 elisa kitDiseases associated with ROMO1 elisa kit
 Reference Product  PubMed Publications
 BCL2L1 elisa kit  >3 publications with ROMO1 and BCL2L1
 MYC elisa kit  >1 publications with ROMO1 and MYC
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >3 publications with ROMO1 and Necrosis
 Liver Neoplasms Antibodies  >2 publications with ROMO1 and Liver Neoplasms
 Inflammation Antibodies  >2 publications with ROMO1 and Inflammation
 Liver Diseases Antibodies  >2 publications with ROMO1 and Liver Diseases
 Heart Diseases Antibodies  >1 publications with ROMO1 and Heart Diseases
 Dyslipidemias Antibodies  >1 publications with ROMO1 and Dyslipidemias
 Fibrosis Antibodies  >1 publications with ROMO1 and Fibrosis
 Lung Injury Antibodies  >1 publications with ROMO1 and Lung Injury
 Atrophy Antibodies  >1 publications with ROMO1 and Atrophy
 Weight Loss Antibodies  >1 publications with ROMO1 and Weight Loss
Organs/Tissues associated with ROMO1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >7 publications with ROMO1 and Lung
 Liver Antibodies  >4 publications with ROMO1 and Liver
 Blood Antibodies  >3 publications with ROMO1 and Blood
 Brain Antibodies  >3 publications with ROMO1 and Brain
 Vascular Antibodies  >2 publications with ROMO1 and Vascular
 Intestine Antibodies  >1 publications with ROMO1 and Intestine
 Bone Antibodies  >1 publications with ROMO1 and Bone
 Bone Marrow Antibodies  >1 publications with ROMO1 and Bone Marrow
 Heart Antibodies  >1 publications with ROMO1 and Heart
 Kidney Antibodies  >1 publications with ROMO1 and Kidney
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