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RETN elisa kit :: Human Resistin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175924
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $485 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Resistin (RETN), ELISA Kit

★Popular Item★
 Also Known As   

Human Resistin PicoKine ELISA Kit

 Product Synonym Names    Resistin; resistin; Adipose tissue specific secretory factor; Adipose tissue-specific secretory factor; ADSF; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine rich secreted protein A12 alpha like 2; Cysteine rich secreted protein FIZZ3; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ 3; FIZZ3; Found in inflammatory zone 3; HXCP 1; HXCP1; MGC126603; MGC126609; PRO1199; Resistin; Resistin delta2; RETN; RETN 1; RETN_HUMAN; RETN1; UNQ407; XCP 1; XCP1
 Product Gene Name   

RETN elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605565
 3D Structure    ModBase 3D Structure for Q9HD89
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 Species Reactivity    Human
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 Specificity    Natural and recombinant Human Resistin
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 Samples    Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
 Assay Type    Quantitative Sandwich
 Detection Range    78pg/ml-5000pg/ml
 Sensitivity    <3pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RETN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RETN purchase    MBS175924 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Resistin (RETN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78pg/ml-5000pg/ml in biological research samples containing RETN, with an estimated sensitivity of <3pg/ml. The ELISA analytical biochemical technique of the MBS175924 kit is based on RETN antibody-RETN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RETN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RETN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RETN elisa kit

   Principle of the Assay: Human Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Resistin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: K19-P108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Resistin amount of sample captured in plate.

Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
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 Typical Testing Data/Standard Curve (for reference only) of RETN elisa kit    Human Resistin PicoKine ELISA Kit standard curve
RETN elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175924. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RETN. It may not necessarily be applicable to this product.
 NCBI GI #    18202962
 NCBI GeneID    56729
 NCBI Accession #    Q9HD89.1 [Other Products]
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 UniProt Primary Accession #    Q9HD89 [Other Products]
 UniProt Secondary Accession #    Q540D9; Q76B53; D6W649 [Other Products]
 UniProt Related Accession #    Q9HD89 [Other Products]
 Molecular Weight    8,686 Da
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 NCBI Official Full Name    Resistin
 NCBI Official Synonym Full Names    resistin
 NCBI Official Symbol    RETN [Similar Products]
 NCBI Official Synonym Symbols   
ADSF; RSTN; XCP1; FIZZ3; RETN1
[Similar Products]
 NCBI Protein Information    resistin; resistin delta2; found in inflammatory zone 3; cysteine-rich secreted protein FIZZ3; adipose tissue-specific secretory factor; cysteine-rich secreted protein A12-alpha-like 2; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1
 UniProt Protein Name    Resistin
 UniProt Synonym Protein Names   
Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3
 Protein Family    Resistin
 UniProt Gene Name    RETN [Similar Products]
 UniProt Synonym Gene Names    FIZZ3; HXCP1; RSTN; ADSF [Similar Products]
 UniProt Entry Name    RETN_HUMAN
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 NCBI Summary for RETN    This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]
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 UniProt Comments for RETN    resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: fat cell differentiation; response to mechanical stimulus; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; response to insulin stimulus; aging

Disease: Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on RETN    1. Resistin levels in seminal plasma are correlated with other inflammatory cytokines and sperm quality, suggesting role in regulating inflammation of male reproductive system.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RETN elisa kitPathways associated with RETN elisa kit
 Reference Product  PubMed Publications
 LEP elisa kit  >9 publications with RETN and LEP
 SOCS3 elisa kit  >6 publications with RETN and SOCS3
 GHRL elisa kit  >5 publications with RETN and GHRL
 CD36 elisa kit  >4 publications with RETN and CD36
 NPY elisa kit  >3 publications with RETN and NPY
 AKT1 elisa kit  >2 publications with RETN and AKT1
 INSR elisa kit  >2 publications with RETN and INSR
 INS elisa kit  >2 publications with RETN and INS
 IGF1 elisa kit  >1 publications with RETN and IGF1
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
Diseases associated with RETN elisa kitOrgans/Tissues associated with RETN elisa kit
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >330 publications with RETN and Insulin Resistance
 Inflammation Antibodies  >113 publications with RETN and Inflammation
 Cardiovascular Diseases Antibodies  >106 publications with RETN and Cardiovascular Diseases
 Diabetes Mellitus, Type 2 Antibodies  >101 publications with RETN and Diabetes Mellitus, Type 2
 Necrosis Antibodies  >99 publications with RETN and Necrosis
 Atherosclerosis Antibodies  >58 publications with RETN and Atherosclerosis
 Hypertension Antibodies  >35 publications with RETN and Hypertension
 Metabolic Syndrome X Antibodies  >32 publications with RETN and Metabolic Syndrome X
 Heart Diseases Antibodies  >30 publications with RETN and Heart Diseases
 Coronary Disease Antibodies  >29 publications with RETN and Coronary Disease
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >379 publications with RETN and Blood
 Intestine Antibodies  >10 publications with RETN and Intestine
 Placenta Antibodies  >8 publications with RETN and Placenta
 Bone Marrow Antibodies  >5 publications with RETN and Bone Marrow
 Eye Antibodies  >2 publications with RETN and Eye
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