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RGMA elisa kit :: Human Repulsive guidance molecule A (RGMA) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7208752
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Repulsive guidance molecule A (RGMA), ELISA Kit

★Popular Item★
 Also Known As   

Human Repulsive guidance molecule A (RGMA) ELISA Kit

 Product Gene Name   

RGMA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    607362
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RGMA. No significant cross-reactivity or interference between RGMA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RGMA and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RGMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RGMA purchase    MBS7208752 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Repulsive guidance molecule A (RGMA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RGMA, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS7208752 kit is based on RGMA antibody-RGMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RGMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RGMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RGMA elisa kit

   Intended Uses: This RGMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RGMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: RGMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RGMA antibody and an RGMA-HRP conjugate. The assay sample and buffer are incubated together with RGMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RGMA concentration since RGMA from samples and RGMA-HRP conjugate compete for the anti-RGMA antibody binding site. Since the number of sites is limited, as more sites are occupied by RGMA from the sample, fewer sites are left to bind RGMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RGMA concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for RGMA elisa kit    Neuroscience
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  Typical Testing Data/Standard Curve (for reference only) of RGMA elisa kit    RGMA elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7208752. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RGMA. It may not necessarily be applicable to this product.
 NCBI GI #    261878467
 NCBI GeneID    56963
 NCBI Accession #    NP_001159761.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001166289.1 [Other Products]
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 UniProt Secondary Accession #    Q0JV97; Q8NC80; Q9H0E6; Q9NPM3; B2RTW1; B7Z5S8; F5GXQ7; F5GZU6; G3V518 [Other Products]
 UniProt Related Accession #    Q96B86 [Other Products]
 Molecular Weight    50,035 Da
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 NCBI Official Full Name    repulsive guidance molecule A isoform 2
 NCBI Official Synonym Full Names    repulsive guidance molecule family member a
 NCBI Official Symbol    RGMA [Similar Products]
 NCBI Official Synonym Symbols   
RGM
[Similar Products]
 NCBI Protein Information    repulsive guidance molecule A; RGM domain family, member A
 UniProt Protein Name    Repulsive guidance molecule A
 UniProt Synonym Protein Names   
RGM domain family member A
 Protein Family    Repulsive guidance molecule
 UniProt Gene Name    RGMA [Similar Products]
 UniProt Synonym Gene Names    RGM [Similar Products]
 UniProt Entry Name    RGMA_HUMAN
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 NCBI Summary for RGMA    This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
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 UniProt Comments for RGMA    RGMA: Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. Belongs to the repulsive guidance molecule (RGM) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: endoplasmic reticulum; plasma membrane

Molecular Function: coreceptor activity

Biological Process: BMP signaling pathway; axon guidance
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 Research Articles on RGMA    1. RGMa expression and promoter methylation status are closely related to colorectal cancer genesis and progression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RGMA elisa kitPathways associated with RGMA elisa kit
 Reference Product  PubMed Publications
 RGMB elisa kit  >21 publications with RGMA and RGMB
 BMP2 elisa kit  >5 publications with RGMA and BMP2
 BMPR2 elisa kit  >4 publications with RGMA and BMPR2
 NEO1 elisa kit  >3 publications with RGMA and NEO1
 BMP4 elisa kit  >2 publications with RGMA and BMP4
 SMAD9 elisa kit  >1 publications with RGMA and SMAD9
 SMAD5 elisa kit  >1 publications with RGMA and SMAD5
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Netrin-1 Signaling Pathway antibodies  Netrin-1 Signaling Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
Diseases associated with RGMA elisa kitOrgans/Tissues associated with RGMA elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with RGMA and Inflammation
 Necrosis Antibodies  >2 publications with RGMA and Necrosis
 Growth Disorders Antibodies  >2 publications with RGMA and Growth Disorders
 Abnormalities, Multiple Antibodies  >1 publications with RGMA and Abnormalities, Multiple
 Liver Neoplasms, Experimental Antibodies  >1 publications with RGMA and Liver Neoplasms, Experimental
 Edema Antibodies  >1 publications with RGMA and Edema
 Carcinoma, Hepatocellular Antibodies  >1 publications with RGMA and Carcinoma, Hepatocellular
 Congenital Abnormalities Antibodies  >1 publications with RGMA and Congenital Abnormalities
 Fibrosis Antibodies  >1 publications with RGMA and Fibrosis
 Glioblastoma Antibodies  >1 publications with RGMA and Glioblastoma
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >41 publications with RGMA and Brain
 Embryonic Tissue Antibodies  >18 publications with RGMA and Embryonic Tissue
 Muscle Antibodies  >12 publications with RGMA and Muscle
 Eye Antibodies  >8 publications with RGMA and Eye
 Kidney Antibodies  >3 publications with RGMA and Kidney
 Intestine Antibodies  >2 publications with RGMA and Intestine
 Lung Antibodies  >2 publications with RGMA and Lung
 Connective Tissue Antibodies  >2 publications with RGMA and Connective Tissue
 Pancreas Antibodies  >1 publications with RGMA and Pancreas
 Ovary Antibodies  >1 publications with RGMA and Ovary
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