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ALDH1A1 elisa kit :: Human Retinal dehydrogenase 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS764893
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Retinal dehydrogenase 1 (ALDH1A1), ELISA Kit

★Popular Item★
 Also Known As   

Human Retinal dehydrogenase 1 ELISA Kit

 Product Synonym Names    RALDH1 (Retinal dehydrogenase 1)/ALDH1A1/ALDH-E1/PUMB1/acetaldehyde dehydrogenase 1/Aldehyde dehydrogenase family 1 member A1/ALDH class 1/ALDH1/ALDH11/ALHDII/retinaldehyde dehydrogenase 1
 Product Gene Name   

ALDH1A1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS764893 COA    COA PDF
 OMIM    100640
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of ALDH1A1. No significant cross-reactivity or interference between ALDH1A1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Detection Range    1.56-100mIU/ml
 Sensitivity    <0.938mIU/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALDH1A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALDH1A1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ALDH1A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ALDH1A1 purchase    MBS764893 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.56-100mIU/ml in biological research samples containing ALDH1A1, with an estimated sensitivity of <0.938mIU/ml. The ELISA analytical biochemical technique of the MBS764893 kit is based on ALDH1A1 antibody-ALDH1A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALDH1A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALDH1A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ALDH1A1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ALDH1A1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ALDH1A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ALDH1A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ALDH1A1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of ALDH1A1 elisa kit    ALDH1A1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS764893. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ALDH1A1. It may not necessarily be applicable to this product.
 NCBI GI #    21361176
 NCBI GeneID    216
 NCBI Accession #    NP_000680.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000689.4 [Other Products]
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 UniProt Secondary Accession #    O00768; Q5SYR1 [Other Products]
 UniProt Related Accession #    P00352 [Other Products]
 Molecular Weight    54,862 Da
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 NCBI Official Full Name    retinal dehydrogenase 1
 NCBI Official Synonym Full Names    aldehyde dehydrogenase 1 family member A1
 NCBI Official Symbol    ALDH1A1 [Similar Products]
 NCBI Official Synonym Symbols   
ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
[Similar Products]
 NCBI Protein Information    retinal dehydrogenase 1
 UniProt Protein Name    Retinal dehydrogenase 1
 UniProt Synonym Protein Names   
ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic
 Protein Family    Retinal dehydrogenase
 UniProt Gene Name    ALDH1A1 [Similar Products]
 UniProt Synonym Gene Names    ALDC; ALDH1; PUMB1; RALDH 1; RalDH1 [Similar Products]
 UniProt Entry Name    AL1A1_HUMAN
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 NCBI Summary for ALDH1A1    The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
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 UniProt Comments for ALDH1A1    ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.

Protein type: Oxidoreductase; GAPs; Cofactor and Vitamin Metabolism - retinol; EC 1.2.1.36; GAPs, Ras; Apoptosis

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; benzaldehyde dehydrogenase (NAD+) activity; GTPase activator activity; retinal dehydrogenase activity

Biological Process: aldehyde metabolic process; ethanol oxidation; positive regulation of GTPase activity; retinol metabolic process; xenobiotic metabolic process
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 Research Articles on ALDH1A1    1. High ALDH1 expression is associated with high-grade neuroendocrine carcinoma of the lung.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ALDH1A1 elisa kitPathways associated with ALDH1A1 elisa kit
 Reference Product  PubMed Publications
 ADH1B elisa kit  >12 publications with ALDH1A1 and ADH1B
 CYP26B1 elisa kit  >11 publications with ALDH1A1 and CYP26B1
 CYP26A1 elisa kit  >8 publications with ALDH1A1 and CYP26A1
 ADH4 elisa kit  >8 publications with ALDH1A1 and ADH4
 ADH1A elisa kit  >5 publications with ALDH1A1 and ADH1A
 ADH5 elisa kit  >4 publications with ALDH1A1 and ADH5
 ADH7 elisa kit  >3 publications with ALDH1A1 and ADH7
 CYP26C1 elisa kit  >2 publications with ALDH1A1 and CYP26C1
 ADH6 elisa kit  >2 publications with ALDH1A1 and ADH6
 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Ethanol Oxidation Pathway antibodies  Ethanol Oxidation Pathway Diagram
 Fatty Acid Omega Oxidation Pathway antibodies  Fatty Acid Omega Oxidation Pathway Diagram
 Folate-Alcohol And Cancer Pathway antibodies  Folate-Alcohol And Cancer Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Phase 1 - Functionalization Of Compounds Pathway antibodies  Phase 1 - Functionalization Of Compounds Pathway Diagram
 RA Biosynthesis Pathway antibodies  RA Biosynthesis Pathway Diagram
 Retinol Metabolism Pathway antibodies  Retinol Metabolism Pathway Diagram
 Retinol Metabolism Pathway antibodies  Retinol Metabolism Pathway Diagram
Diseases associated with ALDH1A1 elisa kitOrgans/Tissues associated with ALDH1A1 elisa kit
 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >34 publications with ALDH1A1 and Lung Neoplasms
 Breast Neoplasms Antibodies  >30 publications with ALDH1A1 and Breast Neoplasms
 Disease Models, Animal Antibodies  >25 publications with ALDH1A1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >24 publications with ALDH1A1 and Nervous System Diseases
 Brain Diseases Antibodies  >23 publications with ALDH1A1 and Brain Diseases
 Neoplasms, Experimental Antibodies  >20 publications with ALDH1A1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >18 publications with ALDH1A1 and Liver Diseases
 Parkinsonian Disorders Antibodies  >14 publications with ALDH1A1 and Parkinsonian Disorders
 Liver Neoplasms Antibodies  >13 publications with ALDH1A1 and Liver Neoplasms
 Inflammation Antibodies  >11 publications with ALDH1A1 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >56 publications with ALDH1A1 and Lung
 Liver Antibodies  >50 publications with ALDH1A1 and Liver
 Blood Antibodies  >49 publications with ALDH1A1 and Blood
 Brain Antibodies  >43 publications with ALDH1A1 and Brain
 Eye Antibodies  >32 publications with ALDH1A1 and Eye
 Prostate Antibodies  >17 publications with ALDH1A1 and Prostate
 Testis Antibodies  >15 publications with ALDH1A1 and Testis
 Bone Antibodies  >15 publications with ALDH1A1 and Bone
 Muscle Antibodies  >15 publications with ALDH1A1 and Muscle
 Intestine Antibodies  >14 publications with ALDH1A1 and Intestine
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