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ROCK2 elisa kit :: Human Rho-associated, coiled-coil containing protein kinase 2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS705283
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $550 +1 FREE 8GB USB
96-Strip-Wells  /  $795 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), ELISA Kit

 Also Known As   

Human Rho-associated coiled-coil containing protein kinase 2, Rock-2 ELISA Kit

 Product Synonym Names    Human Rho-associated coiled-coil containing protein kinase 2 (Rock-2) ELISA Kit; KIAA0619; ; Rho-associated; coiled-coil containing protein kinase 2
 Product Gene Name   

ROCK2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS705283 Testing Data    Testing Data PDF
 OMIM    604002
 3D Structure    ModBase 3D Structure for O75116
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Human ROCK2. No significant cross-reactivity or interference between Human ROCK2 and analogues was observed.
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 Assay Type    Sandwich
 Detection Range    0.625 ng/ml-40 ng/ml
 Sensitivity    0.156ng/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of ROCK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for ROCK2 purchase    MBS705283 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rho-associated, coiled-coil containing protein kinase 2 (ROCK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.625 ng/ml-40 ng/ml in biological research samples containing ROCK2, with an estimated sensitivity of 0.156ng/ml. The ELISA analytical biochemical technique of the MBS705283 kit is based on ROCK2 antibody-ROCK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROCK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROCK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for ROCK2 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Rock-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Rock-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Rock-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Rock-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of ROCK2 elisa kit    ROCK2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS705283. Click to request current manual
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NCBI/Uniprot data below describe general gene information for ROCK2. It may not necessarily be applicable to this product.
 NCBI GI #    41872583
 NCBI GeneID    9475
 NCBI Accession #    NP_004841.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004850.3 [Other Products]
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 UniProt Primary Accession #    O75116 [Other Products]
 UniProt Secondary Accession #    Q53QZ0; Q53SJ7; Q9UQN5 [Other Products]
 UniProt Related Accession #    O75116 [Other Products]
 Molecular Weight    160,900 Da
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 NCBI Official Full Name    rho-associated protein kinase 2
 NCBI Official Synonym Full Names    Rho-associated, coiled-coil containing protein kinase 2
 NCBI Official Symbol    ROCK2 [Similar Products]
 NCBI Official Synonym Symbols   
ROCK-II
[Similar Products]
 NCBI Protein Information    rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II
 UniProt Protein Name    Rho-associated protein kinase 2
 UniProt Synonym Protein Names   
Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2
 Protein Family    Rho-associated protein kinase
 UniProt Gene Name    ROCK2 [Similar Products]
 UniProt Synonym Gene Names    KIAA0619; ROCK-II [Similar Products]
 UniProt Entry Name    ROCK2_HUMAN
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 NCBI Summary for ROCK2    The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ROCK2    Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca2+ sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.8 PublicationsManual assertion based on experiment in:Ref.4

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

Enzyme regulation: Activated by RHOA binding. Inhibited by Y-27632
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 Research Articles on ROCK2    1. Loss of TSC2 expression can alter microtubule organization and dynamics, which, in turn, deregulate cell division and potentially impair neuronal differentiation via ROCK2 signaling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with ROCK2 elisa kitPathways associated with ROCK2 elisa kit
 Reference Product  PubMed Publications
 RHOA elisa kit  >111 publications with ROCK2 and RHOA
 LIMK1 elisa kit  >12 publications with ROCK2 and LIMK1
 PPP1R12A elisa kit  >10 publications with ROCK2 and PPP1R12A
 RAC1 elisa kit  >9 publications with ROCK2 and RAC1
 CDC42 elisa kit  >4 publications with ROCK2 and CDC42
 PTEN elisa kit  >3 publications with ROCK2 and PTEN
 RND1 elisa kit  >1 publications with ROCK2 and RND1
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 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHA-mediated Growth Cone Collapse Pathway antibodies  EPHA-mediated Growth Cone Collapse Pathway Diagram
 EPHB-mediated Forward Signaling Pathway antibodies  EPHB-mediated Forward Signaling Pathway Diagram
Diseases associated with ROCK2 elisa kitOrgans/Tissues associated with ROCK2 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >65 publications with ROCK2 and Cardiovascular Diseases
 Hypertension Antibodies  >42 publications with ROCK2 and Hypertension
 Disease Models, Animal Antibodies  >38 publications with ROCK2 and Disease Models, Animal
 Inflammation Antibodies  >27 publications with ROCK2 and Inflammation
 Nervous System Diseases Antibodies  >25 publications with ROCK2 and Nervous System Diseases
 Brain Diseases Antibodies  >17 publications with ROCK2 and Brain Diseases
 Fibrosis Antibodies  >15 publications with ROCK2 and Fibrosis
 Neoplasm Metastasis Antibodies  >13 publications with ROCK2 and Neoplasm Metastasis
 Atherosclerosis Antibodies  >11 publications with ROCK2 and Atherosclerosis
 Breast Neoplasms Antibodies  >11 publications with ROCK2 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with ROCK2 and Bone
 Connective Tissue Antibodies  >4 publications with ROCK2 and Connective Tissue
 Heart Antibodies  >3 publications with ROCK2 and Heart
 Muscle Antibodies  >3 publications with ROCK2 and Muscle
 Brain Antibodies  >3 publications with ROCK2 and Brain
 Vascular Antibodies  >2 publications with ROCK2 and Vascular
 Embryonic Tissue Antibodies  >2 publications with ROCK2 and Embryonic Tissue
 Ear Antibodies  >1 publications with ROCK2 and Ear
 Kidney Antibodies  >1 publications with ROCK2 and Kidney
 Umbilical Cord Antibodies  >1 publications with ROCK2 and Umbilical Cord
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