View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Human Rheumatoid Factor IgA ELISA Kit Catalog # MBS494689-ORG 522A Unit / Price 96 Tests / $415 +1 FREE 8GB USB 5x96 Tests / $1,900 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Rheumatoid Factor IgA, ELISA Kit Full Product Name Rheumatoid Factor IgA ELISA Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Request for Current Manual Insert Request Current Manual Species Reactivity Human Specificity The microplate is coated with the Fc fragment of highly purified human Immunoglobulin G. The test kit is specific for all classes of rheumatoid factors. Samples Human serum or plasma Sensitivity The lower detection limit for Rheumatoid Factor IgA was determined at 1.0 U/ml. Intended Uses Rheumatoid Factor IgA is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgA class rheumatoid factor antibodies in human serum or plasma. Preparation and Storage 2 - 8 degree C Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Rheumatoid Factor IgA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for Rheumatoid Factor IgA purchase MBS494689-ORG 522A is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rheumatoid Factor IgA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Rheumatoid Factor IgA. The ELISA analytical biochemical technique of the MBS494689-ORG 522A kit is based on Rheumatoid Factor IgA antibody-Rheumatoid Factor IgA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Rheumatoid Factor IgA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Rheumatoid Factor IgA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for Rheumatoid Factor IgA elisa kit Principle of the assay: Fc fragments of highly purified human Immunoglobulin G are bound to microwells. Antibodies against this antigen, if present in diluted serum or plasma, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgA immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyses to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgA antibodies present in the original sample. Product Categories/Family for Rheumatoid Factor IgA elisa kit Autoimmune; Immunology; Assay Categories Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP