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REPIN1 elisa kit :: Human Replication Initiator 1 (REPIN1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020591 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $380 +1 FREE 8GB USB
48-Strip-Wells  /  $565 +1 FREE 8GB USB
96-Strip-Wells  /  $765 +1 FREE 8GB USB
5x96-Strip-Wells  /  $3,130 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,670 +7 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Replication Initiator 1 (REPIN1), ELISA Kit

 Also Known As   

Replication Initiator 1 (REPIN1) ELISA Kit

 Product Synonym Names    AP4; RIP60; ZNF464; Zfp464; Replication Initiation Region Protein(60kD); Zinc Finger Protein 464; 60 kDa origin-specific DNA-binding protein; ATT-binding protein
 Product Gene Name   

REPIN1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for Q9BWE0
 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Replication Initiator 1 (REPIN1).
No significant cross-reactivity or interference between Replication Initiator 1 (REPIN1) and analogues was observed.
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 Samples    Tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    0.313-20ng/mL
 Sensitivity    Typically less than 0.129ng/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Replication Initiator 1 (REPIN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Replication Initiator 1 (REPIN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of REPIN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for REPIN1 purchase    MBS2020591 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Replication Initiator 1 (REPIN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.313-20ng/mL in biological research samples containing REPIN1, with an estimated sensitivity of Typically less than 0.129ng/mL. The ELISA analytical biochemical technique of the MBS2020591 kit is based on REPIN1 antibody-REPIN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REPIN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REPIN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for REPIN1 elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Initiator 1 (REPIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Replication Initiator 1 (REPIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Replication Initiator 1 (REPIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Replication Initiator 1 (REPIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of REPIN1 elisa kit    REPIN1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020591. Click to request current manual
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NCBI/Uniprot data below describe general gene information for REPIN1. It may not necessarily be applicable to this product.
 NCBI GI #    51105891
 NCBI GeneID    29803
 NCBI Accession #    EAL24475.1 [Other Products]
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 UniProt Primary Accession #    Q9BWE0 [Other Products]
 UniProt Secondary Accession #    Q7LE03; Q9BUZ6; Q9NZH2; Q9UMP5; C9J3L7; D3DWZ1 [Other Products]
 UniProt Related Accession #    Q9BWE0 [Other Products]
 Molecular Weight    69,920 Da
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 NCBI Official Full Name    replication initiator 1
 NCBI Official Synonym Full Names    replication initiator 1
 NCBI Official Symbol    REPIN1 [Similar Products]
 NCBI Official Synonym Symbols   
AP4; RIP60; ZNF464; Zfp464
[Similar Products]
 NCBI Protein Information    replication initiator 1
 UniProt Protein Name    Replication initiator 1
 UniProt Synonym Protein Names   
60 kDa origin-specific DNA-binding protein; 60 kDa replication initiation region protein; ATT-binding protein; DHFR oribeta-binding protein RIP60; Zinc finger protein 464
 Protein Family    Replication initiator
 UniProt Gene Name    REPIN1 [Similar Products]
 UniProt Synonym Gene Names    RIP60; ZNF464 [Similar Products]
 UniProt Entry Name    REPI1_HUMAN
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 UniProt Comments for REPIN1    REPIN1: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending. Homodimers and homomultimers. Found in a complex with RIP60 and RIP100.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; nuclear membrane; lipid particle; nuclear origin of replication recognition complex; nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: DNA replication
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 Research Articles on REPIN1    1. AP4 is transcriptionally activated by TGFB1 to inhibit phosphorylation of SMAD2 as a negative regulator of an activated TGFB receptor signaling on cell growth inhibition in non-small cell lung cancer
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with REPIN1 elisa kitOrgans/Tissues associated with REPIN1 elisa kit
 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >2 publications with REPIN1 and Lung Neoplasms
 Fatty Liver Antibodies  >2 publications with REPIN1 and Fatty Liver
 Cell Transformation, Neoplastic Antibodies  >2 publications with REPIN1 and Cell Transformation, Neoplastic
 Neoplasm Metastasis Antibodies  >2 publications with REPIN1 and Neoplasm Metastasis
 Neurobehavioral Manifestations Antibodies  >1 publications with REPIN1 and Neurobehavioral Manifestations
 Nervous System Diseases Antibodies  >1 publications with REPIN1 and Nervous System Diseases
 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >5 publications with REPIN1 and Adipose Tissue
 Connective Tissue Antibodies  >5 publications with REPIN1 and Connective Tissue
 Liver Antibodies  >4 publications with REPIN1 and Liver
 Lung Antibodies  >2 publications with REPIN1 and Lung
 Epididymis Antibodies  >1 publications with REPIN1 and Epididymis
 Nerve Antibodies  >1 publications with REPIN1 and Nerve
 Blood Antibodies  >1 publications with REPIN1 and Blood
 Muscle Antibodies  >1 publications with REPIN1 and Muscle
 Embryonic Tissue Antibodies  >1 publications with REPIN1 and Embryonic Tissue
 Brain Antibodies  >1 publications with REPIN1 and Brain
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