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RNASE3 elisa kit :: Human Ribonuclease A3 (RNASE3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS455305 (SPECIAL PROMOTIONAL PRICING for a limited time)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $350 +1 FREE 8GB USB
96-Strip-Wells  /  $475 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,110 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,090 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ribonuclease A3 (RNASE3), ELISA Kit

★Popular Item★
 Also Known As   

Human Ribonuclease A3 (RNASE3) ELISA Kit

 Product Synonym Names    Rnase-A3; RnaseA3; ECP; RNS3; Ribonuclease,RNase A Family 3; Eosinophil Cationic Protein
 Product Gene Name   

RNASE3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    X15161 mRNA
 3D Structure    ModBase 3D Structure for P12724
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 Species Reactivity    Human
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 Specificity    This assay has high sensitivity and excellent specificity for detection of RNASE3. No significant cross-reactivity or interference between RNASE3 and analogues was observed.
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 Samples    Serum, plasma, cell lysates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    78.12-5000pg/mL
 Sensitivity    28.43pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNASE3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNASE3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of RNASE3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for RNASE3 purchase    MBS455305 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ribonuclease A3 (RNASE3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78.12-5000pg/mL in biological research samples containing RNASE3, with an estimated sensitivity of 28.43pg/mL. The ELISA analytical biochemical technique of the MBS455305 kit is based on RNASE3 antibody-RNASE3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RNASE3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RNASE3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for RNASE3 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNASE3 in human serum, plasma, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNASE3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RNASE3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNASE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RNASE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of RNASE3 elisa kit    RNASE3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS455305. Click to request current manual
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NCBI/Uniprot data below describe general gene information for RNASE3. It may not necessarily be applicable to this product.
 NCBI GI #    45243507
 NCBI GeneID    6037
 NCBI Accession #    NP_002926.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002935.2 [Other Products]
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 UniProt Primary Accession #    P12724 [Other Products]
 UniProt Secondary Accession #    Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9 [Other Products]
 UniProt Related Accession #    P12724 [Other Products]
 Molecular Weight    18,385 Da
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 NCBI Official Full Name    eosinophil cationic protein
 NCBI Official Synonym Full Names    ribonuclease A family member 3
 NCBI Official Symbol    RNASE3 [Similar Products]
 NCBI Official Synonym Symbols   
ECP; RAF1; RNS3
[Similar Products]
 NCBI Protein Information    eosinophil cationic protein
 UniProt Protein Name    Eosinophil cationic protein
 UniProt Synonym Protein Names   
Ribonuclease 3; RNase 3
 Protein Family    Eosinophil cationic protein
 UniProt Gene Name    RNASE3 [Similar Products]
 UniProt Synonym Gene Names    ECP; RNS3; ECP; RNase 3 [Similar Products]
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 NCBI Summary for RNASE3    The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
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 UniProt Comments for RNASE3    Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
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 Research Articles on RNASE3    1. Increased MRP8/14 levels suggest the instability of the atherosclerotic plaque. ECP reflects the severity of coronary arteries stenosis, predicting atherosclerosis burden. They may become the new biomarkers of CAD.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with RNASE3 elisa kitPathways associated with RNASE3 elisa kit
 Reference Product  PubMed Publications
 CCL11 elisa kit  >5 publications with RNASE3 and CCL11
 EPX elisa kit  >4 publications with RNASE3 and EPX
 IL5 elisa kit  >1 publications with RNASE3 and IL5
 Products by Pathway  Pathway Diagram
 Antimicrobial Peptides Pathway antibodies  Antimicrobial Peptides Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Neutrophil Degranulation Pathway antibodies  Neutrophil Degranulation Pathway Diagram
Diseases associated with RNASE3 elisa kitOrgans/Tissues associated with RNASE3 elisa kit
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >78 publications with RNASE3 and Hypersensitivity
 Respiratory Tract Diseases Antibodies  >72 publications with RNASE3 and Respiratory Tract Diseases
 Respiratory Hypersensitivity Antibodies  >61 publications with RNASE3 and Respiratory Hypersensitivity
 Asthma Antibodies  >60 publications with RNASE3 and Asthma
 Lung Diseases Antibodies  >53 publications with RNASE3 and Lung Diseases
 Inflammation Antibodies  >50 publications with RNASE3 and Inflammation
 Lung Diseases, Obstructive Antibodies  >50 publications with RNASE3 and Lung Diseases, Obstructive
 Rhinitis Antibodies  >25 publications with RNASE3 and Rhinitis
 Eosinophilia Antibodies  >21 publications with RNASE3 and Eosinophilia
 Rhinitis, Allergic, Seasonal Antibodies  >14 publications with RNASE3 and Rhinitis, Allergic, Seasonal
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >101 publications with RNASE3 and Blood
 Eye Antibodies  >3 publications with RNASE3 and Eye
 Brain Antibodies  >2 publications with RNASE3 and Brain
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